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Here, we show that\n                      <jats:italic toggle=\"yes\">gcvT<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">gcvP,<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">gcvH,<\/jats:italic>\n                      constituting specific components of the glycine cleavage system which converts glycine to carbon dioxide, ammonia, and 5,10-methylene tetrahydrofolate, are negatively regulated by the sibling sRNAs, while the fourth component of the GCV multienzyme complex, the L-protein, which is also involved in other cellular reactions, is not targeted by NgncR_162 and NgncR_163. In contrast to\n                      <jats:italic toggle=\"yes\">gcvP<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">gcvL<\/jats:italic>\n                      , the expression of\n                      <jats:italic toggle=\"yes\">gcvT<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">gcvH,<\/jats:italic>\n                      being part of a tricistronic operon, is upregulated in the presence of glycine via a translational tandem glycine riboswitch. In the absence of glycine, aptamer 2 contributes to the formation of an inhibitory secondary structure that sequesters the ribosomal-binding site of\n                      <jats:italic toggle=\"yes\">gcvT,<\/jats:italic>\n                      which notably is also targeted by the sibling sRNAs. To our knowledge, this is the first example of dual control by a riboswitch and an sRNA where the same segment of the target mRNA is involved in base-pairing interactions with both the cis- and the trans-acting riboregulator, suggesting considerable structural flexibility of the\n                      <jats:italic toggle=\"yes\">gcvT<\/jats:italic>\n                      -NGFG_01513-\n                      <jats:italic toggle=\"yes\">gcvH<\/jats:italic>\n                      mRNA.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      In the bacterial world, riboregulation is extremely widespread and controls virtually all aspects of the life of bacterial cells. Riboregulatory mechanisms are very diverse and involve\n                      <jats:italic toggle=\"yes\">trans<\/jats:italic>\n                      - and\n                      <jats:italic toggle=\"yes\">cis<\/jats:italic>\n                      -acting regulatory elements such as small RNAs and riboswitches that are able to control gene expression by direct binding of small molecules. Here, we report a very peculiar and rare example of riboregulation in which an mRNA is regulated by both\n                      <jats:italic toggle=\"yes\">trans<\/jats:italic>\n                      -acting sibling sRNAs and a glycine-responsive tandem riboswitch, which control glycine metabolism of the human pathogen\n                      <jats:italic toggle=\"yes\">Neisseria gonorrhoeae<\/jats:italic>\n                      . This regulatory interplay of sibling sRNAs with a tandem riboswitch allows integration of different cellular signals and represents an intriguing new facet of riboregulation.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/jb.00593-25","type":"journal-article","created":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T15:54:26Z","timestamp":1772726066000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Complex interplay of riboregulatory mechanisms at the\n                    <i>gcvT<\/i>\n                    -NGFG_01513-\n                    <i>gcvH<\/i>\n                    operon of\n                    <i>Neisseria gonorrhoeae<\/i>"],"prefix":"10.1128","volume":"208","author":[{"given":"Susanne","family":"Bauer","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00fbnyb24","id-type":"ROR","asserted-by":"publisher"}],"name":"Chair of Microbiology, 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