{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T14:21:08Z","timestamp":1788445268923,"version":"build-2803163510"},"reference-count":28,"publisher":"American Society for Microbiology","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100007155","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MC_PC_12025"],"award-info":[{"award-number":["MC_PC_12025"]}],"id":[{"id":"10.13039\/501100007155","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007155","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/W018756\/1"],"award-info":[{"award-number":["MR\/W018756\/1"]}],"id":[{"id":"10.13039\/501100007155","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Mycobacterium tuberculosis<\/jats:italic>\n                      (Mtb) causes over 1 million deaths from tuberculosis (TB) every year and remains a major burden on human health. Reducing the deadly impact of TB requires a better understanding of the strategies used by\n                      <jats:italic toggle=\"yes\">M. tuberculosis<\/jats:italic>\n                      to adapt its metabolism, survive, and persist in the human host. Previous enzymological studies reported that the Mtb\n                      <jats:italic toggle=\"yes\">rv3400<\/jats:italic>\n                      gene encodes a \u03b2-phosphoglucomutase; however, its role in\n                      <jats:italic toggle=\"yes\">M. tuberculosis<\/jats:italic>\n                      metabolism had not been investigated. Here, we show that deletion of\n                      <jats:italic toggle=\"yes\">rv3400<\/jats:italic>\n                      causes a 30-fold increase in \u03b2-D-glucose-1-phosphate, confirming its primary function as a \u03b2-phosphoglucomutase. Deletion of\n                      <jats:italic toggle=\"yes\">rv3400<\/jats:italic>\n                      also causes a growth defect when trehalose is the sole carbon source. Targeted metabolomics revealed that metabolites associated with redox homeostasis, including ergothioneine, mycothiol, and mycothione, are decreased in the Mtb \u0394\n                      <jats:italic toggle=\"yes\">rv3400<\/jats:italic>\n                      strain compared with the wild type, indicating altered tolerance to redox stress. Consistent with this, the \u0394\n                      <jats:italic toggle=\"yes\">rv3400<\/jats:italic>\n                      strain showed increased susceptibility to oxidative stress induced by H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      and cumene hydroperoxide, conditions that Mtb encounters during infection. This work advances our understanding of trehalose metabolism in Mtb and suggests that Rv3400 may represent a target for the development of new antimicrobial therapies.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      Trehalose metabolism plays a cornerstone role in\n                      <jats:italic toggle=\"yes\">Mycobacterium tuberculosis<\/jats:italic>\n                      physiology and virulence. A better understanding of the metabolism of this essential disaccharide is therefore required to develop new strategies to eradicate tuberculosis. Here, we characterize\n                      <jats:italic toggle=\"yes\">M. tuberculosis<\/jats:italic>\n                      lacking the \u03b2-phosphoglucomutase encoded by\n                      <jats:italic toggle=\"yes\">rv3400<\/jats:italic>\n                      . We show that deletion of\n                      <jats:italic toggle=\"yes\">rv3400<\/jats:italic>\n                      leads to accumulation of \u03b2-D-glucose-1-phosphate, impaired growth when trehalose is used as the sole carbon source, and increased susceptibility to oxidative stress. Taken together, these data provide evidence that Rv3400 is required for optimal catabolism of trehalose.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/jb.00343-26","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T14:10:12Z","timestamp":1788444612000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Rv3400 is a phosphoglucomutase required for trehalose metabolism in\n                    <i>Mycobacterium tuberculosis<\/i>"],"prefix":"10.1128","author":[{"given":"Yi","family":"Liu","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/041kmwe10","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Life Sciences, Faculty of Natural Sciences, Centre for Bacterial Resistance Biology, Imperial College London","place":["London, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadine","family":"Ruecker","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02r109517","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, Weill Cornell Medicine","place":["New York, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valwynne","family":"Faulkner","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04tnbqb63","id-type":"ROR","asserted-by":"publisher"}],"name":"Systems Chemical Biology of Infection and Resistance Laboratory, Francis Crick Institute","place":["London, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kavitha","family":"Rachineni","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/041kmwe10","id-type":"ROR","asserted-by":"publisher"}],"name":"Section of Bioanalytical Chemistry, Division of Systems Medicine, Department of Metabolism, Digestion and Reproduction, National Phenome Centre, Imperial College London","place":["London, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nada","family":"Al-Saffar","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/041kmwe10","id-type":"ROR","asserted-by":"publisher"}],"name":"Section of Bioanalytical Chemistry, Division of Systems Medicine, Department of Metabolism, Digestion and Reproduction, National Phenome Centre, Imperial College London","place":["London, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Oskoui","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/041kmwe10","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Life Sciences, Faculty of Natural Sciences, Centre for Bacterial Resistance Biology, Imperial College London","place":["London, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa Zarate","family":"Garcia","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/041kmwe10","id-type":"ROR","asserted-by":"publisher"}],"name":"Flow Cytometry Facility, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London","place":["London, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica Emma","family":"Rowley","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/041kmwe10","id-type":"ROR","asserted-by":"publisher"}],"name":"Flow Cytometry Facility, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London","place":["London, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo Villacampa","family":"Teixeira","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/041kmwe10","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Life Sciences, Faculty of Natural Sciences, Centre for Bacterial Resistance Biology, Imperial College London","place":["London, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiago R. 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Lipids of clinically significant mycobacteria, p 1\u201376. In Goldfine H (ed), Lipids of clinically significant mycobacteriahealth consequences of microbial interactions with hydrocarbons, oils, and lipids. 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