{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T18:27:31Z","timestamp":1784226451403,"version":"3.55.0"},"reference-count":84,"publisher":"American Society for Microbiology","issue":"22","license":[{"start":{"date-parts":[[2019,11,15]],"date-time":"2019-11-15T00:00:00Z","timestamp":1573776000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2019,11,15]]},"abstract":"<jats:p>\n            Regulation of metabolism and virulence in\n            <jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>\n            is not well understood. Here, we show that\n            <jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>\n            small RNA 12 (CbsR12) is highly transcribed in the metabolically active large-cell variant compared to the nonreplicative small-cell variant. We show that CbsR12 directly regulates several genes involved in metabolism, along with a type IV effector gene, in\n            <jats:italic>trans<\/jats:italic>\n            . In addition, we demonstrate that CbsR12 binds to CsrA-2\n            <jats:italic>in vitro<\/jats:italic>\n            and induces autoaggregation and biofilm formation when transcribed ectopically in\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            , consistent with other CsrA-sequestering sRNAs. These results implicate CbsR12 in the indirect regulation of a number of genes via CsrA-mediated regulatory activities. The results also support CbsR12 as a crucial regulatory component early on in a mammalian cell infection.\n          <\/jats:p>","DOI":"10.1128\/jb.00524-19","type":"journal-article","created":{"date-parts":[[2019,8,21]],"date-time":"2019-08-21T10:27:15Z","timestamp":1566383235000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["A CsrA-Binding,\n            <i>trans<\/i>\n            -Acting sRNA of\n            <i>Coxiella burnetii<\/i>\n            Is Necessary for Optimal Intracellular Growth and Vacuole Formation during Early Infection of Host Cells"],"prefix":"10.1128","volume":"201","author":[{"given":"Shaun","family":"Wachter","sequence":"first","affiliation":[{"name":"Program in Cellular, Molecular, and Microbial Biology, Division of Biological Sciences, University of Montana, Missoula, Montana, 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