{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:21:48Z","timestamp":1785424908037,"version":"3.56.0"},"reference-count":56,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T00:00:00Z","timestamp":1758153600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T00:00:00Z","timestamp":1758153600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21AI175651"],"award-info":[{"award-number":["R21AI175651"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35GM139817"],"award-info":[{"award-number":["R35GM139817"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2025,9,18]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Chlamydia trachomatis<\/jats:italic>\n                      has a DNA topoisomerase I with a unique C-terminal domain (CTD) homologous to eukaryotic SWIB domains. This study focused on determining the function of the SWIB domain-containing TopA from\n                      <jats:italic toggle=\"yes\">C. trachomatis<\/jats:italic>\n                      (CtTopA). We demonstrated that, despite the lack of sequence similarity at the CTDs between CtTopA and TopA from\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      (EcTopA), full-length CtTopA removed negative DNA supercoils\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      and complemented the growth defect of a\n                      <jats:italic toggle=\"yes\">topA<\/jats:italic>\n                      mutant of\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      . CtTopA is less processive in DNA relaxation than EcTopA in dose-response and time course studies. An antibody generated against the SWIB domain of CtTopA specifically recognized CtTopA but not EcTopA or\n                      <jats:italic toggle=\"yes\">Mycobacterium tuberculosis<\/jats:italic>\n                      TopA, consistent with the sequence differences in their CTDs. The endogenous CtTopA protein is expressed at a relatively high level during the middle and late developmental stages of\n                      <jats:italic toggle=\"yes\">C. trachomatis<\/jats:italic>\n                      . Overexpressing a\n                      <jats:italic toggle=\"yes\">topA<\/jats:italic>\n                      mutant allele lacking the SWIB domain in\n                      <jats:italic toggle=\"yes\">C. trachomatis<\/jats:italic>\n                      resulted in slow growth when host protein synthesis was inhibited. These data suggest that productive infection of\n                      <jats:italic toggle=\"yes\">C. trachomatis<\/jats:italic>\n                      requires functional SWIB domain-containing CtTopA and\n                      <jats:italic toggle=\"yes\">de novo<\/jats:italic>\n                      host protein synthesis. Because SWIB domain-containing CtTopAs are not found in prokaryotes beyond\n                      <jats:italic toggle=\"yes\">Chlamydia<\/jats:italic>\n                      spp., our work suggests an important function of the SWIB domain on CtTopA activity during\n                      <jats:italic toggle=\"yes\">C. trachomatis<\/jats:italic>\n                      infection.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        <jats:italic toggle=\"yes\">Chlamydia trachomatis<\/jats:italic>\n                        is a medically important bacterial pathogen that is responsible for the most prevalent bacterial sexually transmitted infection. Bioinformatics, genetics, and biochemical analyses have established that the presence of a SWIB domain in CtTopA is relevant to chlamydial physiology. Our findings also underline the mechanistic diversity among the family of TopAs that are likely driven by pathogen-specific adaptations.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/jb.00190-25","type":"journal-article","created":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T13:00:57Z","timestamp":1755003657000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["The SWIB domain-containing DNA topoisomerase I of\n                    <i>Chlamydia trachomatis<\/i>\n                    mediates DNA relaxation"],"prefix":"10.1128","volume":"207","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9869-7317","authenticated-orcid":true,"given":"Li","family":"Shen","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01qv8fp92","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, Immunology, and 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