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In many organisms, there is more than one\n            <jats:italic>amt<\/jats:italic>\n            gene, and these genes are subjected to nitrogen control. The mature Amt protein is a homo- or heterooligomer of three Amt subunits. We previously characterized an\n            <jats:italic>amt1<\/jats:italic>\n            gene in the unicellular cyanobacterium\n            <jats:italic>Synechococcus elongatus<\/jats:italic>\n            strain PCC 7942. In this work, we describe the presence in this organism of a second\n            <jats:italic>amt<\/jats:italic>\n            gene,\n            <jats:italic>amtB<\/jats:italic>\n            , which encodes a protein more similar to the bacterial AmtB proteins than to any other characterized cyanobacterial Amt protein. The expression of\n            <jats:italic>amtB<\/jats:italic>\n            took place in response to nitrogen step-down, required the NtcA transcription factor, and occurred parallel to the expression of\n            <jats:italic>amt1<\/jats:italic>\n            . However, the transcript levels of\n            <jats:italic>amtB<\/jats:italic>\n            measured after 2 h of nitrogen deprivation were about 100-fold lower than those of\n            <jats:italic>amt1<\/jats:italic>\n            . An\n            <jats:italic>S. elongatus amtB<\/jats:italic>\n            insertional mutant exhibited an activity for uptake of [\n            <jats:sup>14<\/jats:sup>\n            C]methylammonium that was about 55% of that observed in the wild type, but inactivation of\n            <jats:italic>amtB<\/jats:italic>\n            had no noticeable effect on the uptake of ammonium when it was supplied at a concentration of 100 \u03bcM or more. Because an\n            <jats:italic>S. elongatus amt1<\/jats:italic>\n            mutant is essentially devoid of [\n            <jats:sup>14<\/jats:sup>\n            C]methylammonium uptake activity, the mature Amt transporter is functional in the absence of AmtB subunits but not in the absence of Amt1 subunits. However, the\n            <jats:italic>S. elongatus amtB<\/jats:italic>\n            mutant could not concentrate [\n            <jats:sup>14<\/jats:sup>\n            C]methylammonium within the cells to the same extent as the wild type. Therefore, AmtB is necessary for full methylammonium uptake activity in\n            <jats:italic>S. elongatus<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jb.00404-07","type":"journal-article","created":{"date-parts":[[2007,8,18]],"date-time":"2007-08-18T00:53:49Z","timestamp":1187398429000},"page":"7791-7798","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["The NtcA-Regulated\n            <i>amtB<\/i>\n            Gene Is Necessary for Full Methylammonium Uptake Activity in the Cyanobacterium\n            <i>Synechococcus elongatus<\/i>"],"prefix":"10.1128","volume":"189","author":[{"given":"Javier","family":"Paz-Yepes","sequence":"first","affiliation":[{"name":"Instituto de Bioqui\u0301mica Vegetal y Fotosi\u0301ntesis, Consejo Superior de Investigaciones Cienti\u0301ficas and Universidad de Sevilla, E-41092 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonia","family":"Herrero","sequence":"additional","affiliation":[{"name":"Instituto de Bioqui\u0301mica Vegetal y Fotosi\u0301ntesis, Consejo Superior de Investigaciones Cienti\u0301ficas and Universidad de Sevilla, E-41092 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrique","family":"Flores","sequence":"additional","affiliation":[{"name":"Instituto de Bioqui\u0301mica Vegetal y Fotosi\u0301ntesis, Consejo Superior de Investigaciones Cienti\u0301ficas and Universidad de Sevilla, E-41092 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"14994","DOI":"10.1073\/pnas.0506254102","volume":"102","year":"2005","unstructured":"Andrade, S. 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