{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T19:23:18Z","timestamp":1762543398950,"version":"3.40.5"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2021,3,8]],"date-time":"2021-03-08T00:00:00Z","timestamp":1615161600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2021,3,8]]},"abstract":"<jats:p>The entire 7-gene cluster for the diglycosylated bacteriocin glycocin F (GccF), including the natural promoters responsible for<jats:italic>gcc<\/jats:italic>gene expression, has been ligated into the<jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>-lactic acid bacteria (LAB) shuttle vector pRV610 to produce the easily modifiable 11.2-kbp plasmid pRV610<jats:italic>gcc<\/jats:italic>for the efficient production of glycocin F analogues. In contrast to the refactoring approach, chemical synthesis, or chemoenzymatic synthesis, all of which have been successfully used to probe glycocin structure and function, this plasmid can also be used to probe<jats:italic>in vivo<\/jats:italic>the evolutionary constraints on glycocin scaffolds and their processing by the maturation pathway machinery, thus increasing understanding of the enzymes involved, the order in which they act, and how they are regulated.<\/jats:p>","DOI":"10.1128\/jb.00529-20","type":"journal-article","created":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T13:29:30Z","timestamp":1610717370000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Optimized Genetic Tools Allow the Biosynthesis of Glycocin F and Analogues Designed To Test the Roles of<i>gcc<\/i>Cluster Genes in Bacteriocin Production"],"prefix":"10.1128","volume":"203","author":[{"given":"Brittany J.","family":"Drummond","sequence":"first","affiliation":[{"name":"School of Fundamental Sciences, Massey University, Palmerston North, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trevor S.","family":"Loo","sequence":"additional","affiliation":[{"name":"School of Fundamental Sciences, Massey University, Palmerston North, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1928-0431","authenticated-orcid":true,"given":"Mark L.","family":"Patchett","sequence":"additional","affiliation":[{"name":"School of Fundamental Sciences, Massey University, Palmerston North, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gillian E.","family":"Norris","sequence":"additional","affiliation":[{"name":"School of Fundamental Sciences, Massey University, Palmerston North, New Zealand"},{"name":"Maurice Wilkins Centre for Molecular Biodiscovery, The University of Auckland, Auckland, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"doi-asserted-by":"publisher","key":"e_1_3_3_2_2","DOI":"10.1038\/nrmicro1273"},{"doi-asserted-by":"publisher","key":"e_1_3_3_3_2","DOI":"10.1079\/BJN2002631"},{"doi-asserted-by":"publisher","key":"e_1_3_3_4_2","DOI":"10.1038\/ajg.2011.304"},{"doi-asserted-by":"publisher","key":"e_1_3_3_5_2","DOI":"10.1038\/nrmicro2259"},{"doi-asserted-by":"publisher","key":"e_1_3_3_6_2","DOI":"10.1007\/s00253-016-7343-9"},{"doi-asserted-by":"publisher","key":"e_1_3_3_7_2","DOI":"10.1016\/j.sbi.2016.09.003"},{"doi-asserted-by":"publisher","key":"e_1_3_3_8_2","DOI":"10.1039\/c2np20085f"},{"doi-asserted-by":"publisher","key":"e_1_3_3_9_2","DOI":"10.1038\/nchembio.509"},{"doi-asserted-by":"publisher","key":"e_1_3_3_10_2","DOI":"10.1021\/cb4008106"},{"doi-asserted-by":"publisher","key":"e_1_3_3_11_2","DOI":"10.1016\/j.febslet.2011.01.023"},{"doi-asserted-by":"publisher","key":"e_1_3_3_12_2","DOI":"10.1021\/bi200217u"},{"doi-asserted-by":"publisher","key":"e_1_3_3_13_2","DOI":"10.1002\/1873-3468.13708"},{"doi-asserted-by":"publisher","key":"e_1_3_3_14_2","DOI":"10.1016\/j.ijfoodmicro.2009.10.021"},{"doi-asserted-by":"publisher","key":"e_1_3_3_15_2","DOI":"10.1128\/AEM.02772-08"},{"doi-asserted-by":"publisher","key":"e_1_3_3_16_2","DOI":"10.1128\/AEM.00940-15"},{"doi-asserted-by":"publisher","key":"e_1_3_3_17_2","DOI":"10.1021\/ja411159k"},{"doi-asserted-by":"publisher","key":"e_1_3_3_18_2","DOI":"10.1038\/s41467-019-09065-5"},{"doi-asserted-by":"publisher","key":"e_1_3_3_19_2","DOI":"10.1021\/acschembio.8b00599"},{"key":"e_1_3_3_20_2","volume-title":"The bacteriostatic spectrum and inhibitory mechansim of glycocin F, a bacteriocin from Lactobacillus plantarum KW30","author":"Kerr A","year":"2013","unstructured":"Kerr A. 2013. MSc thesis. The bacteriostatic spectrum and inhibitory mechansim of glycocin F, a bacteriocin from Lactobacillus plantarum KW30. Massey University, Palmerston North, New Zealand."},{"doi-asserted-by":"publisher","key":"e_1_3_3_21_2","DOI":"10.1021\/acschembio.8b00055"},{"doi-asserted-by":"publisher","key":"e_1_3_3_22_2","DOI":"10.1039\/c7sc04383j"},{"key":"e_1_3_3_23_2","volume-title":"The role of the N-acetylglucosamine phosphoenol-pyruvate phosphotransferase system from Lactobacillus plantarum 8014 in the mechanism of action of glycocin F","author":"Bailie M","year":"2017","unstructured":"Bailie M. 2017. MSc thesis. The role of the N-acetylglucosamine phosphoenol-pyruvate phosphotransferase system from Lactobacillus plantarum 8014 in the mechanism of action of glycocin F. Massey University, Palmerston North, New Zealand."},{"doi-asserted-by":"publisher","key":"e_1_3_3_24_2","DOI":"10.1093\/femsle\/fny261"},{"doi-asserted-by":"publisher","key":"e_1_3_3_25_2","DOI":"10.1021\/ja2075168"},{"doi-asserted-by":"publisher","key":"e_1_3_3_26_2","DOI":"10.1016\/j.peptides.2009.05.014"},{"doi-asserted-by":"publisher","key":"e_1_3_3_27_2","DOI":"10.1128\/JB.00769-15"},{"doi-asserted-by":"publisher","key":"e_1_3_3_28_2","DOI":"10.1074\/jbc.M201158200"},{"doi-asserted-by":"publisher","key":"e_1_3_3_29_2","DOI":"10.1016\/j.str.2008.04.003"},{"key":"e_1_3_3_30_2","volume-title":"Metagenomics and its applications in agriculture, biomedicine, and environmental studies","author":"Li RW","year":"2011","unstructured":"Li RW. 2011. Metagenomics and its applications in agriculture, biomedicine, and environmental studies. Nova Science Publishers, Inc., Hauppauge, N.Y."},{"doi-asserted-by":"publisher","key":"e_1_3_3_31_2","DOI":"10.1016\/j.plasmid.2008.08.002"},{"key":"e_1_3_3_32_2","first-page":"44","volume-title":"The bacteriostatic diglycocylated bacteriocin glycocin F targets a sugar-specific transporter","author":"Drower K","year":"2014","unstructured":"Drower K. 2014. MSc thesis. The bacteriostatic diglycocylated bacteriocin glycocin F targets a sugar-specific transporter, p 44\u201347, vol 103\u2013110. Massey University, Palmerston North, New Zealand."},{"doi-asserted-by":"publisher","key":"e_1_3_3_33_2","DOI":"10.1146\/annurev.biochem.76.061005.092322"},{"doi-asserted-by":"publisher","key":"e_1_3_3_34_2","DOI":"10.7554\/eLife.42305"},{"doi-asserted-by":"publisher","key":"e_1_3_3_35_2","DOI":"10.1128\/JB.187.6.2218-2223.2005"},{"doi-asserted-by":"publisher","key":"e_1_3_3_36_2","DOI":"10.1016\/s1046-2023(03)00134-8"},{"doi-asserted-by":"publisher","key":"e_1_3_3_37_2","DOI":"10.1021\/acs.biochem.7b00268"},{"key":"e_1_3_3_38_2","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1002\/9781118876015.ch14","volume-title":"Natural products analysis: instrumentation, methods and applications","author":"Norris GE","year":"2014","unstructured":"Norris GE, Patchett ML. 2014. Glycosylated ribosomally synthesized peptide toxins: discovery, characterization and applications, p 507\u2013543. In Havl\u03af\u010dek V, Sp\u03af\u017eek J (ed), Natural products analysis: instrumentation, methods and applications. John Wiley & Sons, Inc., Hoboken, NJ."},{"doi-asserted-by":"publisher","key":"e_1_3_3_39_2","DOI":"10.1073\/pnas.1600494113"},{"doi-asserted-by":"publisher","key":"e_1_3_3_40_2","DOI":"10.3390\/ijms19010136"},{"doi-asserted-by":"publisher","key":"e_1_3_3_41_2","DOI":"10.1074\/jbc.M110.194506"},{"doi-asserted-by":"publisher","key":"e_1_3_3_42_2","DOI":"10.1016\/j.cell.2009.02.041"},{"doi-asserted-by":"publisher","key":"e_1_3_3_43_2","DOI":"10.1111\/j.1365-2958.2007.05707.x"},{"doi-asserted-by":"publisher","key":"e_1_3_3_44_2","DOI":"10.1016\/0014-5793(96)00459-0"},{"doi-asserted-by":"publisher","key":"e_1_3_3_45_2","DOI":"10.1016\/j.ab.2018.11.005"},{"doi-asserted-by":"publisher","key":"e_1_3_3_46_2","DOI":"10.1016\/j.mimet.2008.02.013"},{"doi-asserted-by":"publisher","key":"e_1_3_3_47_2","DOI":"10.1002\/pro.2253"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.00529-20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.00529-20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T20:27:02Z","timestamp":1697574422000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.00529-20"}},"subtitle":[],"editor":[{"given":"William W.","family":"Metcalf","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2021,3,8]]},"references-count":46,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2021,3,8]]}},"alternative-id":["10.1128\/JB.00529-20"],"URL":"https:\/\/doi.org\/10.1128\/jb.00529-20","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"type":"print","value":"0021-9193"},{"type":"electronic","value":"1098-5530"}],"subject":[],"published":{"date-parts":[[2021,3,8]]},"assertion":[{"value":"2020-09-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-03-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}