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Pathogenic<jats:italic>Brucella<\/jats:italic>species adapted to an intracellular lifestyle have two genes involved in riboflavin synthesis,<jats:italic>ribH1<\/jats:italic>and<jats:italic>ribH2<\/jats:italic>, which are located on different chromosomes. The<jats:italic>ribH2<\/jats:italic>gene was shown previously to specify a lumazine synthase (type II lumazine synthase) with an unusual decameric structure and a very high<jats:italic>K<jats:sub>m<\/jats:sub><\/jats:italic>for 3,4-dihydroxy-2-butanone 4-phosphate. Moreover, the protein was found to be an immunodominant<jats:italic>Brucella<\/jats:italic>antigen and was able to generate strong humoral as well as cellular immunity against<jats:italic>Brucella abortus<\/jats:italic>in mice. We have now cloned and expressed the<jats:italic>ribH1<\/jats:italic>gene, which is located inside a small riboflavin operon, together with two other putative riboflavin biosynthesis genes and the<jats:italic>nusB<\/jats:italic>gene, specifying an antitermination factor. The RibH1 protein (type I lumazine synthase) is a homopentamer catalyzing the formation of 6,7-dimethyl-8-ribityllumazine at a rate of 18 nmol mg<jats:sup>\u22121<\/jats:sup>min<jats:sup>\u22121<\/jats:sup>. Sequence comparison of lumazine synthases from archaea, bacteria, plants, and fungi suggests a family of proteins comprising archaeal lumazine and riboflavin synthases, type I lumazine synthases, and the eubacterial type II lumazine synthases.<\/jats:p>","DOI":"10.1128\/jb.00207-06","type":"journal-article","created":{"date-parts":[[2006,8,21]],"date-time":"2006-08-21T17:14:16Z","timestamp":1156180456000},"page":"6135-6142","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["Evolution of Vitamin B<sub>2<\/sub>Biosynthesis: 6,7-Dimethyl-8-Ribityllumazine Synthases of<i>Brucella<\/i>"],"prefix":"10.1128","volume":"188","author":[{"given":"Vanesa","family":"Zylberman","sequence":"first","affiliation":[{"name":"Fundacio\u0301n Instituto Leloir, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastia\u0301n","family":"Klinke","sequence":"additional","affiliation":[{"name":"Fundacio\u0301n Instituto Leloir, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilka","family":"Haase","sequence":"additional","affiliation":[{"name":"Lehrstuhl fu\u0308r Organische Chemie und Biochemie Technische Universita\u0308t Mu\u0308nchen, Lichtenbergstra\u00dfe 4, D-85748 Garching, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adelbert","family":"Bacher","sequence":"additional","affiliation":[{"name":"Lehrstuhl fu\u0308r Organische Chemie und Biochemie Technische Universita\u0308t Mu\u0308nchen, Lichtenbergstra\u00dfe 4, D-85748 Garching, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"Fischer","sequence":"additional","affiliation":[{"name":"Lehrstuhl fu\u0308r Organische Chemie und Biochemie Technische Universita\u0308t Mu\u0308nchen, Lichtenbergstra\u00dfe 4, D-85748 Garching, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando Alberto","family":"Goldbaum","sequence":"additional","affiliation":[{"name":"Fundacio\u0301n Instituto Leloir, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/0076-6879(86)22170-9","volume":"122","year":"1986","unstructured":"Bacher, A. 1986. 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