{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T19:01:23Z","timestamp":1791313283037,"version":"4.3.1"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"21","license":[{"start":{"date-parts":[[2010,11,1]],"date-time":"2010-11-01T00:00:00Z","timestamp":1288569600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The SasG surface protein of\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            has been shown to promote the formation of biofilm. SasG comprises an N-terminal A domain and repeated B domains. Here we demonstrate that SasG is involved in the accumulation phase of biofilm, a process that requires a physiological concentration of Zn\n            <jats:sup>2+<\/jats:sup>\n            . The B domains, but not the A domain, are required. Purified recombinant B domain protein can form dimers\n            <jats:italic>in vitro<\/jats:italic>\n            in a Zn\n            <jats:sup>2+<\/jats:sup>\n            -dependent fashion. Furthermore, the protein can bind to cells that have B domains anchored to their surface and block biofilm formation. The full-length SasG protein exposed on the cell surface is processed within the B domains to a limited degree, resulting in cleaved proteins of various lengths being released into the supernatant. Some of the released molecules associate with the surface-exposed B domains that remain attached to the cell. Studies using inhibitors and mutants failed to identify any protease that could cause the observed cleavage within the B domains. Extensively purified recombinant B domain protein is very labile, and we propose that cleavage occurs spontaneously at labile peptide bonds and that this is necessary for biofilm formation.\n          <\/jats:p>","DOI":"10.1128\/jb.00628-10","type":"journal-article","created":{"date-parts":[[2010,9,4]],"date-time":"2010-09-04T02:13:26Z","timestamp":1283566406000},"page":"5663-5673","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":208,"title":["Role of Surface Protein SasG in Biofilm Formation by\n            <i>Staphylococcus aureus<\/i>"],"prefix":"10.1128","volume":"192","author":[{"given":"Joan A.","family":"Geoghegan","sequence":"first","affiliation":[{"name":"Microbiology Department, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca M.","family":"Corrigan","sequence":"additional","affiliation":[{"name":"Microbiology Department, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominika T.","family":"Gruszka","sequence":"additional","affiliation":[{"name":"Department of Biology, University of York, York YO10 5DD, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pietro","family":"Speziale","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Viale Taramelli 3\/b, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James P.","family":"O'Gara","sequence":"additional","affiliation":[{"name":"UCD School of Biomolecular and Biomedical Sciences, University College Dublin, Dublin 4, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer R.","family":"Potts","sequence":"additional","affiliation":[{"name":"Department of Biology, University of York, York YO10 5DD, United Kingdom"},{"name":"Department of Chemistry, University of York, Heslington, York YO10 5DD, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy J.","family":"Foster","sequence":"additional","affiliation":[{"name":"Microbiology Department, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/j.1432-1033.1986.tb09933.x","volume":"160","year":"1986","unstructured":"Allgaier, H., G. 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