{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T02:28:38Z","timestamp":1792722518115,"version":"4.5.0"},"reference-count":67,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[2008,7,1]],"date-time":"2008-07-01T00:00:00Z","timestamp":1214870400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2008,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The<jats:italic>Brucella abortus virB<\/jats:italic>locus contains 12 open reading frames, termed<jats:italic>virB1<\/jats:italic>through<jats:italic>virB12<\/jats:italic>, which encode a type IV secretion system. Polar mutations in the<jats:italic>virB<\/jats:italic>locus markedly reduce the ability of<jats:italic>B. abortus<\/jats:italic>to survive in cultured macrophages or to persist in organs of mice. While a nonpolar deletion of the<jats:italic>virB2<\/jats:italic>gene reduces survival in cultured macrophages and in organs of mice, a nonpolar deletion of<jats:italic>virB1<\/jats:italic>only reduces survival in macrophages, whereas<jats:italic>virB12<\/jats:italic>is dispensable for either virulence trait. Here we investigated the role of the remaining genes in the<jats:italic>virB<\/jats:italic>locus during survival in macrophages and virulence in mice. Mutants carrying nonpolar deletions of the<jats:italic>virB3<\/jats:italic>,<jats:italic>virB4<\/jats:italic>,<jats:italic>virB5<\/jats:italic>,<jats:italic>virB6<\/jats:italic>,<jats:italic>virB7<\/jats:italic>,<jats:italic>virB8<\/jats:italic>,<jats:italic>virB9<\/jats:italic>,<jats:italic>virB10<\/jats:italic>, or<jats:italic>virB11<\/jats:italic>gene were constructed and characterized. All mutations reduced the ability of<jats:italic>B. abortus<\/jats:italic>to survive in J774A.1 mouse macrophage-like cells to a degree similar to that caused by a deletion of the entire<jats:italic>virB<\/jats:italic>locus. Deletion of<jats:italic>virB3<\/jats:italic>,<jats:italic>virB4<\/jats:italic>,<jats:italic>virB5<\/jats:italic>,<jats:italic>virB6<\/jats:italic>,<jats:italic>virB8<\/jats:italic>,<jats:italic>virB9<\/jats:italic>,<jats:italic>virB10<\/jats:italic>, or<jats:italic>virB11<\/jats:italic>markedly reduced the ability of<jats:italic>B. abortus<\/jats:italic>to persist in the spleens of mice at 8 weeks after infection. Interestingly, deletion of<jats:italic>virB7<\/jats:italic>did not reduce the ability of<jats:italic>B. abortus<\/jats:italic>to persist in spleens of mice. We conclude that<jats:italic>virB2<\/jats:italic>,<jats:italic>virB3<\/jats:italic>,<jats:italic>virB4<\/jats:italic>,<jats:italic>virB5<\/jats:italic>,<jats:italic>virB6<\/jats:italic>,<jats:italic>virB8<\/jats:italic>,<jats:italic>virB9<\/jats:italic>,<jats:italic>virB10<\/jats:italic>, and<jats:italic>virB11<\/jats:italic>are essential for virulence of<jats:italic>B. abortus<\/jats:italic>in mice, while functions encoded by the<jats:italic>virB1<\/jats:italic>,<jats:italic>virB7<\/jats:italic>, and<jats:italic>virB12<\/jats:italic>genes are not required for persistence in organs with this animal model.<\/jats:p>","DOI":"10.1128\/jb.00406-08","type":"journal-article","created":{"date-parts":[[2008,5,10]],"date-time":"2008-05-10T00:54:27Z","timestamp":1210380867000},"page":"4427-4436","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":83,"title":["VirB3 to VirB6 and VirB8 to VirB11, but Not VirB7, Are Essential for Mediating Persistence of<i>Brucella<\/i>in the Reticuloendothelial System"],"prefix":"10.1128","volume":"190","author":[{"given":"Andreas B.","family":"den Hartigh","sequence":"first","affiliation":[{"name":"University of California, Davis, Department of Medical Microbiology and Immunology, Davis, California 95616"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hortensia G.","family":"Rola\u0301n","sequence":"additional","affiliation":[{"name":"University of California, Davis, Department of Medical Microbiology and Immunology, Davis, California 95616"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maarten F.","family":"de Jong","sequence":"additional","affiliation":[{"name":"University of California, Davis, Department of Medical Microbiology and Immunology, Davis, California 95616"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rene\u0301e M.","family":"Tsolis","sequence":"additional","affiliation":[{"name":"University of California, Davis, Department of Medical Microbiology and Immunology, Davis, California 95616"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.3.950-958.1998"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1111\/j.1365-2958.2004.04345.x","volume":"54","year":"2004","unstructured":"Atmakuri, K., E. Cascales, and P. J. Christie. 2004. Energetic components VirD4, VirB11 and VirB4 mediate early DNA transfer reactions required for bacterial type IV secretion. Mol. Microbiol. 54 : 1199-1211.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_4_2","unstructured":"Current protocols in molecular biology. 1994"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1046\/j.1462-5822.2000.00043.x","volume":"2","year":"2000","unstructured":"Backert, S., E. Ziska, V. Brinkmann, U. Zimny-Arndt, A. Fauconnier, P. R. Jungblut, M. Naumann, and T. F. Meyer. 2000. Translocation of the Helicobacter pylori CagA protein in gastric epithelial cells by a type IV secretion apparatus. Cell Microbiol. 2 : 155-164.","journal-title":"Cell Microbiol."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.12.3646-3660.1994"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"1544","DOI":"10.1073\/pnas.032514299","volume":"99","year":"2002","unstructured":"Boschiroli, M. L., S. Ouahrani-Bettache, V. Foulongne, S. Michaux-Charachon, G. Bourg, A. Allardet-Servent, C. Cazevieille, J. P. Liautard, M. Ramuz, and D. O'Callaghan. 2002. The Brucella suis virB operon is induced intracellularly in macrophages. Proc. Natl. Acad. Sci. USA 99 : 1544-1549.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1073\/pnas.0406656102","volume":"102","year":"2005","unstructured":"Brayton, K. A., L. S. Kappmeyer, D. R. Herndon, M. J. Dark, D. L. Tibbals, G. H. Palmer, T. C. McGuire, and D. P. Knowles, Jr. 2005. Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins. Proc. Natl. Acad. Sci. USA 102 : 844-849.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1111\/j.1365-2958.2005.04595.x","volume":"56","year":"2005","unstructured":"Campodonico, E. M., L. Chesnel, and C. R. Roy. 2005. A yeast genetic system for the identification and characterization of substrate proteins transferred into host cells by the Legionella pneumophila Dot\/Icm system. Mol. Microbiol. 56 : 918-933.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-147-12-3201"},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1084\/jem.20030088","volume":"198","year":"2003","unstructured":"Celli, J., C. de Chastellier, D. M. Franchini, J. Pizarro-Cerda, E. Moreno, and J. P. Gorvel. 2003. Brucella evades macrophage killing via VirB-dependent sustained interactions with the endoplasmic reticulum. J. Exp. Med. 198 : 545-556.","journal-title":"J. Exp. Med."},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.1126\/science.1094226","volume":"303","year":"2004","unstructured":"Chen, J., K. S. de Felipe, M. Clarke, H. Lu, O. R. Anderson, G. Segal, and H. A. Shuman. 2004. Legionella effectors that promote nonlytic release from protozoa. Science 303 : 1358-1361.","journal-title":"Science"},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","first-page":"1660","DOI":"10.1111\/j.1462-5822.2007.00899.x","volume":"9","year":"2007","unstructured":"Chen, J., M. Reyes, M. Clarke, and H. A. Shuman. 2007. Host cell-dependent secretion and translocation of the LepA and LepB effectors of Legionella pneumophila. Cell Microbiol. 9 : 1660-1671.","journal-title":"Cell Microbiol."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.58.030603.123630"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(00)01792-3"},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1046\/j.1462-5822.2001.00102.x","volume":"3","year":"2001","unstructured":"Comerci, D. J., M. J. Martinez-Lorenzo, R. Sieira, J. P. Gorvel, and R. A. Ugalde. 2001. Essential role of the VirB machinery in the maturation of the Brucella abortus-containing vacuole. Cell Microbiol. 3 : 159-168.","journal-title":"Cell Microbiol."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03400.x"},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1111\/j.1574-6968.1999.tb08766.x","volume":"179","year":"1999","unstructured":"Craig-Mylius, K. A., and A. A. Weiss. 1999. Mutants in the ptlA-H genes of Bordetella pertussis are deficient for pertussis toxin secretion. FEMS Microbiol. Lett. 179 : 479-484.","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1046\/j.1462-5822.2001.00131.x","volume":"3","year":"2001","unstructured":"Delrue, R. M., M. Martinez-Lorenzo, P. Lestrate, I. Danese, V. Bielarz, P. Mertens, X. De Bolle, A. Tibor, J. P. Gorvel, and J. J. Letesson. 2001. Identification of Brucella spp. genes involved in intracellular trafficking. Cell Microbiol. 3 : 487-497.","journal-title":"Cell Microbiol."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.9.5143-5149.2004"},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1046\/j.1365-2958.2001.02520.x","volume":"41","year":"2001","unstructured":"Dillard, J. P., and H. S. Seifert. 2001. A variable genetic island specific for Neisseria gonorrhoeae is involved in providing DNA for natural transformation and is found more often in disseminated infection isolates. Mol. Microbiol. 41 : 263-277.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.11.3168-3176.1996"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.17.4881-4889.1995"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02714.x"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.3.1297-1303.2000"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msi124"},{"key":"e_1_3_2_27_2","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1051\/vetres:2005003","volume":"36","year":"2005","unstructured":"Godfroid, J., A. Cloeckaert, J. P. Liautard, S. Kohler, D. Fretin, K. Walravens, B. Garin-Bastuji, and J. J. Letesson. 2005. From the discovery of the Malta fever's agent to the discovery of a marine mammal reservoir, brucellosis has continuously been a re-emerging zoonosis. Vet. Res. 36 : 313-326.","journal-title":"Vet. Res."},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.16.4505-4511.2000"},{"key":"e_1_3_2_29_2","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0378-1119(98)00130-9","volume":"212","year":"1998","unstructured":"Hoang, T. T., R. R. Karkhoff-Schweizer, A. J. Kutchma, and H. P. Schweizer. 1998. A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants. Gene 212 : 77-86.","journal-title":"Gene"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.7.4102-4107.2000"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.9.2867-2878.2003"},{"key":"e_1_3_2_32_2","doi-asserted-by":"crossref","first-page":"3483","DOI":"10.1099\/mic.0.28337-0","volume":"151","year":"2005","unstructured":"Judd, P. K., D. Mahli, and A. Das. 2005. Molecular characterization of the Agrobacterium tumefaciens DNA transfer protein VirB6. Microbiology 151 : 3483-3492.","journal-title":"Microbiology"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.6.3020-3027.2003"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00584-1"},{"key":"e_1_3_2_35_2","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/S0923-2508(97)87645-0","volume":"148","year":"1997","unstructured":"Kulakov, Y. K., P. G. Guigue-Talet, M. R. Ramuz, and D. O'Callaghan. 1997. Response of Brucella suis 1330 and B. canis RM6\/66 to growth at acid pH and induction of an adaptive acid tolerance response. Res. Microbiol. 148 : 145-151.","journal-title":"Res. Microbiol."},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_3_2_37_2","doi-asserted-by":"crossref","first-page":"18745","DOI":"10.1073\/pnas.0609012103","volume":"103","year":"2006","unstructured":"Laguna, R. K., E. A. Creasey, Z. Li, N. Valtz, and R. R. Isberg. 2006. A Legionella pneumophila-translocated substrate that is required for growth within macrophages and protection from host cell death. Proc. Natl. Acad. Sci. USA 103 : 18745-18750.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_38_2","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1073\/pnas.0304916101","volume":"101","year":"2004","unstructured":"Luo, Z. Q., and R. R. Isberg. 2004. Multiple substrates of the Legionella pneumophila Dot\/Icm system identified by interbacterial protein transfer. Proc. Natl. Acad. Sci. USA 101 : 841-846.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2008.00472.x"},{"key":"e_1_3_2_40_2","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1038\/ncb1463","volume":"8","year":"2006","unstructured":"Murata, T., A. Delprato, A. Ingmundson, D. K. Toomre, D. G. Lambright, and C. R. Roy. 2006. The Legionella pneumophila effector protein DrrA is a Rab1 guanine nucleotide-exchange factor. Nat. Cell Biol. 8 : 971-977.","journal-title":"Nat. Cell Biol."},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1067025"},{"key":"e_1_3_2_42_2","doi-asserted-by":"crossref","first-page":"5962","DOI":"10.1093\/emboj\/20.21.5962","volume":"20","year":"2001","unstructured":"Nagai, H., and C. R. Roy. 2001. The DotA protein from Legionella pneumophila is secreted by a novel process that requires the Dot\/Icm transporter. EMBO J. 20 : 5962-5970.","journal-title":"EMBO J."},{"key":"e_1_3_2_43_2","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.1046\/j.1365-2958.1999.01569.x","volume":"33","year":"1999","unstructured":"O'Callaghan, D., C. Cazevieille, A. Allardet-Servent, M. L. Boschiroli, G. Bourg, V. Foulongne, P. Frutos, Y. Kulakov, and M. Ramuz. 1999. A homologue of the Agrobacterium tumefaciens VirB and Bordetella pertussis Ptl type IV secretion systems is essential for intracellular survival of Brucella suis. Mol. Microbiol. 33 : 1210-1220.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_44_2","doi-asserted-by":"crossref","first-page":"1497","DOI":"10.1126\/science.287.5457.1497","volume":"287","year":"2000","unstructured":"Odenbreit, S., J. Puls, B. Sedlmaier, E. Gerland, W. Fischer, and R. Haas. 2000. Translocation of Helicobacter pylori CagA into gastric epithelial cells by type IV secretion. Science 287 : 1497-1500.","journal-title":"Science"},{"key":"e_1_3_2_45_2","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/S1473-3099(06)70382-6","volume":"6","year":"2006","unstructured":"Pappas, G., P. Papadimitriou, N. Akritidis, L. Christou, and E. V. Tsianos. 2006. The new global map of human brucellosis. Lancet Infect. Dis. 6 : 91-99.","journal-title":"Lancet Infect. Dis."},{"key":"e_1_3_2_46_2","doi-asserted-by":"crossref","first-page":"7252","DOI":"10.1073\/pnas.0600862103","volume":"103","year":"2006","unstructured":"Paschos, A., G. Patey, D. Sivanesan, C. Gao, R. Bayliss, G. Waksman, D. O'Callaghan, and C. Baron. 2006. Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity. Proc. Natl. Acad. Sci. USA 103 : 7252-7257.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00584-06"},{"key":"e_1_3_2_48_2","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1038\/257393a0","volume":"257","year":"1975","unstructured":"Ralph, P., and I. Nakoinz. 1975. Phagocytosis and cytolysis by a macrophage tumour and its cloned cell line. Nature 257 : 393-394.","journal-title":"Nature"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01896-06"},{"key":"e_1_3_2_50_2","doi-asserted-by":"crossref","first-page":"1851","DOI":"10.1111\/j.1462-5822.2007.00922.x","volume":"9","year":"2007","unstructured":"Roux, C. M., H. G. Rolan, R. L. Santos, P. D. Beremand, T. L. Thomas, L. G. Adams, and R. M. Tsolis. 2007. Brucella requires a functional type IV secretion system to elicit innate immune responses in mice. Cell Microbiol. 9 : 1851-1869.","journal-title":"Cell Microbiol."},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.24.7485-7492.1999"},{"key":"e_1_3_2_52_2","doi-asserted-by":"crossref","first-page":"5169","DOI":"10.1093\/nar\/29.24.5169","volume":"29","year":"2001","unstructured":"Schneiker, S., M. Keller, M. Droge, E. Lanka, A. Puhler, and W. Selbitschka. 2001. The genetic organization and evolution of the broad host range mercury resistance plasmid pSB102 isolated from a microbial population residing in the rhizosphere of alfalfa. Nucleic Acids Res. 29 : 5169-5181.","journal-title":"Nucleic Acids Res."},{"key":"e_1_3_2_53_2","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1046\/j.1365-2958.2002.03208.x","volume":"46","year":"2002","unstructured":"Schulein, R., and C. Dehio. 2002. The VirB\/VirD4 type IV secretion system of Bartonella is essential for establishing intraerythrocytic infection. Mol. Microbiol. 46 : 1053-1067.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_54_2","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1073\/pnas.0406796102","volume":"102","year":"2005","unstructured":"Schulein, R., P. Guye, T. A. Rhomberg, M. C. Schmid, G. Schroder, A. C. Vergunst, I. Carena, and C. Dehio. 2005. A bipartite signal mediates the transfer of type IV secretion substrates of Bartonella henselae into human cells. Proc. Natl. Acad. Sci. USA 102 : 856-861.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_55_2","doi-asserted-by":"crossref","first-page":"1669","DOI":"10.1073\/pnas.95.4.1669","volume":"95","year":"1998","unstructured":"Segal, G., M. Purcell, and H. A. Shuman. 1998. Host cell killing and bacterial conjugation require overlapping sets of genes within a 22-kb region of the Legionella pneumophila genome. Proc. Natl. Acad. Sci. USA 95 : 1669-1674.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_56_2","doi-asserted-by":"crossref","first-page":"4866","DOI":"10.1073\/pnas.0501315102","volume":"102","year":"2005","unstructured":"Shohdy, N., J. A. Efe, S. D. Emr, and H. A. Shuman. 2005. Pathogen effector protein screening in yeast identifies Legionella factors that interfere with membrane trafficking. Proc. Natl. Acad. Sci. USA 102 : 4866-4871.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.17.4849-4855.2000"},{"key":"e_1_3_2_58_2","doi-asserted-by":"crossref","first-page":"7512","DOI":"10.1073\/pnas.93.15.7512","volume":"93","year":"1996","unstructured":"Spudich, G. M., D. Fernandez, X. R. Zhou, and P. J. Christie. 1996. Intermolecular disulfide bonds stabilize VirB7 homodimers and VirB7\/VirB9 heterodimers during biogenesis of the Agrobacterium tumefaciens T-complex transport apparatus. Proc. Natl. Acad. Sci. USA 93 : 7512-7517.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(90)85008-C"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.9.6048-6054.2005"},{"key":"e_1_3_2_61_2","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1099\/00221287-148-6-1637","volume":"148","year":"2002","unstructured":"Tauch, A., S. Schneiker, W. Selbitschka, A. Puhler, L. S. van Overbeek, K. Smalla, C. M. Thomas, M. J. Bailey, L. J. Forney, A. Weightman, P. Ceglowski, T. Pembroke, E. Tietze, G. Schroder, E. Lanka, and J. D. van Elsas. 2002. The complete nucleotide sequence and environmental distribution of the cryptic, conjugative, broad-host-range plasmid pIPO2 isolated from bacteria of the wheat rhizosphere. Microbiology 148 : 1637-1653.","journal-title":"Microbiology"},{"key":"e_1_3_2_62_2","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1126\/science.290.5493.979","volume":"290","year":"2000","unstructured":"Vergunst, A. C., B. Schrammeijer, A. den Dulk-Ras, C. M. de Vlaam, T. J. Regensburg-Tuink, and P. J. Hooykaas. 2000. VirB\/D4-dependent protein translocation from Agrobacterium into plant cells. Science 290 : 979-982.","journal-title":"Science"},{"key":"e_1_3_2_63_2","doi-asserted-by":"crossref","first-page":"5804","DOI":"10.1016\/S0021-9258(18)60637-4","volume":"263","year":"1988","unstructured":"Ward, J. E., D. E. Akiyoshi, D. Regier, A. Datta, M. P. Gordon, and E. W. Nester. 1988. Characterization of the virB operon from an Agrobacterium tumefaciens Ti plasmid. J. Biol. Chem. 263 : 5804-5814.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_64_2","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1099\/00221287-148-5-1439","volume":"148","year":"2002","unstructured":"Watarai, M., S. Makino, and T. Shirahata. 2002. An essential virulence protein of Brucella abortus, VirB4, requires an intact nucleoside-triphosphate-binding domain. Microbiology 148 : 1439-1446.","journal-title":"Microbiology"},{"key":"e_1_3_2_65_2","doi-asserted-by":"crossref","first-page":"2970","DOI":"10.1073\/pnas.90.7.2970","volume":"90","year":"1993","unstructured":"Weiss, A. A., F. D. Johnson, and D. L. Burns. 1993. Molecular characterization of an operon required for pertussis toxin secretion. Proc. Natl. Acad. Sci. USA 90 : 2970-2974.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_66_2","doi-asserted-by":"crossref","first-page":"3469","DOI":"10.1093\/nar\/17.9.3469","volume":"17","year":"1989","unstructured":"Woodcock, D. M., P. J. Crowther, J. Doherty, S. Jefferson, E. DeCruz, M. Noyer-Weidner, S. S. Smith, M. Z. Michael, and M. W. Graham. 1989. Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants. Nucleic Acids Res. 17 : 3469-3478.","journal-title":"Nucleic Acids Res."},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M502347200"},{"key":"e_1_3_2_68_2","doi-asserted-by":"crossref","first-page":"2849","DOI":"10.1016\/j.micinf.2006.09.002","volume":"8","year":"2006","unstructured":"Zygmunt, M. S., S. D. Hagius, J. V. Walker, and P. H. Elzer. 2006. Identification of Brucella melitensis 16M genes required for bacterial survival in the caprine host. Microbes Infect. 8 : 2849-2854.","journal-title":"Microbes Infect."}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.00406-08","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.00406-08","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T02:34:32Z","timestamp":1708914872000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.00406-08"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,7]]},"references-count":67,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2008,7]]}},"alternative-id":["10.1128\/JB.00406-08"],"URL":"https:\/\/doi.org\/10.1128\/jb.00406-08","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,7]]},"assertion":[{"value":"2008-03-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-04-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}