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Quorum sensing (QS) is a global regulation process based on the synthesis of, detection of, and response to small diffusible molecules, called<jats:italic>N<\/jats:italic>-acyl-homoserine lactones (AHL), in gram-negative bacteria. In numerous species, QS has been shown to regulate genes involved in the establishment of pathogenic interactions with the host.<jats:italic>Brucella melitensis<\/jats:italic>produces<jats:italic>N<\/jats:italic>-dodecanoyl homoserine lactones (C<jats:sub>12<\/jats:sub>-HSL), which down regulate the expression of flagellar genes and of the<jats:italic>virB<\/jats:italic>operon (encoding a type IV secretion system), both of which encode surface virulence factors. A QS-related regulator, called VjbR, was identified as a transcriptional activator of these genes. We hypothesized that VjbR mediates the C<jats:sub>12<\/jats:sub>-HSL effects described above.<jats:italic>vjbR<\/jats:italic>alleles mutated in the region coding for the AHL binding domain were constructed to test this hypothesis. These alleles expressed in<jats:italic>trans<\/jats:italic>in a \u0394<jats:italic>vjbR<\/jats:italic>background behave as constitutive regulators both in vitro and in a cellular model of infection. Interestingly, the resulting<jats:italic>B. melitensis<\/jats:italic>strains, unable to respond to AHLs, aggregate spontaneously in liquid culture. Preliminary characterization of these strains showed altered expression of some outer membrane proteins and overproduction of a matrix-forming exopolysaccharide, suggesting for the first time that<jats:italic>B. melitensis<\/jats:italic>could form biofilms. Together, these results indicate that QS through VjbR is a major regulatory system of important cell surface structures of<jats:italic>Brucella<\/jats:italic>and as such plays a key role in host-pathogen interactions.<\/jats:p>","DOI":"10.1128\/jb.00265-07","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T04:56:59Z","timestamp":1181365019000},"page":"6035-6047","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":75,"title":["Mutations of the Quorum Sensing-Dependent Regulator VjbR Lead to Drastic Surface Modifications in<i>Brucella melitensis<\/i>"],"prefix":"10.1128","volume":"189","author":[{"given":"Sophie","family":"Uzureau","sequence":"first","affiliation":[{"name":"Unite\u0301 de Recherche en Biologie Mole\u0301culaire, Laboratoire d'Immunologie-Microbiologie, Faculte\u0301s Universitaires Notre-Dame de la Paix, Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Godefroid","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Recherche en Biologie Mole\u0301culaire, Laboratoire d'Immunologie-Microbiologie, Faculte\u0301s Universitaires Notre-Dame de la Paix, Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chantal","family":"Deschamps","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Recherche en Biologie Mole\u0301culaire, Laboratoire d'Immunologie-Microbiologie, Faculte\u0301s Universitaires Notre-Dame de la Paix, Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Lemaire","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Recherche en Biologie Mole\u0301culaire, Laboratoire d'Immunologie-Microbiologie, Faculte\u0301s Universitaires Notre-Dame de la Paix, Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"De Bolle","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Recherche en Biologie Mole\u0301culaire, Laboratoire d'Immunologie-Microbiologie, Faculte\u0301s Universitaires Notre-Dame de la Paix, Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Jacques","family":"Letesson","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Recherche en Biologie Mole\u0301culaire, Laboratoire d'Immunologie-Microbiologie, Faculte\u0301s Universitaires Notre-Dame de la Paix, Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Current protocols in molecular biology. 1991"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.10.5693-5703.2004"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1111\/j.1365-2958.1995.tb02361.x","volume":"15","year":"1995","unstructured":"Bugert, P., and K. 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