{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T13:47:46Z","timestamp":1787060866229,"version":"build-2736575974"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"16","license":[{"start":{"date-parts":[[2008,8,15]],"date-time":"2008-08-15T00:00:00Z","timestamp":1218758400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2008,8,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Secondary transporters of citrate in complex with metal ions belong to the bacterial CitMHS family, about which little is known. The transport of metal-citrate complexes in\n                    <jats:italic>Streptomyces coelicolor<\/jats:italic>\n                    has been investigated. The best cofactor for citrate uptake in\n                    <jats:italic>Streptomyces coelicolor<\/jats:italic>\n                    is Fe\n                    <jats:sup>3+<\/jats:sup>\n                    , but uptake was also noted for Ca\n                    <jats:sup>2+<\/jats:sup>\n                    , Pb\n                    <jats:sup>2+<\/jats:sup>\n                    , Ba\n                    <jats:sup>2+<\/jats:sup>\n                    , and Mn\n                    <jats:sup>2+<\/jats:sup>\n                    . Uptake was not observed with the Mg\n                    <jats:sup>2+<\/jats:sup>\n                    , Ni\n                    <jats:sup>2+<\/jats:sup>\n                    , or Co\n                    <jats:sup>2+<\/jats:sup>\n                    cofactor. The transportation of iron- and calcium-citrate makes these systems unique among the CitMHS family members reported to date. No complementary uptake akin to that observed for the CitH (Ca\n                    <jats:sup>2+<\/jats:sup>\n                    , Ba\n                    <jats:sup>2+<\/jats:sup>\n                    , Sr\n                    <jats:sup>2+<\/jats:sup>\n                    ) and CitM (Mg\n                    <jats:sup>2+<\/jats:sup>\n                    , Ni\n                    <jats:sup>2+<\/jats:sup>\n                    , Mn\n                    <jats:sup>2+<\/jats:sup>\n                    , Co\n                    <jats:sup>2+<\/jats:sup>\n                    , Zn\n                    <jats:sup>2+<\/jats:sup>\n                    ) systems of\n                    <jats:italic>Bacillus subtilis<\/jats:italic>\n                    was noted. Competitive experiments using EGTA confirmed that metal-citrate complex formation promoted citrate uptake. Uptake of free citrate was not observed. The open reading frame postulated as being responsible for the metal-citrate transport observed in\n                    <jats:italic>Streptomyces coelicolor<\/jats:italic>\n                    was cloned and overexpressed in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    strains with the primary Fe\n                    <jats:sup>3+<\/jats:sup>\n                    -citrate transport system (\n                    <jats:italic>fecABCDE<\/jats:italic>\n                    ) removed. Functional expression was successful, with uptake of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -citrate, Fe\n                    <jats:sup>3+<\/jats:sup>\n                    -citrate, and Pb\n                    <jats:sup>2+<\/jats:sup>\n                    -citrate observed. No free-citrate transport was observed in IPTG (isopropyl-\u03b2-\n                    <jats:sc>d<\/jats:sc>\n                    -thiogalactopyranoside)-induced or -uninduced\n                    <jats:italic>E. coli<\/jats:italic>\n                    . Metabolism of the Fe\n                    <jats:sup>3+<\/jats:sup>\n                    -citrate and Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -citrate complexes, but not the Pb\n                    <jats:sup>2+<\/jats:sup>\n                    -citrate complex, was observed. Rationalization is based on the difference in metal-complex coordination upon binding of the metal by citrate.\n                  <\/jats:p>","DOI":"10.1128\/jb.00456-08","type":"journal-article","created":{"date-parts":[[2008,6,13]],"date-time":"2008-06-13T22:15:51Z","timestamp":1213395351000},"page":"5616-5623","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Functional Characterization and Metal Ion Specificity of the Metal-Citrate Complex Transporter from\n                    <i>Streptomyces coelicolor<\/i>"],"prefix":"10.1128","volume":"190","author":[{"given":"Joshua J.","family":"Lensbouer","sequence":"first","affiliation":[{"name":"Department of Chemistry, Center for Science and Technology, Syracuse University, Syracuse, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ami","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Center for Science and Technology, Syracuse University, Syracuse, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph P.","family":"Sirianni","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Center for Science and Technology, Syracuse University, Syracuse, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert P.","family":"Doyle","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Center for Science and Technology, Syracuse University, Syracuse, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"18140","DOI":"10.1074\/jbc.272.29.18140","volume":"272","year":"1997","unstructured":"Bandell, M., V. 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