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Here, we report the development of a transposon mutagenesis system for\n            <jats:named-content content-type=\"genus-species\">C. muridarum<\/jats:named-content>\n            , a mouse-adapted\n            <jats:italic>Chlamydia<\/jats:italic>\n            species that is widely used for\n            <jats:italic>in vivo<\/jats:italic>\n            investigations of chlamydial pathogenesis. This advance builds on the pioneering development of this system for\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            . We demonstrate the generation of an initial library of 33 mutants containing stable single or double transposon insertions. Using these mutant clones, we characterized\n            <jats:italic>in vitro<\/jats:italic>\n            phenotypes associated with genetic disruptions in glycogen biosynthesis and three polymorphic outer membrane proteins.\n          <\/jats:p>","DOI":"10.1128\/jb.00366-19","type":"journal-article","created":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T12:41:36Z","timestamp":1568032896000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Development of Transposon Mutagenesis for Chlamydia muridarum"],"prefix":"10.1128","volume":"201","author":[{"given":"Yibing","family":"Wang","sequence":"first","affiliation":[{"name":"Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott D.","family":"LaBrie","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven J.","family":"Carrell","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Oregon State University, Corvallis, Oregon, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert J.","family":"Suchland","sequence":"additional","affiliation":[{"name":"Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoe E.","family":"Dimond","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Forrest","family":"Kwong","sequence":"additional","affiliation":[{"name":"Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel D.","family":"Rockey","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Oregon State University, Corvallis, Oregon, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. 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