{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T01:46:56Z","timestamp":1791942416548,"version":"4.3.4"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[2010,7,1]],"date-time":"2010-07-01T00:00:00Z","timestamp":1277942400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>VjbR is a LuxR-type quorum-sensing (QS) regulator that plays an essential role in the virulence of the intracellular facultative pathogen<jats:italic>Brucella<\/jats:italic>, the causative agent of brucellosis. It was previously described that VjbR regulates a diverse group of genes, including the<jats:italic>virB<\/jats:italic>operon. The latter codes for a type IV secretion system (T4SS) that is central for the pathogenesis of<jats:italic>Brucella<\/jats:italic>. Although the regulatory role of VjbR on the<jats:italic>virB<\/jats:italic>promoter (P<jats:sub><jats:italic>virB<\/jats:italic><\/jats:sub>) was extensively studied by different groups, the VjbR-binding site had not been identified so far. Here, we identified the target DNA sequence of VjbR in P<jats:sub><jats:italic>virB<\/jats:italic><\/jats:sub>by DNase I footprinting analyses. Surprisingly, we observed that VjbR specifically recognizes a sequence that is identical to a half-binding site of the QS-related regulator MrtR of<jats:italic>Mesorhizobium tianshanense<\/jats:italic>. As shown by DNase I footprinting and electrophoretic mobility shift assays, generation of a palindromic MrtR-like-binding site in P<jats:sub><jats:italic>virB<\/jats:italic><\/jats:sub>increased both the affinity and the stability of the VjbR-DNA complex, which confirmed that the QS regulator of<jats:italic>Brucella<\/jats:italic>is highly related to that of<jats:italic>M. tianshanense<\/jats:italic>. The addition of<jats:italic>N<\/jats:italic>-dodecanoyl homoserine lactone dissociated VjbR from the promoter, which confirmed previous reports that indicated a negative effect of this signal on the VjbR-mediated activation of P<jats:sub><jats:italic>virB<\/jats:italic><\/jats:sub>. Our results provide new molecular evidence for the structure of the<jats:italic>virB<\/jats:italic>promoter and reveal unusual features of the QS target DNA sequence of the main regulator of virulence in<jats:italic>Brucella<\/jats:italic>.<\/jats:p>","DOI":"10.1128\/jb.00232-10","type":"journal-article","created":{"date-parts":[[2010,4,17]],"date-time":"2010-04-17T01:46:10Z","timestamp":1271468770000},"page":"3434-3440","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":39,"title":["Identification of the Quorum-Sensing Target DNA Sequence and<i>N<\/i>-Acyl Homoserine Lactone Responsiveness of the<i>Brucella abortus virB<\/i>promoter"],"prefix":"10.1128","volume":"192","author":[{"given":"Gasto\u0301n M.","family":"Arocena","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Biotecnolo\u0301gicas, Universidad Nacional de General San Marti\u0301n, IIB-INTECH-CONICET, San Marti\u0301n 1650, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo","family":"Sieira","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnolo\u0301gicas, Universidad Nacional de General San Marti\u0301n, IIB-INTECH-CONICET, San Marti\u0301n 1650, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego J.","family":"Comerci","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnolo\u0301gicas, Universidad Nacional de General San Marti\u0301n, IIB-INTECH-CONICET, San Marti\u0301n 1650, Buenos Aires, Argentina"},{"name":"Comisio\u0301n Nacional de Energi\u0301a Ato\u0301mica, Divisio\u0301n Agropecuaria, Centro Ato\u0301mico Ezeiza 1804, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolfo A.","family":"Ugalde","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnolo\u0301gicas, Universidad Nacional de General San Marti\u0301n, IIB-INTECH-CONICET, San Marti\u0301n 1650, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1006\/jmbi.2001.5294","volume":"315","year":"2002","unstructured":"Bae, T., B. 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