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The ability of\n            <jats:italic>L. pneumophila<\/jats:italic>\n            to replicate within host cells requires the establishment of a specialized compartment that evades normal phagolysosome fusion called the\n            <jats:italic>Legionella<\/jats:italic>\n            -containing vacuole (LCV). Elucidation of the biochemical composition of the LCV and the identification of the regulatory signals sensed during intracellular replication are inherently challenging.\n            <jats:sc>l<\/jats:sc>\n            -Arginine is a critical nutrient in the metabolism of both prokaryotic and eukaryotic organisms. We showed that the\n            <jats:italic>L. pneumophila<\/jats:italic>\n            arginine repressor homolog, ArgR, is required for maximal intracellular growth in the unicellular host\n            <jats:italic>Acanthamoeba castellanii<\/jats:italic>\n            . In this study, we present evidence that the concentration of\n            <jats:sc>l<\/jats:sc>\n            -arginine in the LCV is sensed by ArgR to produce an intracellular transcriptional response. We characterized the\n            <jats:italic>L. pneumophila<\/jats:italic>\n            ArgR regulon by global gene expression analysis, identified genes highly affected by ArgR, showed that ArgR repression is dependent upon the presence of\n            <jats:sc>l<\/jats:sc>\n            -arginine, and demonstrated that ArgR-regulated genes are derepressed during intracellular growth. Additional targets of ArgR that may account for the\n            <jats:italic>argR<\/jats:italic>\n            mutant's intracellular multiplication defect are discussed. These results suggest that\n            <jats:sc>l<\/jats:sc>\n            -arginine availability functions as a regulatory signal during\n            <jats:italic>Legionella<\/jats:italic>\n            intracellular growth.\n          <\/jats:p>","DOI":"10.1128\/jb.00465-10","type":"journal-article","created":{"date-parts":[[2010,7,12]],"date-time":"2010-07-12T01:28:36Z","timestamp":1278898116000},"page":"4504-4516","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":38,"title":["ArgR-Regulated Genes Are Derepressed in the\n            <i>Legionella<\/i>\n            -Containing Vacuole"],"prefix":"10.1128","volume":"192","author":[{"given":"Galadriel","family":"Hovel-Miner","sequence":"first","affiliation":[{"name":"Department of Microbiology &amp; Immunology, Columbia University Medical Center, 701 West 168th Street, New York, New York 10032"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastien P.","family":"Faucher","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, Columbia University Medical Center, 701 West 168th Street, New York, New York 10032"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Charpentier","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, Columbia University Medical Center, 701 West 168th Street, New York, New York 10032"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Howard A.","family":"Shuman","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, Columbia University Medical Center, 701 West 168th Street, New York, New York 10032"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.6.2022-2028.1996"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01081-08"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.4.1351-1363.2006"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01493-07"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1189\/jlb.0206079","volume":"79","year":"2006","unstructured":"Appelberg, R. 2006. 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