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Treatment failure is attributed in part to antibiotic-tolerant phenotypic variants known as persister cells. Understanding how these cells emerge will likely inform future therapeutic strategies. In the current study, we identified\n            <jats:italic>carB<\/jats:italic>\n            , which codes for the large subunit of carbamoyl-phosphate synthetase, as a persister gene that contributes to multidrug tolerance in\n            <jats:named-content content-type=\"genus-species\">P. aeruginosa<\/jats:named-content>\n            . Disruption of\n            <jats:italic>carB<\/jats:italic>\n            resulted in a metabolic perturbation that increased cellular ATP and reduced persister formation. Conversely, lowering ATP in the mutant restored antibiotic tolerance. Our data support the hypothesis that a drop in intracellular ATP is a general mechanism of persister formation in bacteria.\n          <\/jats:p>","DOI":"10.1128\/jb.00303-18","type":"journal-article","created":{"date-parts":[[2018,6,25]],"date-time":"2018-06-25T13:32:02Z","timestamp":1529933522000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":76,"title":["A Genetic Determinant of Persister Cell Formation in Bacterial Pathogens"],"prefix":"10.1128","volume":"200","author":[{"given":"David R.","family":"Cameron","sequence":"first","affiliation":[{"name":"Antimicrobial Discovery Center, Department of Biology, Northeastern University, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Shan","sequence":"additional","affiliation":[{"name":"Antimicrobial Discovery Center, Department of Biology, Northeastern University, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliza A.","family":"Zalis","sequence":"additional","affiliation":[{"name":"Antimicrobial Discovery Center, Department of Biology, Northeastern University, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Isabella","sequence":"additional","affiliation":[{"name":"Antimicrobial Discovery Center, Department of Biology, Northeastern University, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1335-9982","authenticated-orcid":false,"given":"Kim","family":"Lewis","sequence":"additional","affiliation":[{"name":"Antimicrobial Discovery Center, Department of Biology, Northeastern University, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.112408.134306"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature12790"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature14662"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01651-09"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200304-505SO"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.155.2.768-775.1983"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01740-05"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2013.08.045"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000317"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.51"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02267-16"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01964-17"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep20519"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00078-15"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1227"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00369-06"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00144-08"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.061192-0"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.9.2242-2246.2000"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2004.tb09732.x"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.9.2021"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00047-06"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01917"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)74210-3"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-6-53"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1557"},{"key":"e_1_3_3_28_2","first-page":"1297","article-title":"The rate of killing of Escherichia coli by beta-lactam antibiotics is strictly proportional to the rate of bacterial growth","volume":"132","author":"Tuomanen E","year":"1986","unstructured":"Tuomanen E, Cozens R, Tosch W, Zak O, Tomasz A. 1986. 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