{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T21:28:15Z","timestamp":1790112495923,"version":"4.0.1"},"reference-count":59,"publisher":"American Society for Microbiology","issue":"22","license":[{"start":{"date-parts":[[2015,11,15]],"date-time":"2015-11-15T00:00:00Z","timestamp":1447545600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2015,11,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Hydrogen sulfide (H\n                    <jats:sub>2<\/jats:sub>\n                    S), well known for its toxic properties, has recently become a research focus in bacteria, in part because it has been found to prevent oxidative stress caused by treatment with some antibiotics. H\n                    <jats:sub>2<\/jats:sub>\n                    S has the ability to scavenge reactive oxygen species (ROS), thus preventing oxidative stress, but it is also toxic, leading to conflicting reports of its effects in different organisms. Here, with\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Shewanella oneidensis<\/jats:named-content>\n                    as a model, we report that the effects of H\n                    <jats:sub>2<\/jats:sub>\n                    S on the response to oxidative stress are time dependent. When added simultaneously with H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    , H\n                    <jats:sub>2<\/jats:sub>\n                    S promoted H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    toxicity by inactivating catalase, KatB, a heme-containing enzyme involved in H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    degradation. Such an inhibitory effect may apply to other heme-containing proteins, such as cytochrome\n                    <jats:italic>cbb<\/jats:italic>\n                    <jats:sub>3<\/jats:sub>\n                    oxidase. When H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    was supplied 20 min or later after the addition of H\n                    <jats:sub>2<\/jats:sub>\n                    S, the oxidative-stress-responding regulator OxyR was activated, resulting in increased resistance to H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    . The activation of OxyR was likely triggered by the influx of iron, a response to lowered intracellular iron due to the iron-sequestering property of H\n                    <jats:sub>2<\/jats:sub>\n                    S. Given that\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Shewanella<\/jats:named-content>\n                    bacteria thrive in redox-stratified environments that have abundant sulfur and iron species, our results imply that H\n                    <jats:sub>2<\/jats:sub>\n                    S is more important for bacterial survival in such environmental niches than previously believed.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>IMPORTANCE<\/jats:bold>\n                    Previous studies have demonstrated that H\n                    <jats:sub>2<\/jats:sub>\n                    S is either detrimental or beneficial to bacterial cells. While it can act as a growth-inhibiting molecule by damaging DNA and denaturing proteins, it helps cells to combat oxidative stress. Here we report that H\n                    <jats:sub>2<\/jats:sub>\n                    S indeed has these contrasting biological functions and that its effects are time dependent. Immediately after H\n                    <jats:sub>2<\/jats:sub>\n                    S treatment, there is growth inhibition due to damage of heme-containing proteins, at least to catalase and cytochrome\n                    <jats:italic>c<\/jats:italic>\n                    oxidase. In contrast, when added a certain time later, H\n                    <jats:sub>2<\/jats:sub>\n                    S confers an enhanced ability to combat oxidative stress by activating the H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    -responding regulator OxyR. Our data reconcile conflicting observations about the functions of H\n                    <jats:sub>2<\/jats:sub>\n                    S.\n                  <\/jats:p>","DOI":"10.1128\/jb.00603-15","type":"journal-article","created":{"date-parts":[[2015,8,31]],"date-time":"2015-08-31T21:59:56Z","timestamp":1441058396000},"page":"3563-3572","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["A Matter of Timing: Contrasting Effects of Hydrogen Sulfide on Oxidative Stress Response in Shewanella oneidensis"],"prefix":"10.1128","volume":"197","author":[{"given":"Genfu","family":"Wu","sequence":"first","affiliation":[{"name":"Institute of Microbiology and College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fen","family":"Wan","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huihui","family":"Fu","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haichun","family":"Gao","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00017.2011"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2013.5309"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1209855"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2004.05.020"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00047-05"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10565-011-9198-2"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-014-0968-0"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0104206"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1947"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-092611-150104"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.3109\/1040841X.2012.726209"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.240.4857.1319"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00888-09"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2010.02313.x"},{"key":"e_1_3_3_16_2","first-page":"374","article-title":"Endogenous generation of hydrogen sulfide and its regulation in Shewanella oneidensis","volume":"6","author":"Wu G","year":"2015","unstructured":"Wu G, Li N, Mao Y, Zhou G, Gao H. 2015. 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