{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:05:27Z","timestamp":1790665527038,"version":"4.1.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"15","license":[{"start":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T00:00:00Z","timestamp":1501545600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:named-content content-type=\"genus-species\">Mycoplasma hominis<\/jats:named-content>lacks a cell wall, and lipoproteins anchored to the extracellular side of the plasma membrane are in direct contact with the host components. A Triton X-114 extract of<jats:named-content content-type=\"genus-species\">M. hominis<\/jats:named-content>enriched with lipoproteins was shown to stimulate the production of interleukin-23 (IL-23) by human dendritic cells (hDCs). The inflammasome activation of the host cell has never been reported upon<jats:named-content content-type=\"genus-species\">M. hominis<\/jats:named-content>infection. We studied here the interaction between<jats:named-content content-type=\"genus-species\">M. hominis<\/jats:named-content>PG21 and hDCs by analyzing both the inflammation-inducing mycoplasmal lipoproteins and the inflammasome activation of the host cell. IL-23-inducing lipoproteins were determined using a sequential extraction strategy with two nondenaturing detergents, Sarkosyl and Triton X-114, followed by SDS-PAGE separation and mass spectrometry identification. The activation of the hDC inflammasome was assessed using PCR array and enzyme-linked immunosorbent assay (ELISA). We defined a list of 24 lipoproteins that could induce the secretion of IL-23 by hDCs, 5 with a molecular mass between 20 and 35 kDa and 19 with a molecular mass between 40 and 100 kDa. Among them, lipoprotein MHO_4720 was identified as potentially bioactive, and a synthetic lipopeptide corresponding to the N-terminal part of the lipoprotein was subsequently shown to induce IL-23 release by hDCs. Regarding the hDC innate immune response, inflammasome activation with caspase-dependent production of IL-1\u03b2 was observed. After 24 h of coincubation of hDCs with<jats:named-content content-type=\"genus-species\">M. homini<\/jats:named-content>s, downregulation of the NLRP3-encoding gene and of the adaptor PYCARD-encoding gene was noticed. Overall, this study provides insight into both protagonists of the interaction of<jats:named-content content-type=\"genus-species\">M. hominis<\/jats:named-content>and hDCs.<\/jats:p><jats:p><jats:bold>IMPORTANCE<\/jats:bold><jats:named-content content-type=\"genus-species\">Mycoplasma hominis<\/jats:named-content>is a human urogenital pathogen involved in gynecologic and opportunistic infections.<jats:named-content content-type=\"genus-species\">M. hominis<\/jats:named-content>lacks a cell wall, and its membrane contains many lipoproteins that are anchored to the extracellular side of the plasma membrane. In the present study, we focused on the interaction between<jats:named-content content-type=\"genus-species\">M. hominis<\/jats:named-content>and human dendritic cells and examined both sides of the interaction, the mycoplasmal lipoproteins involved in the activation of the host cell and the immune response of the cell. On the mycoplasmal side, we showed for the first time that<jats:named-content content-type=\"genus-species\">M. hominis<\/jats:named-content>lipoproteins with high molecular mass were potentially bioactive. On the cell side, we reported an activation of the inflammasome, which is involved in the innate immune response.<\/jats:p>","DOI":"10.1128\/jb.00213-17","type":"journal-article","created":{"date-parts":[[2017,5,31]],"date-time":"2017-05-31T00:30:30Z","timestamp":1496190630000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Interaction of Mycoplasma hominis PG21 with Human Dendritic Cells: Interleukin-23-Inducing Mycoplasmal Lipoproteins and Inflammasome Activation of the Cell"],"prefix":"10.1128","volume":"199","author":[{"given":"J.","family":"Goret","sequence":"first","affiliation":[{"name":"Univ. Bordeaux, USC EA 3671 Mycoplasmal and chlamydial infections in humans, Bordeaux, France"},{"name":"INRA, USC EA 3671 Mycoplasmal and chlamydial infections in humans, Bordeaux, France"},{"name":"Laboratoire de Bact\u00e9riologie, CHU de Bordeaux, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"B\u00e9ven","sequence":"additional","affiliation":[{"name":"Univ. Bordeaux, INRA, UMR BFP, Villenave d'Ornon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Faustin","sequence":"additional","affiliation":[{"name":"Univ. Bordeaux, CNRS, UMR 5164, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Contin-Bordes","sequence":"additional","affiliation":[{"name":"Univ. Bordeaux, CNRS, UMR 5164, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Le Roy","sequence":"additional","affiliation":[{"name":"Univ. 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