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However, their exact function in the formation of the complex cell wall structures of the<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Corynebacteriales<\/jats:named-content>, including the prominent pathogens<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Mycobacterium tuberculosis<\/jats:named-content>and<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Corynebacterium diphtheriae<\/jats:named-content>, remains unclear. Here, we analyzed the role of the LCP proteins LcpA and LcpB of<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Corynebacterium glutamicum<\/jats:named-content>, both of which localize at regions of nascent cell wall biosynthesis. A strain lacking<jats:italic>lcpB<\/jats:italic>did not show any growth-related or morphological phenotype under the tested conditions. In contrast, conditional silencing of the essential<jats:italic>lcpA<\/jats:italic>gene resulted in severe growth defects and drastic morphological changes. Compared to the wild-type cell wall, the cell wall of this mutant contained significantly less mycolic acids and a reduced amount of arabinogalactan. In particular, rhamnose, a specific sugar component of the linker that connects arabinogalactan and peptidoglycan, was decreased. Complementation studies of the<jats:italic>lcpA<\/jats:italic>-silencing strain with several mutated and truncated LcpA variants suggested that both periplasmic domains are essential for function whereas the cytoplasmic N-terminal part is dispensable. Successful complementation experiments with proteins of<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. tuberculosis<\/jats:named-content>and<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. diphtheriae<\/jats:named-content>revealed a conserved function of LCP proteins in these species. Finally, pyrophosphatase activity of LcpA was shown in an<jats:italic>in vitro<\/jats:italic>assay. Taken together, our results suggest that LCP proteins are responsible for the transfer of arabinogalactan onto peptidoglycan in actinobacterial species and support a crucial function of a so-far-uncharacterized C-terminal domain (LytR_C domain) which is frequently found at the C terminus of the LCP domain in this prokaryotic phylum.<\/jats:p><jats:p><jats:bold>IMPORTANCE<\/jats:bold>About one-third of the world's population is infected with<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Mycobacterium tuberculosis<\/jats:named-content>, and multiple-antibiotic resistance provokes the demand for novel antibiotics. The special cell wall architecture of<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Corynebacteriales<\/jats:named-content>is critical for treatments because it is either a direct target or a barrier that the drug has to cross. Here, we present the analysis of LcpA and LcpB of the closely related<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Corynebacterium glutamicum<\/jats:named-content>, the first of which is an essential protein involved in cell wall biogenesis. Our work provides a comprehensive characterization of the impact of LCP proteins on cell wall biogenesis in this medically and biotechnologically important class of bacteria. Special focus is set on the two periplasmic LcpA domains and their contributions to physiological function.<\/jats:p>","DOI":"10.1128\/jb.00406-16","type":"journal-article","created":{"date-parts":[[2016,8,23]],"date-time":"2016-08-23T02:18:58Z","timestamp":1471918738000},"page":"3045-3059","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":57,"title":["Impact of LytR-CpsA-Psr Proteins on Cell Wall Biosynthesis in Corynebacterium glutamicum"],"prefix":"10.1128","volume":"198","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9874-1151","authenticated-orcid":false,"given":"Meike","family":"Baumgart","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Bio- und Geowissenschaften, IBG-1: Biotechnologie, Forschungszentrum J\u00fclich, J\u00fclich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Schubert","sequence":"additional","affiliation":[{"name":"Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, Fakult\u00e4t Biologie, Planegg-Martinsried, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Bramkamp","sequence":"additional","affiliation":[{"name":"Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, Fakult\u00e4t Biologie, Planegg-Martinsried, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Frunzke","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Bio- und Geowissenschaften, IBG-1: Biotechnologie, Forschungszentrum J\u00fclich, J\u00fclich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-138-9-1949"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M005702200"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.7.2046-2051.1993"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku1243"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-617"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2011.358"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.567669"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00544-13"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12780"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2011.00276.x"},{"key":"e_1_3_3_12_2","first-page":"267","volume-title":"Corynebacteria: genomics and molecular biology","author":"Eggeling L","year":"2008","unstructured":"Eggeling L, Besra GS, Alderwick L. 2008. 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