{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T17:49:47Z","timestamp":1792259387358,"version":"4.4.0"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2022,8,16]],"date-time":"2022-08-16T00:00:00Z","timestamp":1660608000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2022,8,16]],"date-time":"2022-08-16T00:00:00Z","timestamp":1660608000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP17K19769"],"award-info":[{"award-number":["JP17K19769"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP20K21684"],"award-info":[{"award-number":["JP20K21684"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2022,8,16]]},"abstract":"<jats:p>\n            The virulence factors of periodontal pathogens such as\n            <jats:named-content content-type=\"genus-species\">Prevotella intermedia<\/jats:named-content>\n            have not been elucidated. Using our established procedure, we succeeded in generating type IX secretion system mutants and gene complementation strains that might transfer virulence factors to the bacterial surface.\n          <\/jats:p>","DOI":"10.1128\/jb.00203-22","type":"journal-article","created":{"date-parts":[[2022,7,13]],"date-time":"2022-07-13T13:01:26Z","timestamp":1657717286000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Insertional Inactivation and Gene Complementation of Prevotella intermedia Type IX Secretion System Reveals Its Indispensable Roles in Black Pigmentation, Hemagglutination, Protease Activity of Interpain A, and Biofilm Formation"],"prefix":"10.1128","volume":"204","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0769-7747","authenticated-orcid":true,"given":"Mariko","family":"Naito","sequence":"first","affiliation":[{"name":"Department of Microbiology and Oral Infection, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7605-4721","authenticated-orcid":true,"given":"Mikio","family":"Shoji","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Oral Infection, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiko","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Frontier Oral Science, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koji","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Oral Infection, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0757.2002.280102.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0099-2399(07)80216-0"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0099-2399(99)80268-4"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1994.tb01172.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1982.53.4.223"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.1999.tb00081.x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1177\/10454411000110020201"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1902\/annals.2003.8.1.38"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1902\/annals.2001.6.1.164"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2010.137745"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.124.3.832"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00943-13"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.587235"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00510-09"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01361-13"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M708481200"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0014-5793(03)00414-9"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.49.100195.003431"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1177\/08959374970110010701"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.2006.00305.x"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2013.00303"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0912010107"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6968.12028"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr400487b"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/dnares\/dsv032"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00215"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.63.2.670-675.1997"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(03)00764-X"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2005.05.016"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/am.27.6.1031-1033.1974"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.1.196-202.1996"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302x.1989.tb00239.x"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2010.08.008"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9030551"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2000.tb08951.x"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302x.1989.tb00253.x"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20090343"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000316"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2012-13-6-r42"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12255"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0021372"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302x.1996.tb00335.x"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1111\/1348-0421.12838"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2415"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-9-11"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01691-06"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.080192-0"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0722.1982.tb01521.x"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.00203-22","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.00203-22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,16]],"date-time":"2022-08-16T13:02:17Z","timestamp":1660654937000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.00203-22"}},"subtitle":[],"editor":[{"given":"Conrad W.","family":"Mullineaux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,8,16]]},"references-count":48,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2022,8,16]]}},"alternative-id":["10.1128\/jb.00203-22"],"URL":"https:\/\/doi.org\/10.1128\/jb.00203-22","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,16]]},"assertion":[{"value":"2022-05-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00203-22"}}