{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T01:18:44Z","timestamp":1782523124762,"version":"3.54.5"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"20","license":[{"start":{"date-parts":[[2020,9,23]],"date-time":"2020-09-23T00:00:00Z","timestamp":1600819200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["MCB-1120346"],"award-info":[{"award-number":["MCB-1120346"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["MCB-1153413"],"award-info":[{"award-number":["MCB-1153413"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"USDA | National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["VA-160115"],"award-info":[{"award-number":["VA-160115"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2020,9,23]]},"abstract":"<jats:p>\n            <jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>\n            is the most reported sexually transmitted infection in the United States and the leading cause of infectious blindness worldwide. Unlike many other intracellular pathogens that have undergone reductive evolution,\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            is capable of\n            <jats:italic>de novo<\/jats:italic>\n            biosynthesis of the essential cofactor tetrahydrofolate using a noncanonical pathway. Here, we identify the biosynthetic precursor to the\n            <jats:italic>p<\/jats:italic>\n            -aminobenzoate (pABA) portion of folate in a process that requires the CT610 enzyme from\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            . We further provide evidence that CT610 is a self-sacrificing or \u201csuicide\u201d enzyme that uses its own amino acid residue(s) as the substrate for pABA synthesis. This work provides the foundation for future investigation of this chlamydial pABA synthase, which could lead to new therapeutic strategies for\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            infections.\n          <\/jats:p>","DOI":"10.1128\/jb.00319-20","type":"journal-article","created":{"date-parts":[[2020,7,27]],"date-time":"2020-07-27T12:39:16Z","timestamp":1595853556000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["An Unusual Route for\n            <i>p<\/i>\n            -Aminobenzoate Biosynthesis in Chlamydia trachomatis Involves a Probable Self-Sacrificing Diiron Oxygenase"],"prefix":"10.1128","volume":"202","author":[{"given":"Yamilet","family":"Macias-Orihuela","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Cast","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Gonzaga University, Spokane, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian","family":"Crawford","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Gonzaga University, Spokane, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin J.","family":"Brandecker","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Gonzaga University, Spokane, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer J.","family":"Thiaville","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences and Genetics Institute, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexey G.","family":"Murzin","sequence":"additional","affiliation":[{"name":"MRC Laboratory of Molecular Biology, Cambridge, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"de Cr\u00e9cy-Lagard","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences and Genetics Institute, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert H.","family":"White","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6860-659X","authenticated-orcid":false,"given":"Kylie D.","family":"Allen","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","volume-title":"One-carbon metabolism","author":"Matthews RG","year":"1996","unstructured":"Matthews RG. 1996. 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