{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T01:11:29Z","timestamp":1768698689608,"version":"3.49.0"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2018,9,1]],"date-time":"2018-09-01T00:00:00Z","timestamp":1535760000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001412","name":"Council of Scientific and Industrial Research","doi-asserted-by":"publisher","award":["27(0325)\/17\/EMR-II"],"award-info":[{"award-number":["27(0325)\/17\/EMR-II"]}],"id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology , Ministry of Science and Technology","doi-asserted-by":"publisher","award":["BT\/PR20257\/BBE\/117\/223\/2016"],"award-info":[{"award-number":["BT\/PR20257\/BBE\/117\/223\/2016"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:p>\n            Methylotrophy, the ability to utilize single-carbon (C\n            <jats:sub>1<\/jats:sub>\n            ) compounds as the sole carbon and energy sources, is only poorly understood in mycobacteria. Both pathogenic and nonpathogenic mycobacteria, including\n            <jats:named-content content-type=\"genus-species\">Mycobacterium tuberculosis<\/jats:named-content>\n            , are capable of utilizing C\n            <jats:sub>1<\/jats:sub>\n            compounds to meet their carbon and energy requirements, although the precise pathways are not well studied. Here we present a comprehensive study of methylotrophy in\n            <jats:named-content content-type=\"genus-species\">Mycobacterium smegmatis<\/jats:named-content>\n            . With several genetic knockouts, we have dissected the entire methanol metabolism pathway in\n            <jats:named-content content-type=\"genus-species\">M. smegmatis<\/jats:named-content>\n            . We show that while methanol dissimilation in\n            <jats:named-content content-type=\"genus-species\">M. smegmatis<\/jats:named-content>\n            differs from that in other mycobacterial species, the concluding step of CO\n            <jats:sub>2<\/jats:sub>\n            fixation is similar to that in\n            <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n            . It is therefore both interesting and important to examine mycobacterial physiology in the presence of alternative carbon sources.\n          <\/jats:p>","DOI":"10.1128\/jb.00288-18","type":"journal-article","created":{"date-parts":[[2018,6,12]],"date-time":"2018-06-12T09:55:23Z","timestamp":1528797323000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["Methylotrophy in Mycobacteria: Dissection of the Methanol Metabolism Pathway in Mycobacterium smegmatis"],"prefix":"10.1128","volume":"200","author":[{"given":"Abhishek Anil","family":"Dubey","sequence":"first","affiliation":[{"name":"Microbiology and Molecular Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER), Bhopal, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saloni Rajesh","family":"Wani","sequence":"additional","affiliation":[{"name":"Microbiology and Molecular Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER), Bhopal, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0238-8144","authenticated-orcid":false,"given":"Vikas","family":"Jain","sequence":"additional","affiliation":[{"name":"Microbiology and Molecular Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER), Bhopal, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"79","article-title":"Assimilation of carbon by methylotrophs","volume":"18","author":"Anthony C","year":"1991","unstructured":"Anthony C. 1991. 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