{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T12:23:20Z","timestamp":1785932600521,"version":"3.56.0"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2009,3,1]],"date-time":"2009-03-01T00:00:00Z","timestamp":1235865600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2009,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The gram-negative, strictly anaerobic epsilonproteobacterium\n            <jats:italic>Sulfurospirillum multivorans<\/jats:italic>\n            is able to gain energy from dehalorespiration with tetrachloroethene (perchloroethylene [PCE]) as a terminal electron acceptor. The organism can also utilize fumarate as an electron acceptor. Prolonged subcultivation of\n            <jats:italic>S. multivorans<\/jats:italic>\n            in the absence of PCE with pyruvate as an electron donor and fumarate as an electron acceptor resulted in a decrease of PCE dehalogenase (PceA) activity. Concomitantly, the\n            <jats:italic>pceA<\/jats:italic>\n            transcript level equally decreased as shown by reverse transcriptase PCR. After 35 subcultivations (approximately 105 generations), a\n            <jats:italic>pceA<\/jats:italic>\n            transcript was not detectable and the PceA protein and activity were completely absent. In such long-term subcultivated\n            <jats:italic>S. multivorans<\/jats:italic>\n            cells, the biosynthesis of catalytically active PceA was restored to the initial level within about 50 h (approximately three generations) by the addition of PCE or trichloroethene. Single colonies obtained from PceA-depleted cultures were able to induce PCE dechlorination, indicating that long-term subcultured cells still contained the functional\n            <jats:italic>pceA<\/jats:italic>\n            gene. The results point to a novel type of long-term regulation of PCE dehalogenase gene expression in\n            <jats:italic>S. multivorans<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jb.00597-08","type":"journal-article","created":{"date-parts":[[2008,12,23]],"date-time":"2008-12-23T01:58:20Z","timestamp":1229997500000},"page":"1650-1655","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["Retentive Memory of Bacteria: Long-Term Regulation of Dehalorespiration in\n            <i>Sulfurospirillum multivorans<\/i>"],"prefix":"10.1128","volume":"191","author":[{"given":"Markus","family":"John","sequence":"first","affiliation":[{"name":"Friedrich-Schiller University, Institute for Microbiology, Philosophenweg 12, D-07743 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raffael","family":"Rubick","sequence":"additional","affiliation":[{"name":"Friedrich-Schiller University, Institute for Microbiology, Philosophenweg 12, D-07743 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland P. H.","family":"Schmitz","sequence":"additional","affiliation":[{"name":"Friedrich-Schiller University, Institute for Microbiology, Philosophenweg 12, D-07743 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Rakoczy","sequence":"additional","affiliation":[{"name":"Friedrich-Schiller University, Institute for Microbiology, Philosophenweg 12, D-07743 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Torsten","family":"Schubert","sequence":"additional","affiliation":[{"name":"Friedrich-Schiller University, Institute for Microbiology, Philosophenweg 12, D-07743 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"Diekert","sequence":"additional","affiliation":[{"name":"Friedrich-Schiller University, Institute for Microbiology, Philosophenweg 12, D-07743 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Current protocols in molecular biology. 1987"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1046\/j.1365-2958.1996.00114.x","volume":"22","year":"1996","unstructured":"Berks, B. 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