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With the exception of EsxB,<jats:italic>B. anthracis<\/jats:italic>proteins harbor C-terminal extensions that are appended to canonical WXG domains. When cultured in liquid broth,<jats:italic>B. anthracis<\/jats:italic>secretes two substrates, EsxB and EsxW, into the extracellular environment. EsxB is required for the stability and secretion of EsxW; however, EsxW is dispensable for EsxB secretion. In agreement with the hypothesis that EsxB binding to substrates promotes recognition and secretion by the type VII pathway, EsxB is reported to interact with EsxB and EsxW. Unlike deletions in mycobacterial EsxB, deletion of five N- or C-terminal residues does not affect the ability of mutant<jats:italic>B. anthracis<\/jats:italic>EsxB to travel the type VII pathway and initiate secretion of EsxW. Translational fusion of ubiquitin to the N or C terminus of EsxB also had no effect, while ubiquitin insertion into the center turn abrogated secretion. Anthrax-infected guinea pigs mounted humoral immune responses to EsxB, EsxP, and EsxW, which suggests that<jats:italic>B. anthracis<\/jats:italic>activates the type VII secretion pathway during infection.<\/jats:p>","DOI":"10.1128\/jb.00458-08","type":"journal-article","created":{"date-parts":[[2008,8,23]],"date-time":"2008-08-23T00:45:50Z","timestamp":1219452350000},"page":"7004-7011","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":80,"title":["ESAT-6-Like Protein Secretion in<i>Bacillus anthracis<\/i>"],"prefix":"10.1128","volume":"190","author":[{"given":"Gabriella","family":"Garufi","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, Illinois 60637"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Butler","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, Illinois 60637"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Missiakas","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, Illinois 60637"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1038\/nrmicro1773","volume":"5","year":"2007","unstructured":"Abdallah, A. 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