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Using DNA affinity chromatography, two proteins were found to bind to the promoter region of\n                    <jats:italic>ykrL<\/jats:italic>\n                    : Rok, known as a repressor of competence and genes for extracytoplasmic functions, and YkrK, a novel type of regulator encoded by the gene adjacent to\n                    <jats:italic>ykrL<\/jats:italic>\n                    but divergently transcribed. Electrophoretic mobility shift assays showed Rok and YkrK binding to the\n                    <jats:italic>ykrL<\/jats:italic>\n                    promoter region as well as YkrK binding to the\n                    <jats:italic>ykrK<\/jats:italic>\n                    promoter region. Comparative bioinformatic analysis of the\n                    <jats:italic>ykrL<\/jats:italic>\n                    promoter regions in related\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Bacillus<\/jats:named-content>\n                    species revealed a consensus motif, which was demonstrated to be the binding site of YkrK. Deletion of\n                    <jats:italic>rok<\/jats:italic>\n                    and\n                    <jats:italic>ykrK<\/jats:italic>\n                    in a P\n                    <jats:italic>ykrL<\/jats:italic>\n                    -\n                    <jats:italic>gfp<\/jats:italic>\n                    reporter strain showed that both proteins are repressors of\n                    <jats:italic>ykrL<\/jats:italic>\n                    expression. In addition, conditions which activated P\n                    <jats:italic>ykrL<\/jats:italic>\n                    (membrane protein overproduction, dissipation of the membrane potential, and salt and phenol stress) point to the involvement of YkrL in membrane protein quality control.\n                  <\/jats:p>","DOI":"10.1128\/jb.00324-12","type":"journal-article","created":{"date-parts":[[2012,3,24]],"date-time":"2012-03-24T03:30:52Z","timestamp":1332559852000},"page":"2837-2845","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Regulation of\n                    <i>ykrL<\/i>\n                    (\n                    <i>htpX<\/i>\n                    ) by Rok and YkrK, a Novel Type of Regulator in Bacillus subtilis"],"prefix":"10.1128","volume":"194","author":[{"given":"Bogumi\u0142a C.","family":"Marciniak","sequence":"first","affiliation":[{"name":"Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hein","family":"Trip","sequence":"additional","affiliation":[{"name":"Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrizia","family":"Fusetti","sequence":"additional","affiliation":[{"name":"Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands"},{"name":"Netherlands Proteomics Centre, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar P.","family":"Kuipers","sequence":"additional","affiliation":[{"name":"Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands"},{"name":"Kluyver Center for Genomics of Industrial Fermentation, Delft\/Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1093\/jb\/mvp071","article-title":"Quality control of cytoplasmic membrane proteins in Escherichia coli","volume":"146","author":"Akiyama Y","year":"2009","unstructured":"AkiyamaY. 2009. 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