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While both\n              <jats:italic>Enterococcus faecalis<\/jats:italic>\n              and\n              <jats:italic>Lactobacillus crispatus<\/jats:italic>\n              are commensal residents of the vaginal tract, the molecular mechanisms that enable\n              <jats:italic>E. faecalis<\/jats:italic>\n              to take advantage of a vaginal biome with lower counts of lactobacilli to colonize the vaginal tract and induce aerobic vaginitis remain unknown. Here, we show that\n              <jats:italic>L. crispatus<\/jats:italic>\n              eradicates\n              <jats:italic>E. faecalis<\/jats:italic>\n              in a contact-independent manner. Using transposon sequencing to identify\n              <jats:italic>E. faecalis<\/jats:italic>\n              OG1RF transposon (Tn) mutants that are either under-represented or over-represented when co-cultured with\n              <jats:italic>L. crispatus<\/jats:italic>\n              , we found that Tn mutants with disruption in the\n              <jats:italic>dltABCD<\/jats:italic>\n              operon, that encodes the proteins responsible for the D-alanylation of teichoic acids, and\n              <jats:italic>OG1RF_11697<\/jats:italic>\n              encoding for an uncharacterized hypothetical protein are more susceptible to killing by\n              <jats:italic>L. crispatus<\/jats:italic>\n              . Inversely, Tn mutants with disruption in\n              <jats:italic>ldh1,<\/jats:italic>\n              which encodes for L-lactate dehydrogenase, are more resistant to\n              <jats:italic>L. crispatu<\/jats:italic>\n              s killing. Using the\n              <jats:italic>Galleria mellonella<\/jats:italic>\n              infection model, we show that co-injection of\n              <jats:italic>L. crispatus<\/jats:italic>\n              with\n              <jats:italic>E. faecalis<\/jats:italic>\n              OG1RF enhances larvae survival while this\n              <jats:italic>L. crispatus<\/jats:italic>\n              -mediated protection was lost in larvae co-infected with either\n              <jats:italic>L. crispatus<\/jats:italic>\n              and\n              <jats:italic>E. faecalis<\/jats:italic>\n              \u0394\n              <jats:italic>ldh1<\/jats:italic>\n              or \u0394\n              <jats:italic>ldh1<\/jats:italic>\n              \u0394\n              <jats:italic>ldh2<\/jats:italic>\n              strains. Last, using RNA sequencing to identify\n              <jats:italic>E. faecalis<\/jats:italic>\n              genes that are differently expressed in the presence of\n              <jats:italic>L. crispatus<\/jats:italic>\n              , we found major changes in the expression of genes associated with glycerophospholipid metabolism, central metabolism, and general stress responses. The findings in this study provide insights into how\n              <jats:italic>E. faecalis<\/jats:italic>\n              mitigate assaults by\n              <jats:italic>L. crispatus<\/jats:italic>\n              .\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                <jats:italic>Enterococcus faecalis<\/jats:italic>\n                is an opportunistic pathogen notorious for causing a multitude of infections. As vaginal commensals,\n                <jats:italic>E. faecalis<\/jats:italic>\n                must interact with\n                <jats:italic>Lactobacillus crispatus<\/jats:italic>\n                , but how\n                <jats:italic>E. faecalis<\/jats:italic>\n                overcomes or mitigate assaults by\n                <jats:italic>L. crispatus<\/jats:italic>\n                killing remains unknown. We show that\n                <jats:italic>L. crispatus<\/jats:italic>\n                eradicates\n                <jats:italic>E. faecalis<\/jats:italic>\n                temporally in a contact-independent manner. Using high-throughput molecular approaches, we identified genetic determinants that enable\n                <jats:italic>E. faecalis<\/jats:italic>\n                to compete with\n                <jats:italic>L. crispatus<\/jats:italic>\n                . This study represents an important first step for the identification of adaptive genetic traits required for enterococci to tolerate assaults by lactobacilli.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/jb.00438-24","type":"journal-article","created":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T14:04:20Z","timestamp":1738677860000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Genome-wide analysis of\n            <i>Enterococcus faecalis<\/i>\n            genes that facilitate interspecies competition with\n            <i>Lactobacillus crispatus<\/i>"],"prefix":"10.1128","volume":"207","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0568-8628","authenticated-orcid":true,"given":"Ling Ning","family":"Lam","sequence":"first","affiliation":[{"name":"Department of Oral Biology, College of Dentistry, University of Florida","place":["Gainesville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn E.","family":"Savage","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, College of Dentistry, University of Florida","place":["Gainesville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille N.","family":"Shakir","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, College of Dentistry, University of Florida","place":["Gainesville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0791-5863","authenticated-orcid":true,"given":"Jos\u00e9 A.","family":"Lemos","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, College of Dentistry, University of Florida","place":["Gainesville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3432"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI43918"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00537-20"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofad238"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.7257"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics12040704"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinpr.2023.100336"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.2147\/RRU.S273663"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11020238"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcin.2014.09.013"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2761"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00008-19"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0107-z"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241411577"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics12020328"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11061614"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/femspd\/ftac010"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.5851\/kosfa.2014.34.5.614"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2013.01.005"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/834151"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1620432114"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2016.09.004"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02638-17"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00378-17"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11051210"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01182-17"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.00615-21"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05438-x"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41396-022-01248-1"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/femspd\/ftx009"},{"key":"e_1_3_5_32_2","first-page":"21","article-title":"Antimicrobial activity of different Lactobacillus species against multi- drug resistant clinical isolates of Pseudomonas aeruginosa","volume":"3","author":"Jamalifar H","year":"2011","unstructured":"Jamalifar H, Rahimi H, Samadi N, Shahverdi A, Sharifian Z, Hosseini F, Eslahi H, Fazeli M. 2011. 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