{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T23:14:46Z","timestamp":1790291686847,"version":"4.1.0"},"reference-count":68,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T00:00:00Z","timestamp":1776902400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T00:00:00Z","timestamp":1776902400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI172335"],"award-info":[{"award-number":["AI172335"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM156848"],"award-info":[{"award-number":["GM156848"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2026,4,23]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                      is a highly versatile bacterium capable of surviving and often thriving in stressful environmental conditions. Here, we report the effect of two environmental conditions, temperature and growth phase, on the\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      PAO1 transcriptome. As\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      is well-known for its growth phase dependent phenotypes and gene regulation, our goal was to determine how temperature altered global gene expression at exponential versus stationary phase and to characterize how growth phase affects thermoregulation. To do this, we grew PAO1 in parallel at 25\u00b0C and 37\u00b0C and sampled the same populations first at exponential phase and then again at stationary phase and assessed gene expression by RNA-sequencing. We found that temperature regulated hundreds of genes at, and unique to, exponential and stationary phases. We also grew PAO1 and an isogenic \u0394\n                      <jats:italic toggle=\"yes\">lasR<\/jats:italic>\n                      mutant at 25\u00b0C and 37\u00b0C and sampled populations at stationary phase to define LasR-regulated genes at each temperature by RNA-sequencing. LasR regulated most of its target genes similarly at 25\u00b0C and 37\u00b0C, although we identified a subset of genes whose regulation by LasR was affected by temperature. This work provides a comprehensive assessment of thermoregulation for PAO1 at two distinct growth phases, as well as growth phase transcriptomics at two temperatures, and expands our understanding of quorum sensing regulation under different environmental conditions that\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      encounters.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                      is a highly adaptable opportunistic pathogen with a repertoire of mechanisms for surviving in diverse and often challenging environments, yet it is most studied at 37\u00b0C as the optimum temperature for growth. To better understand how this bacterium survives in the environment versus the human body, we performed transcriptomics on\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      grown at 25\u00b0C and 37\u00b0C. At each temperature, we examined both exponential and stationary phases and determined the LasRI quorum sensing regulon at 37\u00b0C compared to 25\u00b0C using a \u0394\n                      <jats:italic toggle=\"yes\">lasR<\/jats:italic>\n                      mutant, which uncovered a suite of previously unrecognized LasR-regulated genes. Our work provides a comprehensive transcriptomic resource for the thermoregulation of\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      at two growth phases, as well as growth phase and LasR regulation at two temperatures.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/jb.00385-25","type":"journal-article","created":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T13:02:10Z","timestamp":1773234130000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["The interplay between temperature and growth phase shapes the transcriptional landscape of\n                    <i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.1128","volume":"208","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7493-942X","authenticated-orcid":false,"given":"Rachel E.","family":"Robinson","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03czfpz43","id-type":"ROR","asserted-by":"publisher"}],"name":"Microbiology and Molecular Genetics Program, Graduate Division of Biological and Biomedical Sciences, Laney Graduate School, Emory University","place":["Atlanta, USA"]},{"id":[{"id":"https:\/\/ror.org\/02gars961","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pediatrics, Division of Pulmonary, Asthma, Cystic Fibrosis, and Sleep, Emory University School of Medicine","place":["Atlanta, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1213-8408","authenticated-orcid":true,"given":"Michael J.","family":"Gebhardt","sequence":"additional","affiliation":[{"name":"Department of Microbiology & Immunology, Carver College of Medicine, University of Iowa","place":["Iowa City, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5285-5188","authenticated-orcid":true,"given":"Joanna B.","family":"Goldberg","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02gars961","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pediatrics, Division of Pulmonary, Asthma, Cystic Fibrosis, and Sleep, Emory University School of Medicine","place":["Atlanta, USA"]},{"id":[{"id":"https:\/\/ror.org\/02gars961","id-type":"ROR","asserted-by":"publisher"}],"name":"Emory+Children\u2019s Center for Cystic Fibrosis and Airway Disease Research, Emory University School of Medicine","place":["Atlanta, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-025-01193-8"},{"key":"e_1_3_4_3_2","unstructured":"Cystic Fibrosis Foundation. 2023. 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