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Here, we report that LasR\u2212 strains are more sensitive to the electrophile methylglyoxal (MGO), which is produced by many cells, including those involved in immune defense. MGO, due to its high reactivity, must be rapidly detoxified to prevent the formation of damaging adducts on macromolecules. GloA3 is a GSH-dependent MGO glyoxalase involved in MGO detoxification, and we found that intracellular GSH levels were lower in \u2206\n                      <jats:italic toggle=\"yes\">lasR<\/jats:italic>\n                      strains, as measured by multiple methods. Furthermore, we show that exogenous GSH and GloA3 overexpression both enhance GloA3-dependent MGO resistance, particularly in a \u2206\n                      <jats:italic toggle=\"yes\">lasR<\/jats:italic>\n                      mutant. Previous reports suggest that one factor that contributes to the selection for LasR\u2212 strains is a growth advantage resulting from increased activity of the CbrAB two-component system, which lowers the Crc-mediated translational repression of many RNAs. Genetic data presented here suggest that low Crc activity leads to increased MGO sensitivity in LasR\u2212 strains but not through effects on GSH or GloA3. Together, these data suggest that LasR\u2212 strains are more sensitive to MGO due to multiple factors, including lower intracellular levels of GSH and decreased Crc activity, and that these factors may represent fitness trade-offs for LasR\u2212 strains.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Methylglyoxal (MGO) is a highly reactive metabolite detected in various disease states, including those involving\n                        <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                        .\n                        <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                        requires the glutathione-dependent glyoxalase enzyme GloA3 for MGO resistance. This study reveals that\n                        <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                        strains with mutations in the gene encoding the transcription factor LasR, commonly found in clinical isolates, are more sensitive to MGO due to lower intracellular glutathione levels and high activity of the CbrAB-Crc regulatory pathway. Thus, sensitivity to MGO and other electrophiles may represent a trade-off for\n                        <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                        in infections.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/jb.00394-24","type":"journal-article","created":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T13:21:05Z","timestamp":1758201665000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Effects of low intracellular glutathione and CbrA-CbrB-Crc signaling on methylglyoxal sensitivity in\n                    <i>Pseudomonas aeruginosa<\/i>\n                    LasR-deficient mutants"],"prefix":"10.1128","volume":"207","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7866-0269","authenticated-orcid":true,"given":"Ana","family":"Altamirano","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/049s0rh22","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth","place":["Hanover, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Ruzic","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/049s0rh22","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth","place":["Hanover, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna E.","family":"Cryan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/049s0rh22","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and 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