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Both organisms are adapted to grow under oxygen-limited conditions in rice fields by generating energy through denitrification. Microoxic growth of\n            <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n            is also considered essential for human infections. Here, we have used a Tn-Seq approach to identify the genes encoding the enzymes and regulators required for growth under denitrifying conditions. We show that a mutant that is defective in the conversion of N\n            <jats:sub>2<\/jats:sub>\n            O to N\n            <jats:sub>2<\/jats:sub>\n            , the last step in the denitrification process, is unaffected in microoxic growth but is severely impaired in biofilm formation, suggesting that N\n            <jats:sub>2<\/jats:sub>\n            O may play a role in biofilm dispersal. Our study identified novel targets for the development of therapeutic agents to treat meliodiosis.\n          <\/jats:p>","DOI":"10.1128\/jb.00304-20","type":"journal-article","created":{"date-parts":[[2020,9,7]],"date-time":"2020-09-07T13:20:05Z","timestamp":1599484805000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Mapping of the Denitrification Pathway in Burkholderia thailandensis by Genome-Wide Mutant Profiling"],"prefix":"10.1128","volume":"202","author":[{"given":"Alessandra","family":"Vitale","sequence":"first","affiliation":[{"name":"Department of Plant and Microbial Biology, University of Zurich, Z\u00fcrich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Paszti","sequence":"additional","affiliation":[{"name":"Department of Plant and Microbial Biology, University of Zurich, Z\u00fcrich, 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