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Two key enzymes in purine salvage pathways are IMP dehydrogenase (GuaB) and GMP synthase (GuaA), encoded by the<jats:italic>guaB<\/jats:italic>and<jats:italic>guaA<\/jats:italic>genes, respectively. While these genes are typically found on the chromosome in most bacterial pathogens, the<jats:italic>guaAB<\/jats:italic>operon of<jats:italic>B<\/jats:italic><jats:italic>orrelia burgdorferi<\/jats:italic>is present on plasmid cp26, which also harbors a number of genes critical for<jats:italic>B. burgdorferi<\/jats:italic>viability. Using molecular genetics and an experimental model of the tick-mouse infection cycle, we demonstrate that the enzymatic activities encoded by the<jats:italic>guaAB<\/jats:italic>operon are essential for<jats:italic>B. burgdorferi<\/jats:italic>mouse infectivity and provide a growth advantage to spirochetes in the tick. These data indicate that the GuaA and GuaB proteins are critical for the survival of<jats:italic>B. burgdorferi<\/jats:italic>in the infection cycle and highlight a potential difference in the requirements for purine salvage in the disparate mammalian and tick environments.<\/jats:p>","DOI":"10.1128\/jb.00450-09","type":"journal-article","created":{"date-parts":[[2009,8,8]],"date-time":"2009-08-08T00:54:47Z","timestamp":1249692887000},"page":"6231-6241","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":83,"title":["GuaA and GuaB Are Essential for<i>B<\/i><i>orrelia burgdorferi<\/i>Survival in the Tick-Mouse Infection Cycle"],"prefix":"10.1128","volume":"191","author":[{"given":"Mollie W.","family":"Jewett","sequence":"first","affiliation":[{"name":"Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin A.","family":"Lawrence","sequence":"additional","affiliation":[{"name":"Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron","family":"Bestor","sequence":"additional","affiliation":[{"name":"Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca","family":"Byram","sequence":"additional","affiliation":[{"name":"Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840"},{"name":"Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82701"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Gherardini","sequence":"additional","affiliation":[{"name":"Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia A.","family":"Rosa","sequence":"additional","affiliation":[{"name":"Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"521","volume":"57","year":"1984","unstructured":"Barbour, A. 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