{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T08:01:11Z","timestamp":1792310471458,"version":"4.4.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"20","license":[{"start":{"date-parts":[[2007,10,15]],"date-time":"2007-10-15T00:00:00Z","timestamp":1192406400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2007,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    We fully annotated two large plasmids, pMOL28 (164 open reading frames [ORFs]; 171,459 bp) and pMOL30 (247 ORFs; 233,720 bp), in the genome of\n                    <jats:italic>Cupriavidus metallidurans<\/jats:italic>\n                    CH34. pMOL28 contains a backbone of maintenance and transfer genes resembling those found in plasmid pSym of\n                    <jats:italic>C. taiwanensis<\/jats:italic>\n                    and plasmid pHG1 of\n                    <jats:italic>C. eutrophus<\/jats:italic>\n                    , suggesting that they belong to a new class of plasmids. Genes involved in resistance to the heavy metals Co(II), Cr(VI), Hg(II), and Ni(II) are concentrated in a 34-kb region on pMOL28, and genes involved in resistance to Ag(I), Cd(II), Co(II), Cu(II), Hg(II), Pb(II), and Zn(II) occur in a 132-kb region on pMOL30. We identified three putative genomic islands containing metal resistance operons flanked by mobile genetic elements, one on pMOL28 and two on pMOL30. Transcriptomic analysis using quantitative PCR and microarrays revealed metal-mediated up-regulation of 83 genes on pMOL28 and 143 genes on pMOL30 that coded for all known heavy metal resistance proteins, some new heavy metal resistance proteins (\n                    <jats:italic>czcJ<\/jats:italic>\n                    ,\n                    <jats:italic>mmrQ<\/jats:italic>\n                    , and\n                    <jats:italic>pbrU<\/jats:italic>\n                    ), membrane proteins, truncated transposases, conjugative transfer proteins, and many unknown proteins. Five genes on each plasmid were down-regulated; for one of them,\n                    <jats:italic>chrI<\/jats:italic>\n                    localized on pMOL28, the down-regulation occurred in the presence of five cations. We observed multiple cross-responses (induction of specific metal resistance by other metals), suggesting that the cellular defense of\n                    <jats:italic>C. metallidurans<\/jats:italic>\n                    against heavy metal stress involves various regulons and probably has multiple stages, including a more general response and a more metal-specific response.\n                  <\/jats:p>","DOI":"10.1128\/jb.00375-07","type":"journal-article","created":{"date-parts":[[2007,8,3]],"date-time":"2007-08-03T21:10:25Z","timestamp":1186175425000},"page":"7417-7425","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":238,"title":["Plasmids pMOL28 and pMOL30 of\n                    <i>Cupriavidus metallidurans<\/i>\n                    Are Specialized in the Maximal Viable Response to Heavy Metals"],"prefix":"10.1128","volume":"189","author":[{"given":"Se\u0301bastien","family":"Monchy","sequence":"first","affiliation":[{"name":"Molecular &amp; Cellular Biology, Institute for Health, Environment &amp; Safety, SCK \u00b7 CEN, Boeretang 200, B-2400 Mol, Belgium"},{"name":"Service de Conformation des Macromole\u0301cules Biologique et de Bioinformatique, Universite\u0301 Libre de Bruxelles, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed A.","family":"Benotmane","sequence":"additional","affiliation":[{"name":"Molecular &amp; Cellular Biology, Institute for Health, Environment &amp; Safety, SCK \u00b7 CEN, Boeretang 200, B-2400 Mol, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Janssen","sequence":"additional","affiliation":[{"name":"Molecular &amp; Cellular Biology, Institute for Health, Environment &amp; Safety, SCK \u00b7 CEN, Boeretang 200, B-2400 Mol, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana","family":"Vallaeys","sequence":"additional","affiliation":[{"name":"INRA, De\u0301partement de Microbiologie, F-78850 Thiverval Grignon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Safiyh","family":"Taghavi","sequence":"additional","affiliation":[{"name":"Brookhaven National Laboratory, Biology Department, Upton, New York 11973-5000"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"van der Lelie","sequence":"additional","affiliation":[{"name":"Brookhaven National Laboratory, Biology Department, Upton, New York 11973-5000"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Max","family":"Mergeay","sequence":"additional","affiliation":[{"name":"Molecular &amp; Cellular Biology, Institute for Health, Environment &amp; Safety, SCK \u00b7 CEN, Boeretang 200, B-2400 Mol, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.19.5651-5658.2001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1016\/S0723-2020(99)80073-3","volume":"22","year":"1999","unstructured":"Brim, H., M. 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