{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T23:40:26Z","timestamp":1791416426584,"version":"4.3.3"},"reference-count":65,"publisher":"American Society for Microbiology","issue":"18","license":[{"start":{"date-parts":[[2007,9,15]],"date-time":"2007-09-15T00:00:00Z","timestamp":1189814400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2007,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We applied a novel negative selection strategy called genomic array footprinting (GAF) to identify genes required for genetic transformation of the gram-positive bacterium\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            . Genome-wide\n            <jats:italic>mariner<\/jats:italic>\n            transposon mutant libraries in\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            strain R6 were challenged by transformation with an antibiotic resistance cassette and growth in the presence of the corresponding antibiotic. The GAF screen identified the enrichment of mutants in two genes, i.e.,\n            <jats:italic>hexA<\/jats:italic>\n            and\n            <jats:italic>hexB<\/jats:italic>\n            , and the counterselection of mutants in 21 different genes during the challenge. Eight of the counterselected genes were known to be essential for pneumococcal transformation. Four other genes, i.e.,\n            <jats:italic>radA<\/jats:italic>\n            ,\n            <jats:italic>comGF<\/jats:italic>\n            ,\n            <jats:italic>parB<\/jats:italic>\n            , and spr2011, have previously been linked to the competence regulon, and one, spr2014, was located adjacent to the essential competence gene\n            <jats:italic>comFA<\/jats:italic>\n            . Directed mutants of seven of the eight remaining genes, i.e., spr0459-spr0460, spr0777, spr0838, spr1259-spr1260, and spr1357, resulted in reduced, albeit modest, transformation rates. No connection to pneumococcal transformation could be made for the eighth gene, which encodes the response regulator RR03. We further demonstrated that the gene encoding the putative DNA repair protein RadA is required for efficient transformation with chromosomal markers, whereas transformation with replicating plasmid DNA was not significantly affected. The\n            <jats:italic>radA<\/jats:italic>\n            mutant also displayed an increased sensitivity to treatment with the DNA-damaging agent methyl methanesulfonate. Hence, RadA is considered to have a role in recombination of donor DNA and in DNA damage repair in\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jb.00573-07","type":"journal-article","created":{"date-parts":[[2007,7,14]],"date-time":"2007-07-14T00:59:53Z","timestamp":1184374793000},"page":"6540-6550","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":81,"title":["Search for Genes Essential for Pneumococcal Transformation: the RadA DNA Repair Protein Plays a Role in Genomic Recombination of Donor DNA"],"prefix":"10.1128","volume":"189","author":[{"given":"Peter","family":"Burghout","sequence":"first","affiliation":[{"name":"Laboratory of Pediatric Infectious Diseases, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hester J.","family":"Bootsma","sequence":"additional","affiliation":[{"name":"Laboratory of Pediatric Infectious Diseases, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomas G.","family":"Kloosterman","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, University of Groningen, Haren, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jetta J. E.","family":"Bijlsma","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, University of Groningen, Haren, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christa E.","family":"de Jongh","sequence":"additional","affiliation":[{"name":"Laboratory of Pediatric Infectious Diseases, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar P.","family":"Kuipers","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, University of Groningen, Haren, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter W. M.","family":"Hermans","sequence":"additional","affiliation":[{"name":"Laboratory of Pediatric Infectious Diseases, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1006\/jmbi.1994.1472","volume":"241","year":"1994","unstructured":"Alloing, G., P. de Philip, and J. P. Claverys. 1994. Three highly homologous membrane-bound lipoproteins participate in oligopeptide transport by the Ami system of the gram-positive Streptococcus pneumoniae. J. Mol. Biol. 241 : 44-58.","journal-title":"J. Mol. Biol."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1996.tb02556.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1016\/j.jmb.2006.04.007","volume":"359","year":"2006","unstructured":"Andersen, N. M., and S. Douthwaite. 2006. YebU is a m5C methyltransferase specific for 16S rRNA nucleotide 1407. J. Mol. Biol. 359 : 777-786.","journal-title":"J. Mol. Biol."},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1038\/nbt1101-1060","volume":"19","year":"2001","unstructured":"Badarinarayana, V., P. W. Estep III, J. Shendure, J. Edwards, S. Tavazoie, F. Lam, and G. M. Church. 2001. Selection analyses of insertional mutants using subgenic-resolution arrays. Nat. Biotechnol. 19 : 1060-1065.","journal-title":"Nat. Biotechnol."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.162.3.979-984.1985"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1046\/j.1365-2958.2001.02218.x","volume":"39","year":"2001","unstructured":"Bartilson, M., A. Marra, J. Christine, J. S. Asundi, W. P. Schneider, and A. E. Hromockyj. 2001. Differential fluorescence induction reveals Streptococcus pneumoniae loci regulated by competence stimulatory peptide. Mol. Microbiol. 39 : 126-135.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1111\/j.1574-695X.2007.00246.x","volume":"50","year":"2007","unstructured":"Battig, P., and K. Muhlemann. 2007. Capsule genes of Streptococcus pneumoniae influence growth in vitro. FEMS Immunol. Med. Microbiol. 50 : 324-329.","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.24.6836-6844.2002"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.21.6211-6222.1995"},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"e31","DOI":"10.1371\/journal.pgen.0020031","volume":"2","year":"2006","unstructured":"Bentley, S. D., D. M. Aanensen, A. Mavroidi, D. Saunders, E. Rabbinowitsch, M. Collins, K. Donohoe, D. Harris, L. Murphy, M. A. Quail, G. Samuel, I. C. Skovsted, M. S. Kaltoft, B. Barrell, P. R. Reeves, J. Parkhill, and B. G. Spratt. 2006. Genetic analysis of the capsular biosynthetic locus from all 90 pneumococcal serotypes. PLoS Genet. 2 : e31.","journal-title":"PLoS Genet."},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1046\/j.1365-2958.2002.03013.x","volume":"45","year":"2002","unstructured":"Berge, M., M. Moscoso, M. Prudhomme, B. Martin, and J. P. Claverys. 2002. Uptake of transforming DNA in gram-positive bacteria: a view from Streptococcus pneumoniae. Mol. Microbiol. 45 : 411-421.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01900-06"},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1046\/j.1365-2958.1998.00737.x","volume":"27","year":"1998","unstructured":"Campbell, E. A., S. Y. Choi, and H. R. Masure. 1998. A competence regulon in Streptococcus pneumoniae revealed by genomic analysis. Mol. Microbiol. 27 : 929-939.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1007\/s00438-001-0616-7","volume":"266","year":"2002","unstructured":"Carrasco, B., S. Fernandez, K. Asai, N. Ogasawara, and J. C. Alonso. 2002. Effect of the recU suppressors sms and subA on DNA repair and homologous recombination in Bacillus subtilis. Mol. Genet. Genomics 266 : 899-906.","journal-title":"Mol. Genet. Genomics"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.50.2.133-165.1986"},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/BF00334098","volume":"196","year":"1984","unstructured":"Claverys, J. P., H. Prats, H. Vasseghi, and M. Gherardi. 1984. Identification of Streptococcus pneumoniae mismatch repair genes by an additive transformation approach. Mol. Gen. Genet. 196 : 91-96.","journal-title":"Mol. Gen. Genet."},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1146\/annurev.micro.60.080805.142139","volume":"60","year":"2006","unstructured":"Claverys, J. P., M. Prudhomme, and B. Martin. 2006. Induction of competence regulons as a general response to stress in gram-positive bacteria. Annu. Rev. Microbiol. 60 : 451-475.","journal-title":"Annu. Rev. Microbiol."},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1111\/j.1365-2958.2003.03892.x","volume":"51","year":"2004","unstructured":"Dagkessamanskaia, A., M. Moscoso, V. Henard, S. Guiral, K. Overweg, M. Reuter, B. Martin, J. Wells, and J. P. Claverys. 2004. Interconnection of competence, stress and CiaR regulons in Streptococcus pneumoniae: competence triggers stationary phase autolysis of ciaR mutant cells. Mol. Microbiol. 51 : 1071-1086.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1046\/j.1365-2958.1997.5111879.x","volume":"25","year":"1997","unstructured":"Dintilhac, A., G. Alloing, C. Granadel, and J. P. Claverys. 1997. Competence and virulence of Streptococcus pneumoniae: Adc and PsaA mutants exhibit a requirement for Zn and Mn resulting from inactivation of putative ABC metal permeases. Mol. Microbiol. 25 : 727-739.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/S0923-2508(97)87643-7","volume":"148","year":"1997","unstructured":"Dintilhac, A., and J. P. Claverys. 1997. The adc locus, which affects competence for genetic transformation in Streptococcus pneumoniae, encodes an ABC transporter with a putative lipoprotein homologous to a family of streptococcal adhesins. Res. Microbiol. 148 : 119-131.","journal-title":"Res. Microbiol."},{"key":"e_1_3_2_22_2","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1089\/10766290152044995","volume":"7","year":"2001","unstructured":"Dopazo, J., A. Mendoza, J. Herrero, F. Caldara, Y. Humbert, L. Friedli, M. Guerrier, E. Grand-Schenk, C. Gandin, M. de Francesco, A. Polissi, G. Buell, G. Feger, E. Garcia, M. Peitsch, and J. F. Garcia-Bustos. 2001. Annotated draft genomic sequence from a Streptococcus pneumoniae type 19F clinical isolate. Microb. Drug Resist. 7 : 99-125.","journal-title":"Microb. Drug Resist."},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1099\/00221287-144-2-433","volume":"144","year":"1998","unstructured":"Gasc, A. M., P. Giammarinaro, S. Richter, and M. Sicard. 1998. Organization around the dnaA gene of Streptococcus pneumoniae. Microbiology 144 : 433-439.","journal-title":"Microbiology"},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"8710","DOI":"10.1073\/pnas.0500879102","volume":"102","year":"2005","unstructured":"Guiral, S., T. J. Mitchell, B. Martin, and J. P. Claverys. 2005. Competence-programmed predation of noncompetent cells in the human pathogen Streptococcus pneumoniae: genetic requirements. Proc. Natl. Acad. Sci. USA 102 : 8710-8715.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(83)80284-8"},{"key":"e_1_3_2_26_2","first-page":"1389","volume":"45","year":"2002","unstructured":"Hava, D. L., and A. Camilli. 2002. Large-scale identification of serotype 4 Streptococcus pneumoniae virulence factors. Mol. Microbiol. 45 : 1389-1406.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_27_2","doi-asserted-by":"crossref","first-page":"11140","DOI":"10.1073\/pnas.92.24.11140","volume":"92","year":"1995","unstructured":"Havarstein, L. S., G. Coomaraswamy, and D. A. Morrison. 1995. An unmodified heptadecapeptide pheromone induces competence for genetic transformation in Streptococcus pneumoniae. Proc. Natl. Acad. Sci. USA 92 : 11140-11144.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_28_2","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1046\/j.1365-2958.1996.521416.x","volume":"21","year":"1996","unstructured":"Havarstein, L. S., P. Gaustad, I. F. Nes, and D. A. Morrison. 1996. Identification of the streptococcal competence-pheromone receptor. Mol. Microbiol. 21 : 863-869.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.10.2759-2762.1995"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.10.5889-5900.2000"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.19.5709-5717.2001"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.1.372-381.1991"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.7.3902-3906.2004"},{"key":"e_1_3_2_34_2","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1099\/mic.0.28521-0","volume":"152","year":"2006","unstructured":"Kloosterman, T. G., J. J. Bijlsma, J. Kok, and O. P. Kuipers. 2006. To have neighbour's fare: extending the molecular toolbox for Streptococcus pneumoniae. Microbiology 152 : 351-359.","journal-title":"Microbiology"},{"key":"e_1_3_2_35_2","doi-asserted-by":"crossref","first-page":"25097","DOI":"10.1074\/jbc.M601661200","volume":"281","year":"2006","unstructured":"Kloosterman, T. G., W. T. Hendriksen, J. J. Bijlsma, H. J. Bootsma, S. A. van Hijum, J. Kok, P. W. Hermans, and O. P. Kuipers. 2006. Regulation of glutamine and glutamate metabolism by GlnR and GlnA in Streptococcus pneumoniae. J. Biol. Chem. 281 : 25097-25109.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.123.1.222-232.1975"},{"key":"e_1_3_2_37_2","doi-asserted-by":"crossref","first-page":"11428","DOI":"10.1073\/pnas.96.20.11428","volume":"96","year":"1999","unstructured":"Lampe, D. J., B. J. Akerley, E. J. Rubin, J. J. Mekalanos, and H. M. Robertson. 1999. Hyperactive transposase mutants of the Himar1 mariner transposon. Proc. Natl. Acad. Sci. USA 96 : 11428-11433.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb00930.x"},{"key":"e_1_3_2_39_2","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/S0378-1119(99)00266-8","volume":"237","year":"1999","unstructured":"Lange, R., C. Wagner, A. de Saizieu, N. Flint, J. Molnos, M. Stieger, P. Caspers, M. Kamber, W. Keck, and K. E. Amrein. 1999. Domain organization and molecular characterization of 13 two-component systems identified by genome sequencing of Streptococcus pneumoniae. Gene 237 : 223-234.","journal-title":"Gene"},{"key":"e_1_3_2_40_2","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1046\/j.1365-2958.2001.02335.x","volume":"40","year":"2001","unstructured":"Lau, G. W., S. Haataja, M. Lonetto, S. E. Kensit, A. Marra, A. P. Bryant, D. McDevitt, D. A. Morrison, and D. W. Holden. 2001. A functional genomic analysis of type 3 Streptococcus pneumoniae virulence. Mol. Microbiol. 40 : 555-571.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.5.1883-1890.1999"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.16.5004-5016.1999"},{"key":"e_1_3_2_43_2","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1007\/s002530051192","volume":"49","year":"1998","unstructured":"Leenhouts, K., A. Bolhuis, G. Venema, and J. Kok. 1998. Construction of a food-grade multiple-copy integration system for Lactococcus lactis. Appl. Microbiol. Biotechnol. 49 : 417-423.","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"e_1_3_2_44_2","doi-asserted-by":"crossref","first-page":"19937","DOI":"10.1074\/jbc.M010192200","volume":"276","year":"2001","unstructured":"Long, A. D., H. J. Mangalam, B. Y. Chan, L. Tolleri, G. W. Hatfield, and P. Baldi. 2001. Improved statistical inference from DNA microarray data using analysis of variance and a Bayesian statistical framework. Analysis of global gene expression in Escherichia coli K12. J. Biol. Chem. 276 : 19937-19944.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_45_2","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1111\/j.1365-2958.1995.tb02250.x","volume":"15","year":"1995","unstructured":"Martin, B., P. Garcia, M. P. Castanie, and J. P. Claverys. 1995. The recA gene of Streptococcus pneumoniae is part of a competence-induced operon and controls lysogenic induction. Mol. Microbiol. 15 : 367-379.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.1.60-70.2003"},{"key":"e_1_3_2_47_2","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1038\/282215a0","volume":"282","year":"1979","unstructured":"Morrison, D. A., and M. F. Baker. 1979. Competence for genetic transformation in pneumococcus depends on synthesis of a small set of proteins. Nature 282 : 215-217.","journal-title":"Nature"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.159.3.870-876.1984"},{"key":"e_1_3_2_49_2","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1111\/j.1365-2958.2006.05310.x","volume":"61","year":"2006","unstructured":"Oggioni, M. R., C. Trappetti, A. Kadioglu, M. Cassone, F. Iannelli, S. Ricci, P. W. Andrew, and G. Pozzi. 2006. Switch from planktonic to sessile life: a major event in pneumococcal pathogenesis. Mol. Microbiol. 61 : 1196-1210.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_50_2","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/S0923-2508(02)01312-8","volume":"153","year":"2002","unstructured":"Pearce, B. J., F. Iannelli, and G. Pozzi. 2002. Construction of new unencapsulated (rough) strains of Streptococcus pneumoniae. Res. Microbiol. 153 : 243-247.","journal-title":"Res. Microbiol."},{"key":"e_1_3_2_51_2","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1111\/j.1365-2958.1994.tb01076.x","volume":"12","year":"1994","unstructured":"Pearce, B. J., A. M. Naughton, and H. R. Masure. 1994. Peptide permeases modulate transformation in Streptococcus pneumoniae. Mol. Microbiol. 12 : 881-892.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_52_2","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1046\/j.1365-2958.1996.501417.x","volume":"21","year":"1996","unstructured":"Pestova, E. V., L. S. Havarstein, and D. A. Morrison. 1996. Regulation of competence for genetic transformation in Streptococcus pneumoniae by an auto-induced peptide pheromone and a two-component regulatory system. Mol. Microbiol. 21 : 853-862.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.10.2701-2710.1998"},{"key":"e_1_3_2_54_2","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1046\/j.1365-2958.2003.03907.x","volume":"51","year":"2004","unstructured":"Peterson, S. N., C. K. Sung, R. Cline, B. V. Desai, E. C. Snesrud, P. Luo, J. Walling, H. Li, M. Mintz, G. Tsegaye, P. C. Burr, Y. Do, S. Ahn, J. Gilbert, R. D. Fleischmann, and D. A. Morrison. 2004. Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Mol. Microbiol. 51 : 1051-1070.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.12.5620-5629.1998"},{"key":"e_1_3_2_56_2","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1007\/BF00425735","volume":"200","year":"1985","unstructured":"Prats, H., B. Martin, and J. P. Claverys. 1985. The hexB mismatch repair gene of Streptococcus pneumoniae: characterisation, cloning and identification of the product. Mol. Gen. Genet. 200 : 482-489.","journal-title":"Mol. Gen. Genet."},{"key":"e_1_3_2_57_2","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1126\/science.1127912","volume":"313","year":"2006","unstructured":"Prudhomme, M., L. Attaiech, G. Sanchez, B. Martin, and J. P. Claverys. 2006. Antibiotic stress induces genetic transformability in the human pathogen Streptococcus pneumoniae. Science 313 : 89-92.","journal-title":"Science"},{"key":"e_1_3_2_58_2","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1016\/S0022-2836(05)80259-1","volume":"213","year":"1990","unstructured":"Puyet, A., B. Greenberg, and S. A. Lacks. 1990. Genetic and structural characterization of endA. A membrane-bound nuclease required for transformation of Streptococcus pneumoniae. J. Mol. Biol. 213 : 727-738.","journal-title":"J. Mol. Biol."},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.77.3.296-309.1959"},{"key":"e_1_3_2_60_2","doi-asserted-by":"crossref","first-page":"12712","DOI":"10.1073\/pnas.231275498","volume":"98","year":"2001","unstructured":"Sassetti, C. M., D. H. Boyd, and E. J. Rubin. 2001. Comprehensive identification of conditionally essential genes in mycobacteria. Proc. Natl. Acad. Sci. USA 98 : 12712-12717.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.12.3969-3979.2005"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.11.5190-5196.2001"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1061217"},{"key":"e_1_3_2_64_2","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1186\/1471-2164-6-77","volume":"6","year":"2005","unstructured":"van Hijum, S. A., A. de Jong, R. J. Baerends, H. A. Karsens, N. E. Kramer, R. Larsen, C. D. den Hengst, C. J. Albers, J. Kok, and O. P. Kuipers. 2005. A generally applicable validation scheme for the assessment of factors involved in reproducibility and quality of DNA-microarray data. BMC Genomics 6 : 77.","journal-title":"BMC Genomics"},{"key":"e_1_3_2_65_2","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/0076-6879(94)35141-4","volume":"235","year":"1994","unstructured":"van Soolingen, D., P. E. de Haas, P. W. Hermans, and J. D. van Embden. 1994. DNA fingerprinting of Mycobacterium tuberculosis. Methods Enzymol. 235 : 196-205.","journal-title":"Methods Enzymol."},{"key":"e_1_3_2_66_2","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1126\/science.8160014","volume":"264","year":"1994","unstructured":"Wu, L. J., and J. Errington. 1994. Bacillus subtilis SpoIIIE protein required for DNA segregation during asymmetric cell division. Science 264 : 572-575.","journal-title":"Science"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.00573-07","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.00573-07","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:35:14Z","timestamp":1627580114000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.00573-07"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,9,15]]},"references-count":65,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2007,9,15]]}},"alternative-id":["10.1128\/JB.00573-07"],"URL":"https:\/\/doi.org\/10.1128\/jb.00573-07","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,9,15]]},"assertion":[{"value":"2007-04-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-07-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}