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Defective entry and phagosome acidification phenotypes of\n            <jats:italic>dot<\/jats:italic>\n            \/\n            <jats:italic>icm<\/jats:italic>\n            mutants are conditional and reversed by incubating broth-grown stationary-phase cultures in water (WS treatment) prior to infection, as a mimic of the aquatic environment of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Legionella<\/jats:named-content>\n            . Reversal of\n            <jats:italic>dot<\/jats:italic>\n            \/\n            <jats:italic>icm<\/jats:italic>\n            virulence defects requires the Lvh T4SS and is associated with a &gt;10-fold induction of LpnE, a tetratricopeptide repeat (TPR)-containing protein. In the current study, we demonstrated that defective entry and phagosome acidification phenotypes of mutants with changes in LpnE and EnhC, another TPR-containing protein, were similarly reversed by WS treatment. In contrast to\n            <jats:italic>dot<\/jats:italic>\n            \/\n            <jats:italic>icm<\/jats:italic>\n            mutants for which the Lvh T4SS was required, reversal for the \u0394\n            <jats:italic>lpnE<\/jats:italic>\n            or the \u0394\n            <jats:italic>enhC<\/jats:italic>\n            mutant required that the other TPR-containing protein be present. The single and double \u0394\n            <jats:italic>lpnE<\/jats:italic>\n            and \u0394\n            <jats:italic>enhC<\/jats:italic>\n            mutants showed a hypersensitivity to sodium ion, a phenotype associated with dysfunction of the Dot\/Icm T4SS. The \u0394\n            <jats:italic>lpnE<\/jats:italic>\n            single and the \u0394\n            <jats:italic>lpnE<\/jats:italic>\n            \u0394\n            <jats:italic>enhC<\/jats:italic>\n            double mutant showed 3- to 9-fold increases in translocation of Dot\/Icm T4SS substrates, LegS2\/SplY and LepB. Taken together, these data identify TPR-containing proteins in a second mechanism by which the WS mimic of a\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Legionella<\/jats:named-content>\n            environmental niche can reverse virulence defects of broth-grown cultures and implicate LpnE and EnhC directly or indirectly in translocation of Dot\/Icm T4SS protein substrates.\n          <\/jats:p>","DOI":"10.1128\/jb.00399-12","type":"journal-article","created":{"date-parts":[[2012,5,5]],"date-time":"2012-05-05T12:33:33Z","timestamp":1336221213000},"page":"3579-3588","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Implication of Proteins Containing Tetratricopeptide Repeats in Conditional Virulence Phenotypes of Legionella pneumophila"],"prefix":"10.1128","volume":"194","author":[{"given":"Purnima","family":"Bandyopadhyay","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eren U.","family":"Sumer","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepak","family":"Jayakumar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuqing","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huifang","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Howard M.","family":"Steinman","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.23.6679-6686.2000"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00956-06"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.8.4541-4551.2004"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.8.4526-4535.2003"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1111\/j.1365-2958.2005.04539.x","article-title":"IcmS-dependent translocation of SdeA into macrophages by the Legionella pneumophila type IV secretion system","volume":"56","author":"Bardill JP","year":"2005","unstructured":"BardillJP MillerJL VogelJP. 2005. 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