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The multidrug efflux pump MtrCDE transports multiple drugs and host-derived antimicrobials from the bacterial cell and confers survival advantage on the pathogen within the host. Transcription of the pump is repressed by MtrR but relieved by the cytosolic influx of antimicrobials. Here, we describe the structure of induced MtrR and use this structure to identify bile salts as physiological inducers of MtrR. These findings provide a mechanistic basis for antimicrobial sensing and gonococcal protection by MtrR through the derepression of\n            <jats:italic>mtrCDE<\/jats:italic>\n            expression after exposure to intrinsic and clinically applied antimicrobials.\n          <\/jats:p>","DOI":"10.1128\/jb.00401-19","type":"journal-article","created":{"date-parts":[[2019,7,23]],"date-time":"2019-07-23T10:50:22Z","timestamp":1563879022000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Structural, Biochemical, and\n            <i>In Vivo<\/i>\n            Characterization of MtrR-Mediated Resistance to Innate Antimicrobials by the Human Pathogen\n            <i>Neisseria gonorrhoeae<\/i>"],"prefix":"10.1128","volume":"201","author":[{"given":"Grace A.","family":"Beggs","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Duke University School of Medicine, Durham, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaramah M.","family":"Zalucki","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia, USA"},{"name":"Emory Antibiotic Resistance Center, Emory University School of Medicine, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas Gene","family":"Brown","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Chemical Biology, Baylor College of Medicine, Houston, Texas, USA"},{"name":"Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheila","family":"Rastegari","sequence":"additional","affiliation":[{"name":"Center for Molecular and Translational Human Infectious Diseases Research, Houston Methodist Hospital Research Institute, Houston, Texas, USA"},{"name":"Department of Biochemistry and Molecular Biology, University of Texas, MD Anderson Cancer Center, Houston, Texas, USA"},{"name":"Department of Pathology and Genomic Medicine, Houston Methodist Hospital System, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca K.","family":"Phillips","sequence":"additional","affiliation":[{"name":"Center for Molecular and Translational Human Infectious Diseases Research, Houston Methodist Hospital Research Institute, Houston, Texas, USA"},{"name":"Department of Pathology and Genomic Medicine, Houston Methodist Hospital System, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"Palzkill","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Chemical Biology, Baylor College of Medicine, Houston, Texas, USA"},{"name":"Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William M.","family":"Shafer","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia, USA"},{"name":"Laboratories of Microbial Pathogenesis, VA Medical Research Service, Veterans Affairs Medical Center, Decatur, Georgia, USA"},{"name":"Emory Antibiotic Resistance Center, Emory University School of Medicine, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9959-5598","authenticated-orcid":false,"given":"Muthiah","family":"Kumaraswami","sequence":"additional","affiliation":[{"name":"Center for Molecular and Translational Human Infectious Diseases Research, Houston Methodist Hospital Research Institute, Houston, Texas, USA"},{"name":"Department of Pathology and Genomic Medicine, Houston Methodist Hospital System, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7647-485X","authenticated-orcid":false,"given":"Richard G.","family":"Brennan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Duke University School of Medicine, Durham, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.EI10-0009-2015"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1002344"},{"key":"e_1_3_3_4_2","first-page":"332","article-title":"Sexually transmitted diseases treatment guidelines","volume":"56","author":"Workowski KA","year":"2007","unstructured":"Workowski KA, Berman SM. 2007. 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