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By genomic library tagging, we cloned a<jats:italic>dps<\/jats:italic>(stands for<jats:italic>D<\/jats:italic>NA-binding<jats:italic>p<\/jats:italic>rotein from<jats:italic>s<\/jats:italic>tarved cells) gene of the symbiotic \u201c<jats:italic>Candidatus<\/jats:italic>Legionella jeonii\u201d organism (called the X bacterium) (<jats:italic>dps<\/jats:italic><jats:sub>X<\/jats:sub>) that grows in<jats:italic>Amoeba proteus<\/jats:italic>. The gene encodes a 17-kDa protein (pI 5.19) with 91% homology to Dps and DNA-binding ferritin-like proteins of other organisms. The cloned gene complemented the<jats:italic>dps<\/jats:italic>mutant of<jats:italic>Escherichia coli<\/jats:italic>and conferred resistance to hydrogen peroxide. Dps<jats:sub>X<\/jats:sub>proteins purified from<jats:italic>E. coli<\/jats:italic>transformed with the<jats:italic>dps<\/jats:italic><jats:sub>X<\/jats:sub>gene were in oligomeric form, formed a complex with pBlueskript SKII DNA, and protected the DNA from DNase I digestion and H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>-mediated damage. The expression of the<jats:italic>dps<\/jats:italic><jats:sub>X<\/jats:sub>gene in \u201c<jats:italic>Candidatus<\/jats:italic>Legionella jeonii\u201d was enhanced when the host amoeba was treated with 2 mM H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>and by phagocytic activities of the host cell. These results suggested that the Dps protein has a function protective of the bacterial DNA and that its gene expression responds to oxidative stress generated by phagocytic activities of the host cell. With regard to the fact that invasion of<jats:italic>Legionella<\/jats:italic>sp. into respiratory phagocytic cells causes pneumonia in mammals, further characterization of<jats:italic>dps<\/jats:italic><jats:sub>X<\/jats:sub>expression in the<jats:italic>Legionella<\/jats:italic>sp. that multiplies in a protozoan host in the natural environment may provide valuable information toward understanding the protective mechanisms of intracellular pathogens.<\/jats:p>","DOI":"10.1128\/jb.00576-06","type":"journal-article","created":{"date-parts":[[2006,10,18]],"date-time":"2006-10-18T23:23:35Z","timestamp":1161213815000},"page":"7572-7580","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["The<i>dps<\/i>Gene of Symbiotic \u201c<i>Candidatus<\/i>Legionella jeonii\u201d in<i>Amoeba proteus<\/i>Responds to Hydrogen Peroxide and Phagocytosis"],"prefix":"10.1128","volume":"188","author":[{"given":"Miey","family":"Park","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Seoul National University, Seoul 151-742, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seong Tae","family":"Yun","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Seoul National University, Seoul 151-742, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sue-Yun","family":"Hwang","sequence":"additional","affiliation":[{"name":"Graduate School of Biology and Information Technology, Hankyong National University, 67 Sokjong-Dong, Kyonggi-Do 456-749, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Choong-Ill","family":"Chun","sequence":"additional","affiliation":[{"name":"Department of Biological Science, Sookmyung Women's University, Seoul 140-742, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tae In","family":"Ahn","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Seoul National University, Seoul 151-742, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"43","volume":"128","year":"1994","unstructured":"Ahn, T. 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