{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T01:13:39Z","timestamp":1790039619123,"version":"4.0.1"},"reference-count":61,"publisher":"American Society for Microbiology","issue":"19","license":[{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100010713","name":"Indian Institute of Science Education and Research Bhopal","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010713","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:p>\n            <jats:named-content content-type=\"genus-species\">M. smegmatis<\/jats:named-content>\n            generates an inadequate amount of energy by substrate-level phosphorylation and mandatorily requires oxidative phosphorylation (OXPHOS) for its growth and metabolism. Here, we explored the influence of suboptimal OXPHOS on\n            <jats:named-content content-type=\"genus-species\">M. smegmatis<\/jats:named-content>\n            physiology and metabolism.\n            <jats:named-content content-type=\"genus-species\">M. smegmatis<\/jats:named-content>\n            harbors two identical copies of the\n            <jats:italic>atpD<\/jats:italic>\n            gene, which codes for the ATP synthase \u03b2 subunit. Here, we carried out the deletion of only one copy of\n            <jats:italic>atpD<\/jats:italic>\n            in\n            <jats:named-content content-type=\"genus-species\">M. smegmatis<\/jats:named-content>\n            to understand the bacterial survival response in an energy-deprived state.\n            <jats:named-content content-type=\"genus-species\">M. smegmatis<\/jats:named-content>\n            \u0394\n            <jats:italic>atpD<\/jats:italic>\n            shows remarkable phenotypic, metabolic, and physiological differences from the wild type. Our study thus establishes\n            <jats:named-content content-type=\"genus-species\">M. smegmatis<\/jats:named-content>\n            \u0394\n            <jats:italic>atpD<\/jats:italic>\n            as an energy-compromised mycobacterial strain, highlights the importance of ATP synthase in mycobacterial physiology, and further paves the way for the identification of novel antimycobacterial drug targets.\n          <\/jats:p>","DOI":"10.1128\/jb.00210-19","type":"journal-article","created":{"date-parts":[[2019,7,4]],"date-time":"2019-07-04T14:54:05Z","timestamp":1562252045000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["Insights into the Physiology and Metabolism of a Mycobacterial Cell in an Energy-Compromised State"],"prefix":"10.1128","volume":"201","author":[{"given":"Varsha","family":"Patil","sequence":"first","affiliation":[{"name":"Microbiology and Molecular Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER), Bhopal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0238-8144","authenticated-orcid":false,"given":"Vikas","family":"Jain","sequence":"additional","affiliation":[{"name":"Microbiology and Molecular Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER), Bhopal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","unstructured":"World Health Organization. 2018. 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