{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T11:35:04Z","timestamp":1784806504045,"version":"3.55.0"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T00:00:00Z","timestamp":1738281600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T00:00:00Z","timestamp":1738281600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"HHS | National Institutes of Health","award":["GM118894"],"award-info":[{"award-number":["GM118894"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2025,1,31]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n              have a versatile metabolism; they can adapt to many stressors, including limited oxygen and nutrient availability. This versatility is especially important within a biofilm where multiple microenvironments are present. As a facultative anaerobe,\n              <jats:italic>P. aeruginosa<\/jats:italic>\n              can survive under anaerobic conditions utilizing denitrification. This process produces nitric oxide (NO) which has been shown to result in cell elongation. However, the molecular mechanism underlying this phenotype is poorly understood. Our laboratory has previously shown that NosP is a NO-sensitive hemoprotein that works with the histidine kinase NahK to regulate biofilm formation in\n              <jats:italic>P. aeruginosa<\/jats:italic>\n              . In this study, we identify NahK as a novel regulator of denitrification under anaerobic conditions. Under anaerobic conditions, deletion of\n              <jats:italic>nahK<\/jats:italic>\n              leads to a reduction of growth coupled with reduced transcriptional expression and activity of the denitrification reductases. Furthermore, during stationary phase under anaerobic conditions, \u0394\n              <jats:italic>nahK<\/jats:italic>\n              does not exhibit cell elongation, which is characteristic of\n              <jats:italic>P. aeruginosa<\/jats:italic>\n              . We determine the loss of cell elongation is due to changes in NO accumulation in \u0394\n              <jats:italic>nahK<\/jats:italic>\n              . We further provide evidence that NahK may regulate denitrification through modification of RsmA levels.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n              is an opportunistic multi-drug resistance pathogen that is associated with hospital-acquired infections.\n              <jats:italic>P. aeruginosa<\/jats:italic>\n              is highly virulent, in part due to its versatile metabolism and ability to form biofilms. Therefore, better understanding of the molecular mechanisms that regulate these processes should lead to new therapeutics to treat\n              <jats:italic>P. aeruginosa<\/jats:italic>\n              infections. The histidine kinase NahK has been previously shown to be involved in both nitric oxide (NO) signaling and quorum sensing through RsmA. The data presented here demonstrate that NahK is responsive to NO produced during denitrification to regulate cell morphology. Understanding the role of NahK in metabolism under anaerobic conditions has larger implications in determining its role in a heterogeneous metabolic environment such as a biofilm.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/jb.00408-24","type":"journal-article","created":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T19:17:35Z","timestamp":1733944655000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["The histidine kinase NahK regulates denitrification and nitric oxide accumulation through RsmA in\n            <i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.1128","volume":"207","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-9930-1092","authenticated-orcid":false,"given":"Danielle","family":"Guercio","sequence":"first","affiliation":[{"name":"Graduate Program in Molecular and Cellular Biology, Stony Brook University","place":["Stony Brook, 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