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The culture conditions for effector production in\n                    <jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>\n                    have not been investigated. We attempted to optimize culture medium compositions for producing and secreting type III secreted proteins. We found that\n                    <jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>\n                    secretes type III secreted proteins in reducing agent-deprived liquid medium and that BteA-secreting\n                    <jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>\n                    provokes cytotoxicity against cultured mammalian cells. These results suggest that redox signaling is involved in the regulation of\n                    <jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>\n                    T3SS.\n                  <\/jats:p>","DOI":"10.1128\/jb.00400-20","type":"journal-article","created":{"date-parts":[[2020,8,12]],"date-time":"2020-08-12T08:54:24Z","timestamp":1597222464000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Transcriptional Downregulation of a Type III Secretion System under Reducing Conditions in Bordetella pertussis"],"prefix":"10.1128","volume":"202","author":[{"given":"Masataka","family":"Goto","sequence":"first","affiliation":[{"name":"Laboratory of Bacterial Infection, Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoko","family":"Hanawa","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Kyorin University School of Medicine, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akio","family":"Abe","sequence":"additional","affiliation":[{"name":"Laboratory of Bacterial Infection, Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1311-1119","authenticated-orcid":false,"given":"Asaomi","family":"Kuwae","sequence":"additional","affiliation":[{"name":"Laboratory of Bacterial Infection, Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.001540"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.2.326-382.2005"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.8.2637"},{"key":"e_1_3_3_5_2","doi-asserted-by":"crossref","first-page":"19379","DOI":"10.1016\/S0021-9258(19)47312-2","article-title":"Adenylate cyclase toxin from Bordetella pertussis. 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