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Like other diderm bacteria,\n                      <jats:italic toggle=\"yes\">Caulobacter crescentus<\/jats:italic>\n                      can acquire Fe(III) via outer-membrane TonB-dependent transporters (TBDTs) that recognize and import ferric siderophore complexes. Although\n                      <jats:italic toggle=\"yes\">C. crescentus<\/jats:italic>\n                      is not known to synthesize siderophores, it encodes multiple TBDTs that are transcriptionally regulated by the ferric uptake repressor (Fur), suggesting it acquires iron by scavenging xenosiderophores produced by neighboring microbes. To identify\n                      <jats:italic toggle=\"yes\">C. crescentus<\/jats:italic>\n                      genes required for xenosiderophore utilization, we developed a barcoded transposon screen using ferrioxamine B (FXB), a hydroxamate-family siderophore produced by soil actinomycetes, as a model substrate. This screen identified\n                      <jats:italic toggle=\"yes\">hiuABC<\/jats:italic>\n                      , a conserved, Fur-regulated operon that supports FXB-dependent iron acquisition. We provide evidence that\n                      <jats:italic toggle=\"yes\">hiuA<\/jats:italic>\n                      encodes the primary TBDT responsible for uptake of ferrioxamines and ferrichrome (FC), structurally distinct members of the hydroxamate siderophore family.\n                      <jats:italic toggle=\"yes\">hiuB<\/jats:italic>\n                      encodes a PepSY-domain protein with structural similarity to\n                      <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                      FoxB, a known periplasmic ferri-siderophore reductase.\n                      <jats:italic toggle=\"yes\">hiuC<\/jats:italic>\n                      encodes a small, hypothetical membrane protein predicted to form a functional complex with HiuB in the inner membrane. Both\n                      <jats:italic toggle=\"yes\">hiuB<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">hiuC<\/jats:italic>\n                      are required for utilization of FXB and ferrioxamine E, indicating a shared role in iron acquisition from ferrioxamines. Surprisingly, utilization of FC as an iron source required\n                      <jats:italic toggle=\"yes\">hiuB<\/jats:italic>\n                      but not\n                      <jats:italic toggle=\"yes\">hiuC<\/jats:italic>\n                      , suggesting a substrate-specific role for HiuC in ferri-siderophore processing. We conclude that the conserved\n                      <jats:italic toggle=\"yes\">hiuABC<\/jats:italic>\n                      operon encodes a set of proteins that enable bacteria to acquire iron from structurally diverse hydroxamate-family siderophores.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Iron is often a limiting nutrient due to its poor solubility in the presence of oxygen. To overcome this, some microbes produce specialized molecules known as siderophores, which tightly bind and solubilize iron, facilitating its uptake into the cell.\n                        <jats:italic toggle=\"yes\">Caulobacter<\/jats:italic>\n                        species are common in freshwater, marine, and soil environments, and there is emerging evidence that they play important roles in plant-associated microbial communities. Here, we report the discovery of a three-gene system that allows\n                        <jats:italic toggle=\"yes\">Caulobacter crescentus<\/jats:italic>\n                        to acquire iron from a set of siderophores produced by select soil bacteria and fungi. We define functional roles for each protein component of this system, which informs a mechanism by which\n                        <jats:italic toggle=\"yes\">Caulobacter<\/jats:italic>\n                        can pirate iron-scavenging molecules produced by its neighbors.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/jb.00400-25","type":"journal-article","created":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T14:00:56Z","timestamp":1770213656000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["The\n                    <i>hiuABC<\/i>\n                    operon mediates xenosiderophore utilization in\n                    <i>Caulobacter crescentus<\/i>"],"prefix":"10.1128","volume":"208","author":[{"given":"Sergio","family":"Hernandez-Ortiz","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05hs6h993","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, Genetics, and Immunology, Michigan State University","place":["East Lansing, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0612-5029","authenticated-orcid":true,"given":"Aretha","family":"Fiebig","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05hs6h993","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, Genetics, and Immunology, Michigan State University","place":["East Lansing, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1727-322X","authenticated-orcid":true,"given":"Sean","family":"Crosson","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05hs6h993","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, Genetics, and Immunology, Michigan State University","place":["East Lansing, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.48.100194.003523"},{"key":"e_1_3_3_3_2","doi-asserted-by":"crossref","unstructured":"Clarke FW Washington HS. 1924. 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