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In this study, we found that the<jats:italic>lox<\/jats:italic>mutant of<jats:italic>S. oligofermentans<\/jats:italic>regained the inhibition while growing on peptone-rich plates. Further studies demonstrated that the H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>produced on peptone by<jats:italic>S. oligofermentans<\/jats:italic>was mainly derived from seven<jats:sc>l<\/jats:sc>-amino acids, i.e.,<jats:sc>l<\/jats:sc>-aspartic acid,<jats:sc>l<\/jats:sc>-tryptophan,<jats:sc>l<\/jats:sc>-lysine,<jats:sc>l<\/jats:sc>-isoleucine,<jats:sc>l<\/jats:sc>-arginine,<jats:sc>l<\/jats:sc>-asparagine, and<jats:sc>l<\/jats:sc>-glutamine, indicating the possible existence of<jats:sc>l<\/jats:sc>-amino acid oxidase (LAAO) that can produce H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>from<jats:sc>l<\/jats:sc>-amino acids. Through searching the<jats:italic>S. oligofermentans<\/jats:italic>genome for open reading frames with a conserved flavin adenine dinucleotide binding motif that exists in the known LAAOs, including those of snake venom, fungi, and bacteria, a putative LAAO gene, assigned as<jats:italic>aao<jats:sub>S<\/jats:sub><\/jats:italic><jats:italic><jats:sub>o<\/jats:sub><\/jats:italic>, was cloned and overexpressed in<jats:italic>Escherichia coli<\/jats:italic>. The purified protein, SO-LAAO, showed a molecular mass of 43 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and catalyzed H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>formation from the seven<jats:sc>l<\/jats:sc>-amino acids determined above, thus confirming its LAAO activity. The SO-LAAO identified in<jats:italic>S. oligofermentans<\/jats:italic>differed evidently from the known LAAOs in both substrate profile and sequence, suggesting that it could represent a novel LAAO. An<jats:italic>aao<jats:sub>S<\/jats:sub><\/jats:italic><jats:italic><jats:sub>o<\/jats:sub><\/jats:italic>mutant of<jats:italic>S. oligofermentans<\/jats:italic>did lose H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>formation from the seven<jats:sc>l<\/jats:sc>-amino acids, further verifying its function as an LAAO. Furthermore, the inhibition by<jats:italic>S. oligofermentans<\/jats:italic>of<jats:italic>S. mutans<\/jats:italic>in a peptone-rich mixed-species biofilm was greatly reduced for the<jats:italic>aao<jats:sub>S<\/jats:sub><\/jats:italic><jats:italic><jats:sub>o<\/jats:sub><\/jats:italic>mutant, indicating the gene's importance in interspecies competition.<\/jats:p>","DOI":"10.1128\/jb.00363-08","type":"journal-article","created":{"date-parts":[[2008,5,10]],"date-time":"2008-05-10T00:54:27Z","timestamp":1210380867000},"page":"4716-4721","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":96,"title":["SO-LAAO, a Novel<scp>l<\/scp>-Amino Acid Oxidase That Enables<i>Streptococcus oligofermentans<\/i>To Outcompete<i>Streptococcus mutans<\/i>by Generating H<sub>2<\/sub>O<sub>2<\/sub>from Peptone"],"prefix":"10.1128","volume":"190","author":[{"given":"Huichun","family":"Tong","sequence":"first","affiliation":[{"name":"State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyuan","family":"Shi","sequence":"additional","affiliation":[{"name":"School of Dentistry, University of California, Los Angeles, California 90095"},{"name":"Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, California 90095"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengxia","family":"Qi","sequence":"additional","affiliation":[{"name":"Oral Biology, College of Dentistry, and Microbiology and Immunology, College of Medicine, University of Oklahoma Health Sciences Center BRC366, Oklahoma City, Oklahoma 73104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuzhu","family":"Dong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1007\/BF02976576","volume":"23","year":"2000","unstructured":"Ahn, M. 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