{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:29:44Z","timestamp":1787016584507,"version":"build-2736575974"},"reference-count":71,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["CEX2020-00108-M"],"award-info":[{"award-number":["CEX2020-00108-M"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["PGC2018-097151-B-I00"],"award-info":[{"award-number":["PGC2018-097151-B-I00"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["PID2021-126121-NB-I00"],"award-info":[{"award-number":["PID2021-126121-NB-I00"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"name":"German Research Council","award":["TH831 10\/1"],"award-info":[{"award-number":["TH831 10\/1"]}]},{"DOI":"10.13039\/501100003043","name":"EMBO","doi-asserted-by":"publisher","award":["Scientific Exchange Grant 9458"],"award-info":[{"award-number":["Scientific Exchange Grant 9458"]}],"id":[{"id":"10.13039\/501100003043","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2026,2,19]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Homologs of the polar landmark proteins HubP and FimV are widespread among bacterial species. They all share several common features, including a periplasmic LysM-like domain, a transmembrane region, an extensive cytoplasmic domain enriched in acidic amino acids, and a C-terminal tetrapeptid-repeat (TPR) domain referred to as the FimV domain. Apart from these conserved general features, however, the proteins exhibit little homology across different bacterial genera. Here, we characterized\n                      <jats:italic toggle=\"yes\">Pseudomonas putida<\/jats:italic>\n                      FimV (\n                      <jats:italic toggle=\"yes\">Pp<\/jats:italic>\n                      FimV) with respect to cellular processes involving FimV or HubP in other species. We found that\n                      <jats:italic toggle=\"yes\">Pp<\/jats:italic>\n                      FimV nonspecifically binds to peptidoglycan via its periplasmic LysM domain, which, together with an immunoglobulin-like domain, is necessary for proper polar positioning.\n                      <jats:italic toggle=\"yes\">Pp<\/jats:italic>\n                      FimV is required for normal flagellar-mediated swimming and the placement of the chemotaxis system. However,\n                      <jats:italic toggle=\"yes\">Pp<\/jats:italic>\n                      FimV is not involved in regulating the number of flagellar filaments, chromosome segregation, or type IV pilus-dependent surface motility. Thus,\n                      <jats:italic toggle=\"yes\">Pp<\/jats:italic>\n                      FimV has surprisingly little functional overlap with, for example, HubP from\n                      <jats:italic toggle=\"yes\">Vibrio<\/jats:italic>\n                      sp. or\n                      <jats:italic toggle=\"yes\">Shewanella putrefaciens<\/jats:italic>\n                      or with FimV from\n                      <jats:italic toggle=\"yes\">P. aeruginosa. Pp<\/jats:italic>\n                      FimV was unable to compensate for the loss of\n                      <jats:italic toggle=\"yes\">Sp<\/jats:italic>\n                      HubP with regard to swimming in soft agar, and\n                      <jats:italic toggle=\"yes\">vice versa<\/jats:italic>\n                      . Domain swapping between\n                      <jats:italic toggle=\"yes\">Sp<\/jats:italic>\n                      HubP and\n                      <jats:italic toggle=\"yes\">Pp<\/jats:italic>\n                      FimV revealed that differences in the cytoplasmic region between the transmembrane region and the C-terminal FimV domain likely account for the proteins\u2019 distinct functions in flagella-mediated swimming. This suggests that FimV and HubP are structural homologs that have evolved to perform different, species-specific functions.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Many bacterial species possess landmark proteins that organize the bacterial cell and localize specific cellular processes to the cell\u2019s polar regions by directing client proteins or protein complexes to their designated positions. FimV and its homolog HubP are landmark proteins found in many species of the gammaproteobacteria, but their roles are not well understood. Here, we demonstrate that only certain functions related to flagella-mediated motility appear to be conserved between\n                        <jats:italic toggle=\"yes\">Pseudomonas putida<\/jats:italic>\n                        FimV and\n                        <jats:italic toggle=\"yes\">Shewanella putrefaciens<\/jats:italic>\n                        HubP. This finding suggests a significant degree of functional diversity.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/jb.00497-25","type":"journal-article","created":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T14:57:20Z","timestamp":1768229840000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Functional characterization of the polar organizer protein FimV in\n                    <i>Pseudomonas putida<\/i>"],"prefix":"10.1128","volume":"208","author":[{"given":"Lisa Marie","family":"Schmidt","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/033eqas34","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Microbiology and Molecular Biology, Justus-Liebig-Universit\u00e4t Gie\u00dfen","place":["Gie\u00dfen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4234-4325","authenticated-orcid":true,"given":"Marta","family":"Pulido-S\u00e1nchez","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01v5e3436","id-type":"ROR","asserted-by":"publisher"}],"name":"Centro Andaluz de Biologia del Desarrollo, Universidad Pablo de Olavide\/Consejo Superior de Investigaciones Cientificas\/Junta de Andalucia","place":["Seville, Spain"]},{"id":[{"id":"https:\/\/ror.org\/02z749649","id-type":"ROR","asserted-by":"publisher"}],"name":"Departamento de Biologia Molecular e Ingenieria Bioquimica, Universidad Pablo de Olavide","place":["Seville, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6435-5642","authenticated-orcid":true,"given":"Anke","family":"Treuner-Lange","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05r7n9c40","id-type":"ROR","asserted-by":"publisher"}],"name":"Max Planck Institute for Terrestrial Microbiology","place":["Marburg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lukas","family":"Zehner","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/033eqas34","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Microbiology and Molecular Biology, Justus-Liebig-Universit\u00e4t Gie\u00dfen","place":["Gie\u00dfen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9022-3811","authenticated-orcid":true,"given":"Aroa","family":"L\u00f3pez-S\u00e1nchez","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01v5e3436","id-type":"ROR","asserted-by":"publisher"}],"name":"Centro Andaluz de Biologia del Desarrollo, Universidad Pablo de Olavide\/Consejo Superior de Investigaciones Cientificas\/Junta de Andalucia","place":["Seville, Spain"]},{"id":[{"id":"https:\/\/ror.org\/02z749649","id-type":"ROR","asserted-by":"publisher"}],"name":"Departamento de Biologia Molecular e Ingenieria Bioquimica, Universidad Pablo de Olavide","place":["Seville, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5995-718X","authenticated-orcid":true,"given":"Felipe","family":"Cava","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05kb8h459","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Biology, The Laboratory for Molecular Infection Medicine Sweden (MIMS), Ume\u00e5 Center for Microbial Research (UCMR), Science for Life Laboratory (SciLifeLab), Ume\u00e5 University","place":["Ume\u00e5, Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4866-6798","authenticated-orcid":true,"given":"Fernando","family":"Govantes","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01v5e3436","id-type":"ROR","asserted-by":"publisher"}],"name":"Centro Andaluz de Biologia del Desarrollo, Universidad Pablo de Olavide\/Consejo Superior de Investigaciones Cientificas\/Junta de Andalucia","place":["Seville, Spain"]},{"id":[{"id":"https:\/\/ror.org\/02z749649","id-type":"ROR","asserted-by":"publisher"}],"name":"Departamento de Biologia Molecular e Ingenieria Bioquimica, Universidad Pablo de Olavide","place":["Seville, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7292-4884","authenticated-orcid":true,"given":"Kai M.","family":"Thormann","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/033eqas34","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Microbiology and Molecular Biology, Justus-Liebig-Universit\u00e4t Gie\u00dfen","place":["Gie\u00dfen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.138628"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201403136"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.199869.112"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13152"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41522-022-00297-w"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00534-18"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-146-6-1321"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/genes13050877"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1315722111"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.31058"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00793-17"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14070"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-43051-7"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1009991"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14268"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00322-16"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13410"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02103-16"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00188-17"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14236"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008149"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.655239"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2024.128033"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-59536-9"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2018.11.008"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.93004"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03819-2"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2025.168967"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.12.7458"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-16428-9"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5269"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.14.4068-4076.2000"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12323"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)81481-3"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.01402"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008703"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2019.183030"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-032421-110850"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.15857"},{"key":"e_1_3_3_41_2","doi-asserted-by":"crossref","unstructured":"Pulido-S\u00e1nchez M L\u00f3pez-S\u00e1nchez A Govantes F. 2025. FimV ParC and ParP coordinate polar location of the chemosensory arrays in Pseudomonas putida","DOI":"10.1101\/2025.10.27.684771"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.3.642-650.1994"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-2920.2002.00366.x"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2007.01286.x"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-019-0195-4"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuab053"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26822-0"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01048-10"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-50274-4"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/14.24.10001"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06211.x"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00294-014-0471-9"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2018.00923"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00294-016-0630-2"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1508536112"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1994.1582"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-33564-7"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1322889111"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1003492"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12499"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07135.x"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-010-0590-8"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.22.6579-6589.2001"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00512-10"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1318"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.06.001"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth765"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-3676-2_2"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)49903-1"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-021-01156-3"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","unstructured":"Hallgren J Tsirigos KD Pedersen MD Almagro Armenteros JJ Marcatili P Nielsen H Krogh A Winther O. 2022. DeepTMHMM predicts alpha and beta transmembrane proteins using deep neural networks. bioRxiv. doi:10.1101\/2022.04.08.487609","DOI":"10.1101\/2022.04.08.487609"},{"key":"e_1_3_3_72_2","doi-asserted-by":"publisher","DOI":"10.1385\/1-59259-890-0:571"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.00497-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.00497-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T14:01:27Z","timestamp":1772805687000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.00497-25"}},"subtitle":[],"editor":[{"given":"George","family":"O'Toole","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,2,19]]},"references-count":71,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2,19]]}},"alternative-id":["10.1128\/jb.00497-25"],"URL":"https:\/\/doi.org\/10.1128\/jb.00497-25","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,19]]},"assertion":[{"value":"2025-10-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00497-25"}}