{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T17:18:55Z","timestamp":1788369535448,"version":"build-2803163510"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T00:00:00Z","timestamp":1738281600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T00:00:00Z","timestamp":1738281600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2025,1,31]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Chlamydia pneumoniae<\/jats:italic>\n              is an obligate intracellular bacterium of eukaryotic cells characterized by a unique biphasic life cycle; its biosynthesis and replication must occur within a cytoplasmic vacuole or inclusion. Certain inclusion membrane proteins have been demonstrated to mediate the interactions between intra-inclusion chlamydial organisms and the host cell. It has been demonstrated previously that the\n              <jats:italic>C. pneumoniae<\/jats:italic>\n              -encoded Cpn0308 localizes to the inclusion membrane; however, its function remains unknown. In the current study, a yeast two-hybrid assay was conducted to screen Cpn0308 as a bait against a HeLa cell cDNA library, revealing its binding to the host protein acyl-coenzyme A binding domain-containing 3 (ACBD3). The interaction between Cpn0308 and ACBD3 was confirmed through co-immunoprecipitation and GST (Glutathione S-transferase) pull-down assays. The two proteins were also co-localized in HeLa cells co-expressing Cpn0308 and ACBD3, as well as in\n              <jats:italic>C. pneumoniae<\/jats:italic>\n              -infected cells, as observed under confocal fluorescence microscopy. Given that ACBD3 plays a crucial role in maintaining host cell lipid homeostasis and its Golgi dynamic domain is responsible for interacting with Cpn0308, we hypothesize that the Cpn0308-ACBD3 interaction may facilitate\n              <jats:italic>C. pneumoniae<\/jats:italic>\n              \u2019s acquisition of host lipids, thereby benefiting chlamydial survival. This study lays a foundation for further elucidating the mechanisms of Cpn0308-mediated\n              <jats:italic>C. pneumoniae<\/jats:italic>\n              pathogenesis.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                The biosynthesis and replication of\n                <jats:italic>Chlamydia pneumoniae<\/jats:italic>\n                (\n                <jats:italic>Cpn<\/jats:italic>\n                ) must occur within the cytoplasmic vacuoles or inclusions of host cells. Inclusion bodies play a crucial role in mediating the interactions between\n                <jats:italic>Cpn<\/jats:italic>\n                and host cells. Cpn0308 is localized to the inclusion membrane; however, its function is unknown. In this study, Cpn0308 was found to bind to host protein acyl-coenzyme A binding domain-containing 3 (ACBD3) through some standard approaches. Co-localization of the two proteins was observed in both original HeLa cells and Cpn-infected HeLa cells. ACBD3 plays a significant role in maintaining lipid homeostasis in host cells; we speculate that the Cpn0308-ACBD3 interaction may facilitate the acquisition of host lipids by\n                <jats:italic>C. pneumoniae<\/jats:italic>\n                , thereby enhancing chlamydial survival.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/jb.00275-24","type":"journal-article","created":{"date-parts":[[2024,12,26]],"date-time":"2024-12-26T14:00:18Z","timestamp":1735221618000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["The\n            <i>Chlamydia pneumoniae<\/i>\n            inclusion membrane protein Cpn0308 interacts with host protein ACBD3"],"prefix":"10.1128","volume":"207","author":[{"given":"Liang","family":"Ma","sequence":"first","affiliation":[{"name":"Pathogen Biology and Immunology Research Institute, Hebei North University","place":["Zhangjiakou, China"]},{"name":"Key Laboratory of Clinical Laboratory Diagnostics, Hebei North University","place":["Zhangjiakou, China"]},{"name":"Handan Vocational College of Science and Technology","place":["Han Dan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-hui","family":"Jia","sequence":"additional","affiliation":[{"name":"Pathogen Biology and Immunology Research Institute, Hebei North University","place":["Zhangjiakou, China"]},{"name":"Key Laboratory of Clinical Laboratory Diagnostics, Hebei North University","place":["Zhangjiakou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Gao","sequence":"additional","affiliation":[{"name":"Pathogen Biology and Immunology Research Institute, Hebei North University","place":["Zhangjiakou, China"]},{"name":"Key Laboratory of Clinical Laboratory Diagnostics, Hebei North University","place":["Zhangjiakou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Pathogen Biology and Immunology Research Institute, Hebei North University","place":["Zhangjiakou, China"]},{"name":"Key Laboratory of Clinical Laboratory Diagnostics, Hebei North University","place":["Zhangjiakou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-ting","family":"Cheng","sequence":"additional","affiliation":[{"name":"Pathogen Biology and Immunology Research Institute, Hebei North University","place":["Zhangjiakou, China"]},{"name":"Key Laboratory of Clinical Laboratory Diagnostics, Hebei North University","place":["Zhangjiakou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Laboratory Diagnostics, Hebei North University","place":["Zhangjiakou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8903-1107","authenticated-orcid":true,"given":"Tian-jun","family":"Jia","sequence":"additional","affiliation":[{"name":"Pathogen Biology and Immunology Research Institute, Hebei North University","place":["Zhangjiakou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1159\/000506460"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-019-01273-x"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0250034"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12931-021-01635-w"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/82_2016_18"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-06749-9"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1096330"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsre.2005.03.002"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/b93995"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.565808"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1134\/S1990750808020042"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1286-4579(02)01546-0"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1010818"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0185593"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00935-06"},{"key":"e_1_3_4_17_2","first-page":"219","article-title":"Study on gene cloning of Chlamydial pneumonia CPn0308 and its endogenous localization","volume":"37","author":"Jia T","year":"2008","unstructured":"Jia T, Liu D, Luo J, Zhang S, et al.. 2008. 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