{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:32:26Z","timestamp":1791995546786,"version":"4.4.0"},"reference-count":59,"publisher":"American Society for Microbiology","issue":"23","license":[{"start":{"date-parts":[[2019,12,1]],"date-time":"2019-12-01T00:00:00Z","timestamp":1575158400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["Z1A AI000946"],"award-info":[{"award-number":["Z1A AI000946"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:p>\n            The intracellular pathogen\n            <jats:named-content content-type=\"genus-species\">Coxiella burnetii<\/jats:named-content>\n            employs a type 4B secretion system (T4BSS) that promotes growth by translocating effectors of eukaryotic pathways into host cells. T4BSS regulation modeled in\n            <jats:named-content content-type=\"genus-species\">Legionella pneumophila<\/jats:named-content>\n            indicates IcmS facilitates effector translocation. Here, we characterized type 4B secretion by a\n            <jats:italic>Coxiella<\/jats:italic>\n            \u0394\n            <jats:italic>icmS<\/jats:italic>\n            mutant that exhibits intracellular growth defects. T4BSS substrates demonstrated increased, equivalent, or decreased secretion by the \u0394\n            <jats:italic>icmS<\/jats:italic>\n            mutant relative to wild-type\n            <jats:italic>Coxiella<\/jats:italic>\n            . Similar to the\n            <jats:italic>Legionella<\/jats:italic>\n            T4BSS, IcmS dependency in\n            <jats:italic>Coxiella<\/jats:italic>\n            was determined by C-terminal and\/or internal secretion signals. However, IcmS inhibited secretion of some effectors by\n            <jats:italic>Coxiella<\/jats:italic>\n            that were previously shown to be translocated by\n            <jats:italic>Legionella<\/jats:italic>\n            . Thus,\n            <jats:italic>Coxiella<\/jats:italic>\n            has a unique IcmS regulatory mechanism that both positively and negatively regulates T4BSS export.\n          <\/jats:p>","DOI":"10.1128\/jb.00431-19","type":"journal-article","created":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T12:41:50Z","timestamp":1568032910000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Dependency of\n            <i>Coxiella burnetii<\/i>\n            Type 4B Secretion on the Chaperone IcmS"],"prefix":"10.1128","volume":"201","author":[{"given":"Charles L.","family":"Larson","sequence":"first","affiliation":[{"name":"Coxiella Pathogenesis Section, Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul A.","family":"Beare","sequence":"additional","affiliation":[{"name":"Coxiella Pathogenesis Section, Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6870-4619","authenticated-orcid":false,"given":"Robert A.","family":"Heinzen","sequence":"additional","affiliation":[{"name":"Coxiella Pathogenesis Section, Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.4.2469-2477.2005"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12096"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002056"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00175-11"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00881-12"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00071-13"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004013"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004286"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00758-17"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb-2016-0044"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1004380107"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01204-13"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12066"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10205"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01729-14"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1309195110"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006101"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01327-16"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1522811113"},{"key":"e_1_3_3_21_2","first-page":"431","volume-title":"Rickettsiae and rickettsial diseases","author":"Hackstadt T","year":"1981","unstructured":"Hackstadt T, Williams JC. 1981. Incorporation of macromolecular precursors by Coxiella burnetii in an axenic medium, p 431\u2013440. In Burgdorfer W, Anacker RL (ed), Rickettsiae and rickettsial diseases. 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