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Despite recent studies demonstrating\n                      <jats:italic toggle=\"yes\">A. caviae<\/jats:italic>\n                      is the most predominant\n                      <jats:italic toggle=\"yes\">Aeromonas<\/jats:italic>\n                      species underlying human infection,\n                      <jats:italic toggle=\"yes\">A. caviae<\/jats:italic>\n                      remains understudied, and no\n                      <jats:italic toggle=\"yes\">A. caviae<\/jats:italic>\n                      -specific virulence factors associated with human disease have been identified. To identify\n                      <jats:italic toggle=\"yes\">A. caviae<\/jats:italic>\n                      -specific putative virulence factors, we conducted comparative genomic analyses among clinical\n                      <jats:italic toggle=\"yes\">Aeromonas<\/jats:italic>\n                      isolates (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 431), which identified a variant of\n                      <jats:italic toggle=\"yes\">flgB,<\/jats:italic>\n                      predicted to encode a polar flagellum machinery protein, as over-represented in\n                      <jats:italic toggle=\"yes\">A. caviae<\/jats:italic>\n                      isolates. To examine the role of\n                      <jats:italic toggle=\"yes\">flgB<\/jats:italic>\n                      in virulence and host\u2013pathogen interactions, we generated an\n                      <jats:italic toggle=\"yes\">A. caviae flgB<\/jats:italic>\n                      deletion mutant and genetic complementation constructs. Swimming motility and polar flagella assembly were abolished in the mutant and functionally rescued with genetic complementation. As it remains unknown where\n                      <jats:italic toggle=\"yes\">A. caviae<\/jats:italic>\n                      infects the human gastrointestinal tract, we assessed host\u2013pathogen interactions in HT-29 and Caco2 human intestinal cell lines, representative of the large and small intestine, respectively. Deletion of\n                      <jats:italic toggle=\"yes\">flgB<\/jats:italic>\n                      significantly decreased bacterial adherence in only HT-29 cells and also decreased production of proinflammatory cytokines, IL-8, IL-13, IL-1\u03b2, and IL-6, by both cell types. Given the lack of relevant mammalian models for studying most enteric pathogens\n                      <jats:italic toggle=\"yes\">in vivo,<\/jats:italic>\n                      we characterized\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      virulence in a\n                      <jats:italic toggle=\"yes\">Galleria mellonella<\/jats:italic>\n                      larval survival model, where the\n                      <jats:italic toggle=\"yes\">flgB<\/jats:italic>\n                      deletion modestly attenuated virulence. Deletion of\n                      <jats:italic toggle=\"yes\">flgB<\/jats:italic>\n                      altered aspects of virulence and host\u2013pathogen interactions, and this study provides a framework for identification and characterization of\n                      <jats:italic toggle=\"yes\">A. caviae<\/jats:italic>\n                      -specific putative virulence factors.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        <jats:italic toggle=\"yes\">Aeromonas caviae<\/jats:italic>\n                        is an emerging human bacterial pathogen associated with gastroenteritis, wound infections, and numerous other infectious diseases. Recent studies demonstrate that\n                        <jats:italic toggle=\"yes\">A. caviae<\/jats:italic>\n                        accounts for the greatest burden of human\n                        <jats:italic toggle=\"yes\">Aeromonas<\/jats:italic>\n                        infections. Despite this,\n                        <jats:italic toggle=\"yes\">A. caviae<\/jats:italic>\n                        is understudied as a human pathogen. To address this gap in knowledge, this study characterizes\n                        <jats:italic toggle=\"yes\">A. caviae-<\/jats:italic>\n                        specific virulence genes. We examined 431 clinical\n                        <jats:italic toggle=\"yes\">Aeromonas<\/jats:italic>\n                        isolates using comparative genomics and identified and functionally characterized a putative\n                        <jats:italic toggle=\"yes\">A<\/jats:italic>\n                        . caviae-specific virulence factor\n                        <jats:italic toggle=\"yes\">, flgB<\/jats:italic>\n                        . Genetic deletion of\n                        <jats:italic toggle=\"yes\">flgB<\/jats:italic>\n                        in\n                        <jats:italic toggle=\"yes\">A. caviae<\/jats:italic>\n                        resulted in deficiencies in bacterial motility, adherence, host-cell proinflammatory cytokine production, and\n                        <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                        virulence in an invertebrate model. This work establishes the foundation for further study of additional\n                        <jats:italic toggle=\"yes\">A. caviae-<\/jats:italic>\n                        specific virulence factors.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/jb.00339-25","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T13:00:26Z","timestamp":1767013226000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Characterization of the role of putative\n                    <i>Aeromonas caviae<\/i>\n                    -specific virulence factor,\n                    <i>flgB,<\/i>\n                    in virulence and host\u2013pathogen interactions"],"prefix":"10.1128","volume":"208","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-9407-2957","authenticated-orcid":false,"given":"Bernadette 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