{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T10:30:16Z","timestamp":1716373816757},"reference-count":60,"publisher":"American Society for Microbiology","issue":"21","license":[{"start":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T00:00:00Z","timestamp":1383264000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The importance of gene regulation in the enzootic cycle of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Borrelia burgdorferi<\/jats:named-content>\n            , the spirochete that causes Lyme disease, is well established.\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. burgdorferi<\/jats:named-content>\n            regulates gene expression in response to changes in environmental stimuli associated with changing hosts. In this study, we monitored mRNA decay in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. burgdorferi<\/jats:named-content>\n            following transcriptional arrest with actinomycin D. The time-dependent decay of transcripts encoding RNA polymerase subunits (\n            <jats:italic>rpoA<\/jats:italic>\n            and\n            <jats:italic>rpoS<\/jats:italic>\n            ), ribosomal proteins (\n            <jats:italic>rpsD<\/jats:italic>\n            ,\n            <jats:italic>rpsK<\/jats:italic>\n            ,\n            <jats:italic>rpsM<\/jats:italic>\n            ,\n            <jats:italic>rplQ<\/jats:italic>\n            , and\n            <jats:italic>rpsO<\/jats:italic>\n            ), a nuclease (\n            <jats:italic>pnp<\/jats:italic>\n            ), outer surface lipoproteins (\n            <jats:italic>ospA<\/jats:italic>\n            and\n            <jats:italic>ospC<\/jats:italic>\n            ), and a flagellar protein (\n            <jats:italic>flaB<\/jats:italic>\n            ) have different profiles and indicate half-lives ranging from approximately 1 min to more than 45 min in cells cultured at 35\u00b0C. Our results provide a first step in characterizing mRNA decay in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. burgdorferi<\/jats:named-content>\n            and in investigating its role in gene expression and regulation.\n          <\/jats:p>","DOI":"10.1128\/jb.00659-13","type":"journal-article","created":{"date-parts":[[2013,8,24]],"date-time":"2013-08-24T01:47:55Z","timestamp":1377308875000},"page":"4879-4887","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Measurements of mRNA Degradation in Borrelia burgdorferi"],"prefix":"10.1128","volume":"195","author":[{"given":"Linda","family":"Archambault","sequence":"first","affiliation":[{"name":"Program in Biological Chemistry, Bates College, Lewiston, Maine, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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