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To evaluate the relative contributions of the sole catalase KatE and the peroxiredoxin AhpC produced by these strains in defense against H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>-mediated toxicity, isogenic<jats:italic>katE<\/jats:italic>,<jats:italic>ahpC<\/jats:italic>, and<jats:italic>katE ahpC<\/jats:italic>mutants were constructed and the phenotypic properties of these mutants compared with those of the virulent parental strain<jats:italic>B. abortus<\/jats:italic>2308. The results of these studies indicate that AhpC is the primary detoxifier of endogenous H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>generated by aerobic metabolism. KatE, on the other hand, plays a major role in scavenging exogenous and supraphysiologic levels of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, although this enzyme can play a supporting role in the detoxification of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>of endogenous origin if AhpC is absent.<jats:italic>B. abortus ahpC<\/jats:italic>and<jats:italic>katE<\/jats:italic>mutants exhibit wild-type virulence in C57BL\/6 and BALB\/c mice, but the<jats:italic>B. abortus ahpC katE<\/jats:italic>double mutant is extremely attenuated, and this attenuation is not relieved in derivatives of C57BL\/6 mice that lack NADPH oxidase (cybb) or inducible nitric oxide synthase (Nos2) activity. These experimental findings indicate that the generation of endogenous H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>represents a relevant environmental stress that<jats:italic>B. abortus<\/jats:italic>2308 must deal with during its residence in the host and that AhpC and KatE perform compensatory roles in detoxifying this metabolic H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>.<\/jats:p>","DOI":"10.1128\/jb.00231-10","type":"journal-article","created":{"date-parts":[[2010,7,31]],"date-time":"2010-07-31T01:45:04Z","timestamp":1280540704000},"page":"4912-4922","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":48,"title":["Comparative Study of the Roles of AhpC and KatE as Respiratory Antioxidants in<i>Brucella abortus<\/i>2308"],"prefix":"10.1128","volume":"192","author":[{"given":"Kendra H.","family":"Steele","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, North Carolina 27834"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John E.","family":"Baumgartner","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, North Carolina 27834"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle Wright","family":"Valderas","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, North Carolina 27834"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"II","given":"R. 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