{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T01:46:28Z","timestamp":1791942388336,"version":"4.3.4"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"21","license":[{"start":{"date-parts":[[2010,11,1]],"date-time":"2010-11-01T00:00:00Z","timestamp":1288569600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The pathogenesis of<jats:italic>Brucella<\/jats:italic>is related to the ability to multiply intracellularly, an event controlled by the two-component system BvrR\/BvrS (TCS BvrRS) and the type IV secretion machinery VirB (T4SS VirB). We have hypothesized that the TCS BvrRS transcriptionally regulates the T4SS VirB. To test this hypothesis, we have compared the levels of VirB proteins in the wild-type strain<jats:italic>Brucella abortus<\/jats:italic>2308 and mutant strains devoid of the sensor and regulator genes (<jats:italic>bvrS<\/jats:italic>and<jats:italic>bvrR<\/jats:italic>mutants, respectively). While the<jats:italic>bvrR<\/jats:italic>and<jats:italic>bvrS<\/jats:italic>mutants showed low levels of the VirB1, VirB5, VirB8, and VirB9 proteins, the same proteins were overexpressed in the<jats:italic>bvrR<\/jats:italic>mutant complemented with a plasmid carrying a functional<jats:italic>bvrR<\/jats:italic>gene. Quantitation of<jats:italic>virB5<\/jats:italic>mRNA confirmed these data and indicated that the influence of the TCS BvrRS on the T4SS VirB occurs at the transcriptional level. The expression of the transcriptional activator VjbR also depended on the TCS BvrRS. In addition, we demonstrate a direct interaction between the promoter region of the VirB operon and the response regulator BvrR. Altogether these data demonstrate that the TCS BvrRS controls the expression of the T4SS VirB through direct and indirect mechanisms.<\/jats:p>","DOI":"10.1128\/jb.00567-10","type":"journal-article","created":{"date-parts":[[2010,9,11]],"date-time":"2010-09-11T03:09:05Z","timestamp":1284174545000},"page":"5603-5608","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":67,"title":["The Two-Component System BvrR\/BvrS Regulates the Expression of the Type IV Secretion System VirB in<i>Brucella abortus<\/i>"],"prefix":"10.1128","volume":"192","author":[{"given":"Carola","family":"Marti\u0301nez-Nu\u0301n\u0303ez","sequence":"first","affiliation":[{"name":"Programa de Investigacio\u0301n en Enfermedades Tropicales (PIET), Escuela de Medicina Veterinaria, Universidad Nacional, 304-3000 Heredia, Costa Rica"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela","family":"Altamirano-Silva","sequence":"additional","affiliation":[{"name":"Programa de Investigacio\u0301n en Enfermedades Tropicales (PIET), Escuela de Medicina Veterinaria, Universidad Nacional, 304-3000 Heredia, Costa Rica"},{"name":"Escuela de Biologi\u0301a, Universidad Nacional, 304-3000 Heredia, Costa Rica"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Alvarado-Guille\u0301n","sequence":"additional","affiliation":[{"name":"Programa de Investigacio\u0301n en Enfermedades Tropicales (PIET), Escuela de Medicina Veterinaria, Universidad Nacional, 304-3000 Heredia, Costa Rica"},{"name":"Facultad de Farmacia, Universidad de Costa Rica, 1000 San Jose\u0301, Costa Rica"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edgardo","family":"Moreno","sequence":"additional","affiliation":[{"name":"Programa de Investigacio\u0301n en Enfermedades Tropicales (PIET), Escuela de Medicina Veterinaria, Universidad Nacional, 304-3000 Heredia, Costa Rica"},{"name":"Instituto Clodomiro Picado, Facultad de Microbiologi\u0301a, Universidad de Costa Rica, 1000 San Jose\u0301, Costa Rica"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caterina","family":"Guzma\u0301n-Verri","sequence":"additional","affiliation":[{"name":"Programa de Investigacio\u0301n en Enfermedades Tropicales (PIET), Escuela de Medicina Veterinaria, Universidad Nacional, 304-3000 Heredia, Costa Rica"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esteban","family":"Chaves-Olarte","sequence":"additional","affiliation":[{"name":"Programa de Investigacio\u0301n en Enfermedades Tropicales (PIET), Escuela de Medicina Veterinaria, Universidad Nacional, 304-3000 Heredia, Costa Rica"},{"name":"Centro de Investigacio\u0301n en Enfermedades Tropicales, Facultad de Microbiologi\u0301a, Universidad de Costa Rica, 1000 San Jose\u0301, Costa Rica"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01551-07"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00232-10"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"e631","DOI":"10.1371\/journal.pone.0000631","volume":"2","year":"2007","unstructured":"Barquero-Calvo, E., E. 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