{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T03:25:22Z","timestamp":1791343522998,"version":"4.3.1"},"reference-count":15,"publisher":"American Society for Microbiology","issue":"21","license":[{"start":{"date-parts":[[2006,11,1]],"date-time":"2006-11-01T00:00:00Z","timestamp":1162339200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>B. melitensis<\/jats:italic>16M genome analysis revealed the presence of six putative sigma factor-encoding genes:<jats:italic>rpoD<\/jats:italic>,<jats:italic>rpoH1<\/jats:italic>,<jats:italic>rpoH2<\/jats:italic>,<jats:italic>rpoE1<\/jats:italic>,<jats:italic>rpoE2<\/jats:italic>, and<jats:italic>rpoN<\/jats:italic>. We mutated all these genes except<jats:italic>rpoD<\/jats:italic>. Phenotypic analysis of the mutants reveals that a strain carrying an<jats:italic>rpoH2<\/jats:italic>null mutation (\u0394<jats:italic>rpoH2<\/jats:italic>) is impaired for growth at 21 and 42\u00b0C and shows increased sensitivity to hydrogen peroxide. Compared to the wild-type strain, the \u0394<jats:italic>rpoH2<\/jats:italic>mutant is attenuated in all virulence models tested. Three other null mutants (\u0394<jats:italic>rpoH1<\/jats:italic>, \u0394<jats:italic>rpoE1<\/jats:italic>, and \u0394<jats:italic>rpoE2<\/jats:italic>mutants) are also defective for survival in mice at 4 weeks postinfection. We also demonstrated that<jats:italic>rpoH2<\/jats:italic>deletion strongly reduces the expression of two major virulence factors in<jats:italic>B. melitensis<\/jats:italic>, the type IV secretion system and the flagellum.<\/jats:p>","DOI":"10.1128\/jb.00644-06","type":"journal-article","created":{"date-parts":[[2006,10,18]],"date-time":"2006-10-18T23:23:35Z","timestamp":1161213815000},"page":"7707-7710","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":66,"title":["An RpoH-Like Heat Shock Sigma Factor Is Involved in Stress Response and Virulence in<i>Brucella melitensis<\/i>16M"],"prefix":"10.1128","volume":"188","author":[{"given":"Marie","family":"Delory","sequence":"first","affiliation":[{"name":"Research Unit in Molecular Biology, University of Namur, Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Re\u0301gis","family":"Hallez","sequence":"additional","affiliation":[{"name":"Research Unit in Molecular Biology, University of Namur, Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Jacques","family":"Letesson","sequence":"additional","affiliation":[{"name":"Research Unit in Molecular Biology, University of Namur, Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"De Bolle","sequence":"additional","affiliation":[{"name":"Research Unit in Molecular Biology, University of Namur, Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2005.00543.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.7.2320-2328.1990"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.10.4135-4139.1994"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"11978","DOI":"10.1073\/pnas.89.24.11978","volume":"89","year":"1992","unstructured":"Fang, F. 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