{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:32:05Z","timestamp":1787016725225,"version":"build-2736575974"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[2006,7,1]],"date-time":"2006-07-01T00:00:00Z","timestamp":1151712000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    We describe identification and functional characterization of ISEc\n                    <jats:italic>11<\/jats:italic>\n                    , a new insertion sequence that is widespread in enteroinvasive\n                    <jats:italic>E. coli<\/jats:italic>\n                    (EIEC), in which it is always present on the virulence plasmid (pINV) and very frequently also present on the chromosome. ISEc\n                    <jats:italic>11<\/jats:italic>\n                    is flanked by subterminal 13-bp inverted repeats (IRs) and is bounded by 3-bp terminal sequences, and it transposes with target specificity without generating duplication of the target site. ISEc\n                    <jats:italic>11<\/jats:italic>\n                    is characterized by an atypical transposase containing the DEDD motif of the Piv\/MooV family of DNA recombinases, and it is closely related to the IS\n                    <jats:italic>1111<\/jats:italic>\n                    family. Transposition occurs by formation of minicircles through joining of the abutted ends and results in assembly of a junction promoter (P\n                    <jats:sub>juncC<\/jats:sub>\n                    ) containing a \u221210 box in the interstitial sequence and a \u221235 box upstream of the right IR. A natural variant of ISEc\n                    <jats:italic>11<\/jats:italic>\n                    (ISEc\n                    <jats:italic>11<\/jats:italic>\n                    p), found on EIEC pINV plasmids, contains a perfect duplication of the outermost 39 bp of the right end. Upon circularization, ISEc\n                    <jats:italic>11<\/jats:italic>\n                    p forms a junction promoter (P\n                    <jats:sub>juncP<\/jats:sub>\n                    ) which, despite carrying \u221210 and \u221235 boxes identical to those of P\n                    <jats:sub>juncC<\/jats:sub>\n                    , exhibits 30-fold-greater strength in vivo. The discovery of only one starting point in primer extension experiments rules out the possibility that there are alternative promoter sites within the 39-bp duplication. Analysis of in vitro-generated transcripts confirmed that at limiting RNA polymerase concentrations, the activity of P\n                    <jats:sub>juncP<\/jats:sub>\n                    is 20-fold higher than the activity of P\n                    <jats:sub>juncC<\/jats:sub>\n                    . These observations suggest that the 39-bp duplication might host\n                    <jats:italic>cis<\/jats:italic>\n                    -acting elements that facilitate the binding of RNA polymerase to the promoter.\n                  <\/jats:p>","DOI":"10.1128\/jb.00332-06","type":"journal-article","created":{"date-parts":[[2006,6,20]],"date-time":"2006-06-20T19:15:17Z","timestamp":1150830917000},"page":"4681-4689","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Plasticity of the P\n                    <sub>junc<\/sub>\n                    Promoter of ISEc\n                    <i>11<\/i>\n                    , a New Insertion Sequence of the IS\n                    <i>1111<\/i>\n                    Family"],"prefix":"10.1128","volume":"188","author":[{"given":"Gianni","family":"Prosseda","sequence":"first","affiliation":[{"name":"Dipartimento Biologia Cellulare e dello Sviluppo, Universita\u0300 La Sapienza, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Carmela","family":"Latella","sequence":"additional","affiliation":[{"name":"Dipartimento Biologia Cellulare e dello Sviluppo, Universita\u0300 La Sapienza, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariassunta","family":"Casalino","sequence":"additional","affiliation":[{"name":"Dipartimento Biologia, Universita\u0300 Roma 3, 00146 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauro","family":"Nicoletti","sequence":"additional","affiliation":[{"name":"Dipartimento Scienze Biomediche, Universita\u0300 G. D'Annunzio, 66100 Chieti, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Michienzi","sequence":"additional","affiliation":[{"name":"Dipartimento Biologia, Universita\u0300 Roma 3, 00146 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bianca","family":"Colonna","sequence":"additional","affiliation":[{"name":"Dipartimento Biologia Cellulare e dello Sviluppo, Universita\u0300 La Sapienza, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.5.1829-1834.2006"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1099\/13500872-145-5-1201","volume":"145","year":"1999","unstructured":"Bhatt, A., and T. Keiser. 1999. Transposition of IS117 of Streptomyces coelicoor A3(2) in Mycobacterium smegmatis. 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