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The study of\n            <jats:italic>S. aureus<\/jats:italic>\n            mutants with resistance to lysostaphin-mediated killing has revealed biosynthetic pathways for cell wall assembly. To identify additional genes involved in cell wall envelope biosynthesis, we have screened a collection of\n            <jats:italic>S. aureus<\/jats:italic>\n            strain Newman transposon mutants for lysostaphin resistance.\n            <jats:italic>Bursa aurealis<\/jats:italic>\n            insertion in SAV2335, encoding a polytopic membrane protein with predicted protease domain, caused a high degree of lysostaphin resistance, similar to the case for a previously described\n            <jats:italic>femAB<\/jats:italic>\n            promoter mutant. In contrast to the case for this\n            <jats:italic>femAB<\/jats:italic>\n            mutant, transposon insertion in SAV2335, herein named\n            <jats:italic>lyrA<\/jats:italic>\n            (\n            <jats:italic>ly<\/jats:italic>\n            sostaphin\n            <jats:italic>r<\/jats:italic>\n            esistance\n            <jats:italic>A<\/jats:italic>\n            ), did not cause gross alterations of cell wall cross bridges such as truncations of pentaglycine to tri- or monoglycine. Also, inactivation of LyrA in a methicillin-resistant\n            <jats:italic>S. aureus<\/jats:italic>\n            strain did not precipitate a decrease in \u03b2-lactam resistance as observed for\n            <jats:italic>fem<\/jats:italic>\n            (\n            <jats:italic>f<\/jats:italic>\n            actor\n            <jats:italic>e<\/jats:italic>\n            ssential for\n            <jats:italic>m<\/jats:italic>\n            ethicillin resistance) mutants. Lysostaphin bound to the cell wall envelopes of\n            <jats:italic>lyrA<\/jats:italic>\n            mutants in a manner similar to that for wild-type staphylococci. Lysostaphin resistance of\n            <jats:italic>lyrA<\/jats:italic>\n            mutants is attributable to altered cell wall envelope properties and may in part be due to increased abundance of altered cross bridges. Other\n            <jats:italic>lyr<\/jats:italic>\n            mutants with intermediate lysostaphin resistance carried\n            <jats:italic>bursa aurealis<\/jats:italic>\n            insertions in genes specifying GTP pyrophosphokinase or enzymes of the purine biosynthetic pathway.\n          <\/jats:p>","DOI":"10.1128\/jb.00457-06","type":"journal-article","created":{"date-parts":[[2006,8,21]],"date-time":"2006-08-21T17:14:16Z","timestamp":1156180456000},"page":"6286-6297","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":104,"title":["<i>Staphylococcus aureus<\/i>\n            Mutants with Increased Lysostaphin Resistance"],"prefix":"10.1128","volume":"188","author":[{"given":"Angelika","family":"Gru\u0308ndling","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Chicago, Chicago, Illinois 60637"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique M.","family":"Missiakas","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Chicago, Chicago, Illinois 60637"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olaf","family":"Schneewind","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Chicago, Chicago, Illinois 60637"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200101043440110"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"4789","DOI":"10.1002\/j.1460-2075.1996.tb00859.x","volume":"15","year":"1996","unstructured":"Baba, T., and O. 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