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The targets of<jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>-produced H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>have not been discovered, in part because of a lack of knowledge about the underlying molecular mechanism. We demonstrated that an increased density of<jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>kills<jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>by means of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>produced by two enzymes, SpxB and LctO. We discovered that SpxB\/LctO-produced H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>is converted into a hydroxyl radical (<jats:sup>\u00b7<\/jats:sup>OH) that rapidly intoxicates and kills<jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>. We successfully inhibited the toxicity of<jats:sup>\u00b7<\/jats:sup>OH with three different scavengers and detected<jats:sup>\u00b7<\/jats:sup>OH in the supernatant. 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